A Mobilização do Cálcio em Biomphalaria glabrata Exposta a Diferentes Quantidades de Carbonato de Cálcio

Magalhães, A. C.; Pinheiro, Jairo; Mello-Silva, Clélia Christina

doi:10.5216/rpt.v40i1.13916

Cited by 7 publications

(8 citation statements)

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“…In general, results from the present study indicated that the handmade shell-based substrate resulted in improved survival and growth rate, shell strength, and reproductive performance (short time of sexual maturation and increase in the rate of oviposition), which is comparable to results obtained from snails bred using the standardized substrate recommended by the Brazilian Ministry of Health. Improvements of B. glabrata physiological performance have already been demonstrated by several researchers, under laboratory conditions, when the aquatic environment in which they are bred has the presence of optimal levels of bioavailable calcium 10,12,18,19 . This demonstrates that B. glabrata shells can be an effective and low cost alternative to the calcium carbonate supplementation for the breeding of these snails in the laboratory.…”

Section: Discussionmentioning

confidence: 89%

Effectiveness of a handmade shell-based substrate for the breeding of Biomphalaria glabrata under laboratory conditions

Rodrigues

Lira

Nogueira

et al. 2020

Rev. Inst. Med. trop. S. Paulo

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Section: Discussionmentioning

confidence: 89%

Effectiveness of a handmade shell-based substrate for the breeding of Biomphalaria glabrata under laboratory conditions

Rodrigues

Lira

Nogueira

et al. 2020

Rev. Inst. Med. trop. S. Paulo

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“…In addition, the shells of Biomphalaria spp. participate directly in the regulation of calcium metabolism [ 28 ], which can also contribute to species differentiation.…”

Section: Resultsmentioning

confidence: 99%

“…tenagophila and the possible potential for hybridization [ 23 , 30 ]. Considering that the shells composition reflects the metabolic projections of the animal, it was expected that the three species could be differentiated using the shells alone, possibly associated with the chemical and protein arrangement of the organic matrix of the shells of these animals [ 26 , 28 , 31 ].…”

Section: Resultsmentioning

confidence: 99%

Feasibility of near-infrared spectroscopy for species identification and parasitological diagnosis of freshwater snails of the genus Biomphalaria (Planorbidae)

et al. 2021

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Near Infrared Spectroscopy (NIRS) has been applied in epidemiological surveillance studies of insect vectors of parasitic diseases, such as the Dengue’s mosquitoes. However, regarding mollusks, vectors of important worldwide helminth diseases such as schistosomiasis, fascioliasis and angiostrongyliasis, NIRS studies are rare. This work proposes to establish and standardize the procedure of data collection and analysis using NIRS applied to medical malacology, i.e., to mollusk vectors identifications. Biomphalaria shells and live snails were analyzed regarding several operational aspects, such as: moisture, shell side and position of the live animal for acquisition of NIR spectra. Representative spectra of Biomphalaria shells and live snails were collected using an average of 50 scans per sample and resolution of 16 cm-1. For shells, the sample should first be dried for a minimum of 15 days at an average temperature of 26±1°C, and then placed directly in the equipment measurement window with its left side facing the light beam. Live animals should be dried with absorbent paper; placed into a glass jar, and analyzed similarly to the shells. Once standardized, the technique was applied aiming at two objectives: identification of Biomphalaria using only the shells and parasitological diagnosis for Schistosoma mansoni infection. The discrimination of the three Biomphalaria species intermediate hosts of S. mansoni only by shell has technical limit due to the scarcity of organic material. Nevertheless, it was possible to differentiate B. straminea from B. tenagophila and B. glabrata with 96% accuracy. As for the parasitological diagnosis, it was possible to differentiate infected mollusks shedding S. mansoni cercariae from the non-infected ones with 82, 5% accuracy. In conclusion, the Near Infrared Spectroscopy (NIR’s) technique has proven to be an innovative and sound tool to detect infection by S. mansoni in the different species of Biomphalaria intermediate hosts.

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“…In our study, the exposure of B. glabrata to different concentrations of CaCO 3 caused no change in Ca 2+ concentration in the hemolymph, which suggests that this ion was transported via hemolymph to different reservoirs. Some of the possible reservoirs could be the shell (Marxen et al, 2003), the calcium cells present in the connective tissue of the snail (Davies and Erasmus, 1984), or it could be stored in the form of pearls in the digestive gland or intestine (Magalhães et al, 2011a). For this reason, the described increase in Ca 2+ in B. glabrata hemolymph when exposed to 20 mg/L CaCO 3 for fifteen days could be explained by the mobilization of this ion from the shell to the hemolymph to work as a buffering agent (Becker, 1980).…”

Section: Discussionmentioning

confidence: 99%

Changes in energetic metabolism of Biomphalaria glabrata (Mollusca, Planorbidae) in response to exogenous calcium

et al. 2016

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Calcium is considered an essential element for the metabolism of aquatic snail Biomphalaria glabrata (Say, 1818), intermediate host of Schistosoma mansoni Sambon, 1907 in Brazil, and represents a limiting factor to its distribution and adaptation to the environment. This study investigated the effect of different concentrations of exogenous CaCO 3 on the energetic metabolism of B. glabrata for better understanding the physiological interference of chemical elements dissolved in the environment with the physiology of this species. Sixty-day-old snails were distributed into six groups, five exposed to different concentrations of CaCO 3 (20, 40, 60, 80 and 100 mg/L) and a control group. The exposure to CaCO 3 was assessed over time, with analysis of 15 snails of each group in the following intervals: 1, 14, 21 or 30 days for hemolymph extraction. Concentrations of calcium and glucose in the hemolymph were determined by commercial kits, and organic acids were extracted using an ion exchange column and analyzed by high-performance liquid chromatography. Concentration of calcium in the hemolymph showed no significant difference (p>0.05) from the control group and between the concentrations tested. Concentration of glucose decreased (p<0.05) in the treatments of exposure to 20 and 40 mg/L and increased when exposed to 80 and 100 mg/L CaCO 3 compared to control and to other concentrations tested over 30 days. The organic acids pyruvate, oxaloacetate, citrate, succinate, fumarate, beta-hydroxybutyrate and lactate presented increased concentrations, while propionate and acetoacetate, decreased concentrations, when exposed to CaCO 3 compared to control. Considering the influence of different periods of exposure to CaCO 3 , on the 14 th day, there were stronger alterations in the metabolism of B. glabrata. In conclusion, exposure to CaCO 3 reduced the concentration of glucose, which is metabolized into pyruvate, the final product of glycolysis, and also influenced the energetic metabolism pathways, indicating an aerobic or partially anaerobic functioning.Keywords: snails, Krebs cycle, CaCO 3 exposure, energetic metabolism. (20, 40, 60, 80 e 100mg/L) e um controle. A exposição ao CaCO 3 foi avaliada em função do tempo, sendo retirados 15 moluscos de cada grupo nos seguintes intervalos: 1, 14, 21 ou 30 dias para extração da hemolinfa. As concentrações de cálcio e glicose na hemolinfa foram determinadas usando-se kits comercial e os ácidos orgânicos foram extraídos por meio da coluna de troca iônica e analisados através cromatografia líquida Alterações no metabolismo energético deBraz.

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A Mobilização do Cálcio em Biomphalaria glabrata Exposta a Diferentes Quantidades de Carbonato de Cálcio

Cited by 7 publications

References 1 publication

Effectiveness of a handmade shell-based substrate for the breeding of Biomphalaria glabrata under laboratory conditions

Effectiveness of a handmade shell-based substrate for the breeding of Biomphalaria glabrata under laboratory conditions

Feasibility of near-infrared spectroscopy for species identification and parasitological diagnosis of freshwater snails of the genus Biomphalaria (Planorbidae)

Changes in energetic metabolism of Biomphalaria glabrata (Mollusca, Planorbidae) in response to exogenous calcium

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