A model to study total hepatic ischemia–reperfusion injury

Kanoria, Sanjeev; Glantzounis, George; Jalan, R; Davies, Nicola; Seifalian, Alexander M.; Williams, Ruth; Davidson, Brian R

doi:10.1016/j.transproceed.2004.10.031

Cited by 27 publications

(24 citation statements)

References 10 publications

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“…In this experiment, the MDA level in hepatic tissues after hepatic ischemia/reperfusion was shown to increase, which suggests that notable damage during hepatic ischemia/reperfusion is caused by free radicals. This observation is consistent with results from previous studies on ischemia/reperfusion [16,17]. During lipid peroxidation, some volatile saturated alkanes were also produced in addition to MDA.…”

Section: Discussionsupporting

confidence: 93%

Measurement of pentane in expiratory gas during rabbit hepatic ischemia/reperfusion by solid-phase microextraction and gas chromatography–mass spectrometry (SPME GC/MS)

Liu¹,

Shi²,

Wang³

et al. 2012

J. Breath Res.

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The aim of this study was to determine the changes in the pentane concentration of expiratory gas as well as the relationship between this pentane concentration and hepatic oxidative stress during rabbit hepatic ischemia/reperfusion using solid-phase microextraction (SPME) and gas chromatography-mass spectrometry (GC/MS). 45 white male rabbits with body weights between 2.5 and 3.0 kg were randomly assigned to the following three groups: the 10 min ischemia group (group S); the 20 min ischemia group (group M); or the 30 min ischemia group (group L). Expiratory gases were collected prior to ischemia (T0) and for 1, 10, 20, 30, 60 and 120 min (T1-T6) following reperfusion. Pentane concentrations were determined using SPME and GC/MS. In addition, arterial blood samples were collected, and serum aminotransferase (AST) and malondialdehyde (MDA) concentrations were measured. In the three groups, the pentane concentrations of the expiratory gases at points T1 and T2 were significantly increased (P < 0.05) compared with those at point T0, and the serum AST and MDA concentrations at points T5 and T6 were also significantly increased (P < 0.05) compared with those at point T0. Therefore, the use of SPME in combination with GC/MS represents an improved anesthesia system that can be used to continuously measure the concentration of pentane in expiratory gases, which can reflect the degree of oxidative stress during hepatic ischemia/reperfusion.

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Section: Discussionsupporting

confidence: 93%

Measurement of pentane in expiratory gas during rabbit hepatic ischemia/reperfusion by solid-phase microextraction and gas chromatography–mass spectrometry (SPME GC/MS)

Liu¹,

Shi²,

Wang³

et al. 2012

J. Breath Res.

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“…14 A previous study on ischemia-reperfusion injury in NZW rabbits found that an ischemia time of 20 min did not produce significant liver injury whereas an ischemia time of 30 min caused severe liver injury. 15 Although the primary objective to administer N-acetylcysteine in the current study was the prevention of reperfusion injury after warm hepatic ischemia, 16 N-acetylcysteine may have positive effects on the diameter of fenestrae by increasing the bioavailability of NO. 17 Large species variations of the diameter of sinusoidal fenestrae between cow, pig, guinea pig, sheep and dog were demonstrated by Higashi et al 18 The average diameter obtained by scanning electron microscopy was 45 nm in cows, 52 nm in sheep, 66 nm in guineapig, 82 nm in pig and 131 nm in dog but the confidence intervals for each species were large.…”

Section: Discussionmentioning

confidence: 94%

Species differences in transgene DNA uptake in hepatocytes after adenoviral transfer correlate with the size of endothelial fenestrae

et al. 2007

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“…Was observed that these animals tolerate ischemia for 60 minutes with low mortality and submitting them to intermittent ischemia produces no mortality 18 . Different models of intermittent ischemia have been described and was chosen one of them in this study, considering that after 10 to 12 minutes of ischemia, three to five minutes of reperfusion are sufficient to restore the intra-hepatic tissue oxygenation 6,23,28 .…”

Section: Discussionmentioning

confidence: 99%

“…Available papers in literature that study I/R injury in the liver with animal models are numerous and focus on different variables including the animal model, method and duration of ischemia, intermittent ischemia, ischemic preconditioning and remote ischemic preconditioning 18,19 . This study aimed to identify, between two methods of clamping the hepatic pedicle (methods that present technical differences, with advantages and disadvantages in both sides), which one produces less liver damage.…”

Section: Discussionmentioning

confidence: 99%

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Lesão de isquemia e reperfusão após clampagem contínua ou intermitente do pedículo hepático em coelhos

Seabra

Savassi-Rocha

Vasconcelos

et al. 2012

ABCD, arq. bras. cir. dig.

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-Background -The control of bleeding in hepatectomy is a challenge for surgeons. The hepatic pedicle clamping is a surgical maneuver that can provide reduction in bleeding, but it provokes a hepatocellular suffering. This, along with reperfusion after the clamping finishes, leads to an injury known as ischemia/reperfusion injury. Aim -To examine the effects of the ischemia/reperfusion injury on the liver after continuous and intermittent hepatic pedicle clamping in an animal model, using the quantification of apoptosis for evaluation. Method -Twenty New Zealand rabbits were assigned to groups 1 (control), 2 (60 minutes of continuous ischemia) and 3 (60 minutes of intermittent ischemia alternating 12 minutes of ischemia and three minutes of reperfusion). Liver biopsies were collected before ischemia, at its end and after six hours of reperfusion, when the animals were killed. The liver fragments were subjected to histological analysis (paraffinization and hematoxilin-eosin staining) and histochemical (Tunel reaction). Microscope fields of view were scanned for characterization and quantification of apoptosis. Results -Ischemia led to an increased apoptotic index in both experimental groups in comparison to controls, but similarly between them. After the reperfusion, the indexes returned to baseline values. Conclusion -Clamping of the hepatic pedicle, either continuous or intermittent, induces apoptosis in liver cells in a similar way. ABCDDV/852RESUMO -Racional -O controle do sangramento na hepatectomia é um desafio para os cirurgiões. A clampagem do pedículo hepático é manobra cirúrgica que pode promover redução do sangramento, mas provoca isquemia hepatocelular. Isso, junto com a reperfusão depois que a clampagem termina, leva à lesão de isquemia e reperfusão. Objetivo -Examinar os efeitos da lesão de isquemia e reperfusão no fígado após clampagem contínua e intermitente do pedículo hepático, usando a quantificação de apoptose como ferramenta. Método -Vinte coelhos New Zealand foram divididos em grupos 1 (controle), 2 (60 minutos de isquemia contínua) e 3 (60 minutos de isquemia intermitente alternando 12 minutos de isquemia e três minutos de reperfusão). Biópsias hepáticas foram colhidas antes e ao fim da isquemia e após seis horas de reperfusão, quando os animais eram sacrificados. Os fragmentos obtidos foram submetidos à análise histológica e histoquímica (reação de Tunel). Campos microscópicos foram analisados para caracterização e quantificação de apoptose. Resultados -A isquemia levou à elevação do índice apoptótico em ambos os grupos experimentais em relação aos controles, mas similar entre eles. Depois da reperfusão os índices voltaram aos valores iniciais. Conclusão -A clampagem do pedículo hepático, tanto contínua quanto intermitente, induz a apoptose em células hepáticas de modo igual.

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A model to study total hepatic ischemia–reperfusion injury

Cited by 27 publications

References 10 publications

Measurement of pentane in expiratory gas during rabbit hepatic ischemia/reperfusion by solid-phase microextraction and gas chromatography–mass spectrometry (SPME GC/MS)

Measurement of pentane in expiratory gas during rabbit hepatic ischemia/reperfusion by solid-phase microextraction and gas chromatography–mass spectrometry (SPME GC/MS)

Species differences in transgene DNA uptake in hepatocytes after adenoviral transfer correlate with the size of endothelial fenestrae

Lesão de isquemia e reperfusão após clampagem contínua ou intermitente do pedículo hepático em coelhos

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