A model kidney transplant studied by phosphorus nuclear magnetic resonance

Sehr, Peter; Radda, George K.; Bore, Peter J.; Sells, R. A.

doi:10.1016/s0006-291x(77)80182-4

Cited by 77 publications

(22 citation statements)

References 10 publications

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“…High-resolution 31p nuclear magnetic resonance (NMR) spectroscopy can be used to investigate and monitor molecular events occurring within intact, living cells. This noninvasive, nondestructive method allows the simultaneous and continuous detection of intracellular phosphate compounds such as ATP, phosphocreatine, Pi, and 2,3-diphosphoglycerate and has proved valuable in the study of a variety of cell and organ preparations including bacteria (1,2), yeast (3), erythrocytes (4,5), skeletal muscle (6, 7), brain (8), liver (9), kidney (10), and heart (11)(12)(13). The application of 31P NMR to the detection of phosphate compounds in intact, living animals was first attempted by Chance et al: (8), who measured the 31p NMR spectrum of the head of a mouse.…”

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confidence: 99%

Analysis of rat heart in vivo by phosphorus nuclear magnetic resonance.

Grove¹,

Ackerman²,

Radda³

et al. 1980

Proc. Natl. Acad. Sci. U.S.A.

109

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High-resolution 31P nuclear magnetic resonance spectra at 73.83 MHz are reported for rat heart in vivo. In live rats, it was possible to observe the cardiac content of ATP, phosphocreatine,.and Pi. Only a small amount of wholeblood 2,3-diphosphoglycerate was observed in the spectra, precluding the possibility that blood phosphate compounds were masking the spectra of cardiac phosphate compounds. The 31p nuclear magnetic resonance spectra of in vivo and perfused rat hearts were similar and sup ort the utilization of the perfused rat heart as a model system for studying high-energy phosphate metabolism of the heart in vivo. The dynamic flux of highenergy phosphate compounds was investigated by subjecting the rat to respiratory arrest. In this experiment, the heart followed the classic metabolic pattern known to occur during cardiac arrest; phosphocreatine and then ATP decreased in concentration while Pi increased in concentration. The 31p nuclear magnetic resonance analysis of rat heart in vivo is demonstrated to be a practical and feasible method for studying cardiac high-energy phosphate metabolism.Much information has been gained from the in vitro study of isolated biochemical reactions and physiological functions. However, the ultimate description of these processes must be in terms of their regulation and integration within the living organism. High-resolution 31p nuclear magnetic resonance (NMR) spectroscopy can be used to investigate and monitor molecular events occurring within intact, living cells. This noninvasive, nondestructive method allows the simultaneous and continuous detection of intracellular phosphate compounds such as ATP, phosphocreatine, Pi, and 2,3-diphosphoglycerate and has proved valuable in the study of a variety of cell and organ preparations including bacteria (1, 2), yeast (3), erythrocytes (4, 5), skeletal muscle (6, 7), brain (8), liver (9), kidney (10), and heart (11-13). The application of 31P NMR to the detection of phosphate compounds in intact, living animals was first attempted by Chance et al: (8), who measured the 31p NMR spectrum of the head of a mouse. We report here a different application in which we selectively placed the NMR receiver coil around the organ to be analyzed. Using this technique, we have successfully measured the intracellular level and dynamic flux of ATP and phosphocreatine in the hearts of live rats.MATERIALS AND METHODS Animals. Male Wistar rats (250-350 g) maintained on a standard diet were used throughout.Whole-Animal Procedure. Animals were anesthetized with intraperitoneal Sagatal (60 mg/kg of body weight; sodium pentobarbitone, 60 mg/ml). The trachea was intubated via a tracheostomy and the animal was mechanically ventilated 704-0 breaths per min with 50% nitrous oxide/50% oxygen. The abdomen was entered through an upper midline incision and the diaphragm was incised anterially. Access to the heart was completed by a lower median stenotomy. The animal was then secured in the NMR probe in a vertical position. The NMR receiver coil was intro...

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confidence: 99%

Analysis of rat heart in vivo by phosphorus nuclear magnetic resonance.

Grove¹,

Ackerman²,

Radda³

et al. 1980

Proc. Natl. Acad. Sci. U.S.A.

109

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“…The measurement o f in vivo metabolism using N M R spectroscopy in the intact organism as well as in isolated perfused organs such as kidneys has been documented in numerous studies [1][2][3][4][5][6][7]. N M R magnets available in the past were of limited inner diameter.…”

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confidence: 99%

Application of P-31 Nuclear Magnetic Resonance Spectroscopy to a New Experimental Kidney Perfusion Model Using Cadaveric Porcine Kidneys from Slaughterhouse

Lietzenmayer

Henze²,

Knorpp³

et al. 1991

Nephron

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Larger NMR magnets with relatively high field strengths have become available recently, allowing the application of magnetic resonance spectroscopy (MRS) in larger mammalian organs. The aim of this study was to develop and test a new and simple kidney perfusion model from slaughterhouse swine using a new 4.7-tesla/40-cm diameter system, with the intention behind to provide a human-like mammalian experimental kidney perfusion model, and to avoid sensitive in vivo animal experiments on higher-developed mammalians. 35 pig kidneys obtained 10–15 min post mortem were studied to evaluate and define conditions for optimum metabolic preservation with the following perfusion protocols: (1) immediate plegia with cold Collins solution, 1–3 h cold storage, P-31 MRS; (2) immediate plegia, 1–3 h cold storage, blood reperfusion, P-31 MRS; (3) immediate blood reperfusion, plegia, 1–3 h cold storage, blood reperfusion, P-31 MRS; (4) immediate blood reperfusion, plegia, 24 h cold storage, blood reperfusion, P-31 MRS. P-31 MRS at 81 MHz with a double-tuned surface coil yielded the following results: Protocol, No.

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“…The first NMR studies of renal tissue were the 3IP NMR studies of the effects of renal ischaemia on a rat kidney transplant model published by Sehr et al [1] in 1977. Despite this early start, progress in renal NMR studies was hindered by the bi ological complexity and physiological de mands of the intact in vitro kidney preparation compared with more homogeneous tissues such as heart and brain.…”

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