A model to evaluate the biological effect of natural products: vincristine action on the biodistribution of radiopharmaceuticals in balb/c female mice

Mattos, D. M. M.; Gomes, M. L.; Freitas, Renata de; Rodrigues, Paula; Paula, Emílio F.; Bernardo-Filho, Mário

doi:10.1002/(sici)1099-1263(199907/08)19:4<251::aid-jat575>3.0.co;2-y

Cited by 14 publications

(5 citation statements)

References 4 publications

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“…Este alcaloide possui forte interação medicamentosa entre radiobiocomplexo, promovendo aumento da captação do radiobiocomplexo 99mTc-DTPA (ácido dietileno) pelo timo, ovário, útero, baço, rins, coração, estômago, pulmão, fígado e ossos (Brito et al, 1998). A vincristina também é capaz de alterar a biodisponibilidade do 99mTc-fitato em camundongos Balb/c (Mattos et al, 1999a); diminuir a absorção de 99mTc-MDP (ácido metilenodifosfónico) pelo útero, ovário, baço, timo, nódulos linfáticos (inguinal e mesentheric), rim, fígado, pâncreas, estômago, coração, cérebro e osso in vivo (Mattos et al, 1999b); reduzir a absorção de 99mTc-PYP (pirofosfato de sódio) pelo baço, timo, nódulos linfáticos (inguinal e mesentérico), rim, pulmão, fígado, pâncreas, estômago, coração e cérebro, porém aumentando a absorção do tecido ósseo e da tiróide em animais (Mattos et al, 1999b) e, por fim, diminuir a absorção do 99mTc-GHA (ácido glucoheptónico) pelo útero, ovário , baço, timo, nódulos linfáticos (inguinal e mesentérico), do rim e do coração (Mattos et al, 2001).…”

Section: Discussionunclassified

Efeito da fração não-oleosa doRicinus communisna biodistribuição comTecnécio-99m em camundongos

Mousinho

Rocha

Pereira

et al. 2015

Rev. bras. plantas med.

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RESUMO:Ricinus communis é uma planta pertence à família Euforbiaceae. A partir de seus frutos é extraído o óleo de rícino e outra fração altamente tóxica (fração não-oleosa). Neste estudo, foi avaliado o efeito da fração não oleosa de R. communis (FNORC) na biodistribuição do Tc-99m em camundongos sadios e em animais transplantados com Sarcoma-180 (S180). Para avaliação da biodistribuição do Tc-99m, o percentual de atividade radioativa total injetada (ATI%) foi mensurada utilizando um contador gama. Os resultados mostraram que, em animais sadios, houve aumento da captação do Tc-99m após o tratamento com FNORC pelos rins, cérebro e estômago. Apenas houve diminuição da absorção deste radioisótopo nos músculos. Em animais com tumor, houve redução significativa da captação do Tc-99m no coração, pulmões e tumor, e foi observado o aumento da captação apenas nos rins. Foi observada também a diminuição da concentração das proteínas plasmáticas totais em animais com tumor após tratamento com FNORC e atividade antitumoral significativa. Diante desses dados, conclui-se que fração não oleosa de R. communis possui atividade antitumoral significativa, porém altera a biodistribuição do Tc-99m, fato este que pode resultar em falhas nos processos de diagnóstico por imagem que utilizem este radioisótopo como marcador. Palavras-chave:Ricinus communis, Tecnécio-99m, Biodistribuição, Proteínas totais, Atividade antitumoral. ABSTRACT: Effect of non-oily fraction of Ricinus communis on the bio distribution withTechnetium-99m in mice. Ricinus communis is a plant belonging to the Euforbiaceae family. From its fruits, it is extracted the castor oil and another highly toxic fraction (non-oily fraction). In this study, we evaluated the effect of the non-oily fraction of R. communis (NOFRC) on the bio distribution of Tc-99m in healthy mice and in animals transplanted with Sarcoma-180 (S180). To evaluate the bio distribution of theTc-99m, the percentage of the total injected radioactivity (% TIR) was measured through a gamma counter. There was an increase in the capitation of Tc-99m after the treatment with NOFRC in the kidneys, brain, and stomach of healthy animals. A decrease in the reception of this radionuclide was only found in the muscles. In animals with tumour, there was a significant reduction in the uptake of Tc-99m in the heart, lungs and tumour. An increase in the capitation was only observed in the kidneys. It was also observed a reduction in the total plasma protein concentration in animals with tumours after the treatment with NOFRC, besides significant antitumor activity. We concluded that there is antitumor activity in the NOFRC, but that it alters the biodistribution of Tc-99m, a fact which may result in failures in the diagnostic imaging process using this radioisotope as a marker.

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Section: Discussionunclassified

Efeito da fração não-oleosa doRicinus communisna biodistribuição comTecnécio-99m em camundongos

Mousinho

Rocha

Pereira

et al. 2015

Rev. bras. plantas med.

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“…We are using two models, one based in the effect of drugs on the biodistribution of radiopharmaceuticals (Mattos et al, 1999) and other, concerning to the infl uence of drugs, mainly extracts of medicinal plants on the labeling of the blood constituents (Reineger et al, 1999;Oliveira et al, 2000. Here, we have studied the effect of the Pfaffi a sp.…”

Section: Discussionmentioning

confidence: 99%

An aqueous extract of Pfaffia sp. does not alter the labeling of blood constituents with technetium-99m and the morphology of the red blood cells

Fernandes¹,

Brito²,

Frydman³

et al. 2005

Rev. bras. farmacogn.

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ABSTRACT:Plants have been used for the human beings as food, as additives and/or as medicines. There are controversies about the biological effects of several natural products and, it is worthwhile to try to develop experimental assays to evaluate properties of extracts of plants. Pfaffi a sp. is utilized in popular medicine and various properties have been attributed to its extract. Red blood cells (RBC) and plasma proteins are labeled with technetium-99m (Tc-99m) and this labeling procedure depends on a reducing agent and stannous ion is usually used. There are reports that drugs can alter the labeling of blood elements with Tc-99m. We have evaluated the infl uence of a Pfaffi a sp. extract on the labeling of blood constituents with Tc-99m and on the morphology of RBC. Blood was incubated with an aqueous extract of Pfaffi a sp., stannous chloride and Tc-99m. Samples were centrifuged and plasma and blood cells were separated and also precipitated with trichloroacetic acid. Soluble and insoluble fractions were separated. The results did not show alteration in the uptake of radioactivity and no modifi cations on the shape of the RBC in presence of Pfaffi a sp. Once this labeling process depends on a reducing agent, probably, this extract has compounds with anti-oxidant properties as already described elsewhere, that could protect the stannous ions against the oxidation process. This fact would aid the labeling process of blood elements with Tc-99m.

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“…Moreover, other authors have suggested that medicinal plants (natural drugs) can be also associated with the DIR (Gomes et al, 2002a, Santos-Filho et al, 2004a, Moreno et al, 2004. Vincristine is a chemotherapeutic drug used in several protocols in oncology and (i) in an animal model, this drug has increased significantly the uptake of the radiobiocomplex 99m Tc-DTPA (diethylenetriaminepentaacetic acid) by the thymus, ovary, uterus, spleen, kidneys, heart, stomach, lungs, liver and bone (Britto et al, 1998), (ii) it modified the bioavailability of the 99m Tc-phytate in Balb/c mice (Mattos et al, 1999a), (iii) it decreased the uptake of 99m Tc-MDP (methylenediphosphonic acid) by the uterus, ovary, spleen, thymus, lymph nodes (inguinal and mesentheric), kidney, liver, pancreas, stomach, heart, brain and bone isolated from animals (Mattos et al, 1999b), (iv) it decreased the uptake of 99m Tc-PYP (sodium pyrophosphate) by the spleen, thymus, lymph nodes (inguinal and mesentheric), kidney, lung, liver, pancreas, stomach, heart and brain, and it increased the uptake by the bone and thyroid in the treated animals (Mattos et al, 1999b), (v) it decreased the uptake of the 99m Tc-GHA (glucoheptonic acid) by the uterus, ovary, spleen, thymus, lymph nodes (inguinal and mesentheric), kidney and heart isolated from animals (Mattos et al, 2001), and (vi) it increased the blood pool of the radioactivity of Ga-67 citrate (Hladik III et al, 1987). In experiments with mice, mitomycin-C (i) increased the uptake of 99m Tc-MDP in thymus, ovary, uterus, heart, stomach, pancreas, kidneys, spleen and lungs (Gomes et al, 1998), (ii) altered the uptake of radiobiocomplexes used for renal evaluations (Gomes et al, 2001), (iii) modified the bioavailability of 99m Tc-PYP (Gomes et al, 2002b) and (iv) interfered in the bioavailability of the 99m Tc-phytic acid (Gomes et al, 2002c) Buchsbaum et al, 1992 have reported PET studies of drug interaction with brain regional glucose metabolism.…”

Section: Undesirable Drug Interaction and The Bioavailability Of Radimentioning

confidence: 99%

Drug interaction with radiopharmaceuticals: a review

Bernardo-Filho

Santos-Filho

Moura

et al. 2005

Braz. arch. biol. technol.

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Clinical images are worthwhile in Health Sciences and their analysis and correct interpretation aid the professionals,such as physicians, physiotherapists and occupational therapists, to make decisions and take subsequent therapeutic and/or rehabilitation measures. Other factors, besides the state of the disease, may interfere and affect the bioavailability of the radiopharmaceuticals (radiobiocomplexes) and the quality of the SPECT and PET images. Furthermore, the labeling of some of these radiobiocomplexes, such as plasma proteins, white blood cells and red blood cells, with 99mT, can also be modified. These factors include drugs (synthetic and natural) and dietary conditions, as well as some medical procedures (invasive or non-invasive), such as radiation therapy, surgical procedures, prostheses, cardioversion, intubation, chemoperfusion, external massage, immunotherapy, blood transfusion and hemodialysis. In conclusion, the knowledge about these factors capable of interfering with the bioavailability of the radiobiocomplexes is worthwhile for secure diagnosis. Moreover, the development of biological models to study these phenomena is highly relevant and desirable.
Imagens clínicas são valiosas em Ciências da Saúde e a análise e a interpretação correta das mesmas auxiliam os profissionais, como médico, fisioterapeuta, terapeuta ocupacional, na tomada de decisões e subseqüentes ações terapêuticas e/ou de reabilitação. Além das doenças outros fatores podem interferir e afetar a biodisponibilidade dos radiofármacos (radiobiocomplexos) e a qualidade das imagens (SPECT e PET). Além disso, a marcação de alguns desses radiobiocomplexos com Tc-99m, como proteínas plasmáticas, leucócitos e hemácias, também pode ser modificada. Entre esses fatores, estão drogas (sintéticas e naturais) e condições alimentares, assim como alguns procedimentos médicos (invasivos e não invasivos), como a radioterapia, processos cirúrgicos, próteses, cardioversão, intubação, quimioperfusão, massagem externa, imunoterapia, transfusão sanguínea e hemodiálise. Em conclusão, o conhecimento sobre esses efeitos capazes de interferir na biodisponibilidade de radiobiocomplexos é altamente relevante para diagnósticos precisos. Além disso, o desenvolvimento de modelos biológicos para estudar esses fenômenos é de elevada importância e desejável

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A model to evaluate the biological effect of natural products: vincristine action on the biodistribution of radiopharmaceuticals in balb/c female mice

Cited by 14 publications

References 4 publications

Efeito da fração não-oleosa doRicinus communisna biodistribuição comTecnécio-99m em camundongos

Efeito da fração não-oleosa doRicinus communisna biodistribuição comTecnécio-99m em camundongos

An aqueous extract of Pfaffia sp. does not alter the labeling of blood constituents with technetium-99m and the morphology of the red blood cells

Drug interaction with radiopharmaceuticals: a review

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