A model of human nasal epithelial cells adapted for direct and repeated exposure to airborne pollutants

Bardet, Gaëlle; Achard, Sophie; Loret, Thomas; Desauziers, Valérie; Momas, Isabelle; Seta, Nathalie

doi:10.1016/j.toxlet.2014.05.023

Cited by 23 publications

(16 citation statements)

References 40 publications

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“…We hypothesized that after 3 days of exposure we could observe regulation of genes that support adaptation and/or defense against formaldehyde-mediated insults, while other studies using comparable concentrations but shorter exposure times were not able to detect cellular responses. Low environmental concentrations of approximately 0.04 ppm formaldehyde applied for 30 minutes did not increase the production of inflammation markers chemokine (C-C motif) ligand 2 (CCL2) and interleukin 8 (IL8) in A549 and BEAS-2B without any additional sensitization42 and also in another study, exposure to 0.2 ppm formaldehyde for 1 hour had no effect on IL-8 in A549 cells20. In a repeated exposure experiment of up to three exposures of epithelial cells of nasal origin for 1 hour to concentrations of approximately 0.17 ppm formaldehyde in 24 h intervals, no impact of treatment on cell viability and unchanged levels of IL-6 was reported by Bardet et al ., and only after the third treatment IL-8 levels were reduced compared to air exposed control cells43.…”

Section: Discussionmentioning

confidence: 98%

Cellular reactions to long-term volatile organic compound (VOC) exposures

Gostner

Zeisler

Alam

et al. 2016

Sci Rep

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Investigations of cellular processes initiated by volatile organic compounds (VOCs) are limited when modelling realistic long-term exposure scenarios at low concentrations. Exposure to indoor VOCs is associated with a range of adverse effects, but data on molecular changes at regulatory threshold limits are lacking. Activity analysis of VOC in vitro can be a valuable complement to inhalation toxicological evaluations. We developed an exposure platform that generates a stable VOC atmosphere and allows the exposure of cells for longer periods. Using formaldehyde as a model analyte, air-liquid interface cultured A549 lung epithelial cells were exposed to critical concentrations of 0.1 and 0.5 ppm for 3 days. Owing to the lack of known exposure biomarkers, we applied a genome-wide transcriptional analysis to investigate cellular responses at these sublethal concentrations. We demonstrate a minor overlap of differentially expressed transcripts for both treatment concentrations, which can be further analyzed for their use as exposure biomarkers. Moreover, distinct expression patterns emerge for 0.1 and 0.5 ppm formaldehyde exposure, which is reflected in significant enrichment of distinct biological processes. More specifically, metabolism of specific compound classes, lipid biosynthesis and lung-associated functions are affected by lower exposure levels and processes affecting proliferation and apoptosis dominate the higher exposure levels.

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Section: Discussionmentioning

confidence: 98%

Cellular reactions to long-term volatile organic compound (VOC) exposures

Gostner

Zeisler

Alam

et al. 2016

Sci Rep

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“…In vivo experiments have been designed to investigate the production of cytokines after FA exposure [ 13 , 19 , 35 , 36 ] because in vivo experiments are satisfactory simulations of real FA exposure situations. Recently, a few in vitro studies [ 21 , 22 , 23 , 24 ] have successfully created FA exposure conditions in specific cellular models at the air-liquid interface. However, different cell lines respond differently to FA exposure when sensitized with a macrophage secretion medium.…”

Section: Discussionmentioning

confidence: 99%

“…However, Ohtsuka et al showed an adverse result, as follows: both the mRNAs of Th1-related cytokines (IFN-γ and IL-2) and Th2-related cytokines (IL-4 and IL-5) showed the tendency to be depressed in BN and F344 rats after inhaling 1% FA aerosol for 5 d [ 13 ]. Moreover, the production of cytokines after FA exposure was investigated in in vitro studies besides in vivo studies [ 21 , 22 , 23 , 24 ]. On average, a 16.9-fold increase of the inflammatory response protein IL-8 was observed in human lung epithelial cells after exposure to FA at 1 ppm for 4 h [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

“…On average, a 16.9-fold increase of the inflammatory response protein IL-8 was observed in human lung epithelial cells after exposure to FA at 1 ppm for 4 h [ 24 ]. However, IL-6 production remained unchanged after exposure to FA at 200 μg/m 3 compared with air exposure [ 23 ]. Therefore, the effect of FA exposure on the secretion of Th1/Th2/Th17-related cytokines in serum remains controversial.…”

Section: Introductionmentioning

confidence: 99%

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Aberrant Production of Th1/Th2/Th17-Related Cytokines in Serum of C57BL/6 Mice after Short-Term Formaldehyde Exposure

Wei

Tan

Sun

et al. 2014

IJERPH

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Previous studies have shown that formaldehyde (FA) could cause immunotoxicity by changing the number of T lymphocytes and that cytokines play a pivotal role in the regulation of T lymphocytes. However, the previously used cytokine detection methods are difficult to use in the measurement of several cytokines in a small amount of sample for one test. Therefore, the cytometric bead array (CBA) technique was used. CBA showed better analytical efficiency and sensitivity than the previous methods. C57BL/6 mice were exposed to the control (normal saline), low FA concentration (0.5 mg/kg), and high FA concentration (2 mg/kg) for 1 week or 1 month. The contents of cytokines, including Th1-related cytokines (IL-2, IFN-γ, and tumor necrosis factor), Th2-related cytokines (IL-4, IL-6, and IL-10), and Th17-related cytokines (IL-17A), were measured by using the BD FACS Canto II Flow Cytometer and analyzed by FCAP ArrayTM Software. Th1/Th2/Th17-related cytokines showed a slightly decreasing trend after low FA exposure. Conversely, a significantly increasing trend was found after high FA exposure. Th1/Th2/Th17-related cytokines all serve important functions in the immune reactions in mice after FA exposure.

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“…To quantify the oxidative potential of airborne particulate matter, both in-vivo and in-vitro studies have been conducted. These studies have found correlations between the oxidative potential of airborne particles and the production of biological markers of ROS formation and oxidative stress (Bardet et al, 2014;Hawley et al, 2014;Li et al, 2004;Swanson et al, 2009;Acworth et al, 1999;Kim et al, 2001;Maier et al, 2008;Oberdorster, 2000). Yet the extent of these measurements is limited.…”

Section: Introductionmentioning

confidence: 99%

An On-line Monitor of the Oxidative Capacity of Aerosols (o-MOCA)

Eiguren-Fernandez¹,

Kreisberg²,

Hering³

2016

Preprint

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Abstract. The capacity of airborne particulate matter to generate reactive oxygen species (ROS) has been correlated with the generation of oxidative stress both in-vitro and in-vivo. The cellular damage from oxidative stress, and by implication with ROS, is associated with several common diseases such as asthma, chronic obstructive pulmonary disease (COPD), and some neurological diseases. Yet currently available chemical and in-vitro assays to determine the oxidative capacity of ambient particles require large samples, analysis are typically done offline, and the results are not immediate. In this manuscript we report the development of an on-line monitor of the oxidative capacity of aerosols (o-MOCA) to provide on-line, time-resolved assessment of the capacity of airborne particles to generate ROS. Our approach combines the Liquid Spot Sampler (LSS), which collects particles directly into small volumes of liquid, and a chemical module optimized for on-line measurement of the oxidative capacity of aerosol using the dithiothreitol (DTT) assay. The LSS uses a three-stage, laminar-flow water condensation approach to enable the collection of particles as small as 5 nm into liquid, without subjecting the sample to temperature extremes. The DTT assay has been improved to allow the on-line, time-resolved analysis of samples collected with the LSS. The o-MOCA was optimized and its performance evaluated using the 9,10-Phenanthraquinone (PQ) as standard redox-active compound. Laboratory testing shows minimum interferences or carry-over between consecutive samples, low blanks, and a reproducible, linear response between the DTT consumption rate (nmol/min) and PQ concentration (µM). The calculated limit of detection for o-MOCA was 0.15 nmol/min. The system was validated with a Diesel Exhaust Particle (DEP) extract, previously characterized and used for the development, improvement, and validation of the standard DTT analysis. The DTT consumption rates (nmol/min) obtained with the o-MOCA were within experimental uncertainties of those previously reported for these DEP samples. In ambient air testing, the fully automated o-MOCA was run unattended for 3 days with 3-h time resolution, and showed a diurnal and daily variability in the measured consumption rates (nmol/min/m3).

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A model of human nasal epithelial cells adapted for direct and repeated exposure to airborne pollutants

Cited by 23 publications

References 40 publications

Cellular reactions to long-term volatile organic compound (VOC) exposures

Cellular reactions to long-term volatile organic compound (VOC) exposures

Aberrant Production of Th1/Th2/Th17-Related Cytokines in Serum of C57BL/6 Mice after Short-Term Formaldehyde Exposure

An On-line Monitor of the Oxidative Capacity of Aerosols (o-MOCA)

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