A Model for Arabidopsis Thaliana Cell Suspension Growth and Sugar Uptake Kinetics

Abstract

Arabidopsis thaliana (A. thaliana) is a small weed which is related to the cabbage and mustard family. This flowering plant has been used widely as a model plant in genetics and molecular biology research since it is the first plant the full sequenced genome. Thus, A. thaliana literature provides plentiful information from genomics and transcriptomics point of view. However, there is still a lack of physiological information regarding its cell suspension cultures which can be powerful research tools in Plant Biotechnology and especially in Plant Systems and Synthetic Biology. In this study, cell growth and sugar uptake of A. thaliana Col ecotype grown in the continuous dark condition were modelled using the modified Monod and Michelis-menten equations. The model included sucrose hydrolysis by the cell-wall invertase enzyme into hexoses (glucose and fructose) and consumption of these hexoses at different rates to support cell growth. All kinetic model parameters were obtained from a control experiment where Col cells were grown on 30 gL-1 sucrose as well as other independent experiments where Col cells were supplied with different concentrations and combinations of sugars. The model adequately described and predicted the growth and sugars profile of A. thaliana cells. This model can also be applied for larger scale of growth with extended expressions for oxygen uptake rate, carbon dioxide production rate etc.

Simulation and optimization of nutrient uptake and biomass formation using a multi-parameter Monod-type model of tobacco BY-2 cell suspension cultures in a stirred-tank bioreactor

“…The sucrose to glucose or fructose DM conversion parameters (m SG and m SF ) were set at 0.526 because one mole of sucrose always produces one mole each of glucose and fructose ( Puad et al., 2017 ). This model is defined hereafter as the “improved model”.…”

“…A mechanistic, kinetic modeling approach is preferred for PCCs, where all metabolic effects are lumped into unstructured kinetic functions for simplification ( Resat et al., 2009 ), especially for model-based DoE where the number of experiments is small and hence only few model parameters can be fitted. In particular, Monod-type models are preferred because they simulate nutrient uptake, biomass formation and protein production while differentiating between viable and dead cells ( Prakash and Srivastava, 2006 ; Prakash and Srivastava, 2008 ; Jiménez-Hornero et al., 2009a ; Jiménez-Hornero et al., 2009b ; Puad et al., 2017 ; Villegas et al., 2017 ; Puad and Abdullah, 2018 ; Jacob et al., 2020 ).…”

Plant Heterotrophic Cultures: No Food, No Growth

“…This was confirmed by modifications in the gene expression profiles [ 189 ]. The aging of plant cell cultures was accompanied by the exhaustion of sugars in the medium [ 190 , 191 ]. A decrease in sugar content is a common reaction to the carbohydrate starvation of cells in culture [ 192 , 193 , 194 , 195 ].…”