A Model of Spontaneous Periodontitis in the Miniature Goat

Suzuki, Sachiyo; Mitani, Akio; Koyasu, Kazuhiro; Oda, Sen-ichi; Yoshinari, Nobuo; Fukuda, Mitsuo; Hanamura, Hajime; Nakagaki, Haruo; Noguchi, Toshihide

doi:10.1902/jop.2006.050203

Cited by 13 publications

(8 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With this process, from suppurative alveolar periostitis, the teeth roots become exposed, and teeth loosen. Our results and literature reveal that ovine periodontitis pathogenesis has similarities to miniature goat and cattle periodontitis (Döbereiner et al 1974, Suzuki et al 2006, Silva et al 2016.…”

Section: Discussionsupporting

confidence: 72%

“…Histological changes of spontaneous periodontitis in miniature goats included gingival epithelial hyperplasia and food accumulation between teeth (Suzuki et al 2006). Döbereiner et al (1974) characterized cattle periodontitis, including changes to the epithelium, forming small periodontal pockets, firstly without apparent inflammation, followed by accumulation of ingesta and sequent pocket enlargement.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Pathology of chronic ovine periodontitis

et al. 2022

View full text Add to dashboard Cite

Periodontitis is an inflammatory process of infectious origin affecting the teeth and their supporting structures, causing significant economic losses and reducing animal welfare. Bacteria in the gingival biofilm are one of the main factors in initiating inflammatory lesions. Bacteria act directly on tissues or indirectly through substances that cause tissue damage. Studies on the etiopathogenesis of periodontitis in Brazilian sheep herds are scarce. The present study aimed to characterize histologically periodontal lesions of culled sheep from the Brazilian breed, Santa Inês. Periodontal lesions, such as periodontal pockets containing plant tissue and bacteria, replacement of the periodontal ligament by connective tissue and inflammatory cells, superficial pustules, hydropic epithelial degeneration, and epithelium hyperplasia, were observed. Submucosal changes were characterized by granulation tissue, edema, swelling of the endothelial cells, bacteria, and predominantly perivascular lymphoplasmacytic inflammatory infiltrate. In the alveolar bone, osteoclastic resorption and bone apposition were observed. This study revealed subacute to chronic inflammation, alveolar bone resorption, and cortical bone apposition in ovine periodontitis. Thus, these findings can contribute to the evolution of knowledge about the etiopathogenesis of ovine periodontitis and, possibly, the development of measures to control the disease.

show abstract

Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Pathology of chronic ovine periodontitis

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Experimental periodontitis is a suitable model for exploring anti-inflammatory, osteogenic, and anti-osteoclast characteristics of biomaterials in bone regeneration, as it is easy to create and is accessible for observation. The application of animal models has been limited by several factors, such as small oral cavity size, operative difficulty, and expense (Suzuki et al, 2006). Mice are the most ideal animal models for pathological and therapeutic research of periodontitis, because the mouse-derived antigen of the experimental specimen FIGURE 2 | Orthodontic ligature wire insertion induces progressive alveolar bone loss.…”

Section: Discussionmentioning

confidence: 99%

A Simplified and Effective Method for Generation of Experimental Murine Periodontitis Model

Feng

Tang

et al. 2020

Front. Bioeng. Biotechnol.

View full text Add to dashboard Cite

Periodontitis, a common disease that can lead to bone destruction, periodontal attachment loss, and tooth loss, is the major cause for oral tissue engineering. Experimental periodontitis is a suitable disease-model for studying bone regeneration and the potential therapeutic role of biomaterials on periodontal tissue engineering, as this in vivo model could be employed to mimic the natural host response under bacteria-caused oral pathological environments. Although large animals with ligature-induced periodontitis have mostly been used for experiments, a mouse model is a better choice for several reasons. Inserting ligature threads through the interproximal space between the teeth is the key step in establishing a periodontitis model, and it is easy to achieve in large animals, but difficult in mice due to the limited operating space. In this work, we provide a new and proven approach for periodontitis induction in mice using C+ nickel-titanium root canal files and stainless-steel ligature wires. The validity of this method was assessed by evaluating alveolar bone loss via micro-CT and detecting periodontal inflammation by histological staining and qPCR after the treatments. Progressive alveolar bone loss was observed from day 3 after the ligature-placement. Infiltration and accumulation of F4/80+ macrophage was also detected. In accordance with the histological results, there was upregulation of the expression levels of the inflammatory genes Il1β, Tnf-α, and Il6 in gingival tissues isolated from the ligation sites. Our results suggest that this novel method could resolve the difficulty of ligature-placement in mice and consequently contribute to further use of mouse models for studying the pathological mechanisms of periodontitis and developing potential periodontal tissue regeneration strategies. C+ files, which are made of nickel-titanium, are tough, elastic, and sufficiently thin to pass through the interproximal space between the teeth after pre-bending to form an appropriate angle, thus providing an access for ligature wire insertion. As a common tool in the dental clinic, it is familiar to researchers of oral biology, and can provide the feasibility for wide application of our method.

show abstract

“…nucleatum have been identified in sheep with 'broken mouth' periodontitis (McCourtie et al, 1989) and F. necrophorum has been identified in goats with periodontitis (Suzuki et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Microbiomes associated with bovine periodontitis and oral health

Borsanelli

Lappin

Viora

et al. 2018

Veterinary Microbiology

View full text Add to dashboard Cite

Periodontitis is an infectious polymicrobial, immuno-inflammatory disease of multifactorial aetiology that has an impact on the health, production and welfare of ruminants. The objective of the present study was to determine the microbial profiles present in the gingival sulcus of cattle considered periodontally healthy and in the periodontal pocket of animals with periodontitis lesions using high-throughput bacterial 16S rRNA gene sequencing. Subgingival biofilm samples were collected from 40 cattle with periodontitis and 38 periodontally healthy animals. In total, 1923 OTUs were identified and classified into 395 genera or higher taxa. Microbial profiles in health differed significantly from periodontitis in their composition (p < 0.0001, F = 5.30; PERMANOVA) but no statistically significant differences were observed in the diversity of healthy and periodontitis microbiomes. The most prevalent taxa in health were Pseudomonas, Burkholderia and Actinobacteria, whereas in disease these were Prevotella, Fusobacterium and Porphyromonas. The most discriminative taxa in health were Gastranaerophilales, Planifilum and Burkholderia, and in disease these were Elusimicrobia, Synergistes and Propionivibrio. In conclusion, statistically significant difference exists between the microbiome in bovine oral health and periodontitis, with populations showing 72.6% dissimilarity. The diversity of the bacteria found in health and periodontitis were similar and bacteria recognised as periodontal pathogens showed increased abundance in disease. In this context, the main components of bacterial homeostasis in the biofilm of healthy sites and of dysbiosis in periodontal lesions provide unprecedented indicators for the evolution of knowledge about bovine periodontitis.

show abstract

A Model of Spontaneous Periodontitis in the Miniature Goat

Cited by 13 publications

References 37 publications

Pathology of chronic ovine periodontitis

Pathology of chronic ovine periodontitis

A Simplified and Effective Method for Generation of Experimental Murine Periodontitis Model

Microbiomes associated with bovine periodontitis and oral health

Contact Info

Product

Resources

About