A Mitochondrion‐Specific Photoactivatable Fluorescence Turn‐On AIE‐Based Bioprobe for Localization Super‐Resolution Microscope

Gu, Xinggui; Zhao, Engui; Zhao, Teng; Kang, Miaomiao; Chen, Gui; Lam, Jacky W. Y.; Du, Shengwang; Loy, M. M. T.; Tang, Ben Zhong

doi:10.1002/adma.201505906

Cited by 175 publications

(152 citation statements)

References 44 publications

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“…The double bonds in these AIEgens may also lead to potential photobleaching or photooxidation which will result in a fluorescence loss. 20,21 As well as these drawbacks in AIEgens with double bonds, some shortcomings also exist in other propeller-like AIEgens. For example, silole and its derivatives, HPS and DMTPS, are usually hard to prepare and are unstable under alkaline conditions.…”

Section: Introductionmentioning

confidence: 99%

2,5-bis(4-alkoxycarbonylphenyl)-1,4-diaryl-1,4-dihydropyrrolo[3,2-b]pyrrole (AAPP) AIEgens: tunable RIR and TICT characteristics and their multifunctional applications

Liu

et al. 2017

Chem. Sci.

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162

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Section: Introductionmentioning

confidence: 99%

2,5-bis(4-alkoxycarbonylphenyl)-1,4-diaryl-1,4-dihydropyrrolo[3,2-b]pyrrole (AAPP) AIEgens: tunable RIR and TICT characteristics and their multifunctional applications

Liu

et al. 2017

Chem. Sci.

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162

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“…Subsequently, another fluorescence turn-on photoactivatable AIE mitoprobe of o-TPE-ON + with yellow emission was reported, [73] which could be photoactivated after transforming into c-TPE-ON + through the same mechanism as previous one ( Figure 4A). The probe could efficiently light up the mitochondria in living and fixed HeLa cells upon UV light irradiation.…”

Section: Super-resolution Imaging Of Mitochondria By Aie Mitoprobesmentioning

confidence: 61%

“…(D)Real-time monitoring of the mitochondrial dynamics in HeLa cells stained by o-TPE-ON + using super-resolution microscope [73]. (B) Photoactivation and photobleaching behaviors of mitochondria in fixed HeLa cells after incubation with o-TPE-ON + .…”

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confidence: 99%

Organic Mitoprobes based on Fluorogens with Aggregation‐Induced Emission

Cai

Feng

et al. 2018

Israel Journal of Chemistry

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Fluorescence imaging of mitochondria is of great interest to understand its functions and roles in various critical bioprocesses. Conventional fluorescence reagents for mitochondrial imaging and tracking suffer from various problems such as poor photostability and high cytotoxicity. The emerging of fluorogens with aggregation‐induced emission (AIE) property provides great opportunities to develop mitoprobes with high specificity, good photostability and multiple functions for mitochondrial imaging and tracking. This review summarizes the recent advance of AIE mitoprobes, including the design principle for AIE mitoprobes, the applications of AIE mitoprobes for super‐resolution mitochondrial imaging and tracking, as well as the therapeutic functions of AIE mitoprobes for cancer cell ablation.

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“…To solve the problem with ACQ effects, a group of AIEgens has been successfully designed for mitochondria targeting and imaging . An AIE‐based dye, TPE‐Ph‐In, which is a positively charged cationic molecule, could be simply synthesized by incorporating indolium salt into the TPE unit ( Figure ).…”

Section: Tpe‐ph‐inmentioning

confidence: 99%

The Biological Applications of Two Aggregation‐Induced Emission Luminogens

Zeng

Mai

et al. 2019

Biotechnology Journal

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Fluorescence imaging, as a commonly used scientific tool, is widely applied in various biomedical and material structures through visualization technology. Highly selective and sensitive luminescent biological probes, as well as those with good water solubility, are urgently needed for biomedical research. In contrast to the traditional aggregation‐caused quenching of fluorescence, in the unique phenomenon of aggregation‐induced emission (AIE), the individual luminogens have extremely weak or no emissivity because they each have free intramolecular motion; however, when they form aggregates, these components immediately “light up”. Since the discovery of “turn‐on” mechanism, researchers have been studying and applying AIE in a variety of fields to develop more sensitive, selective, and efficient strategies for the AIE dyes. There are numerous advantages to the use of AIE‐based methods, including low background interference, strong contrast, high performance in intracellular imaging, and the ability for long‐term monitoring in vivo. In this review, two typical examples of AIEgens, TPE‐Cy and TPE‐Ph‐In, are described, including their structure properties and applications. Recent progress in the biological applications is mainly focused on. Undoubtedly, in the near future, an increasing number of encouraging and practical ideas will promote the development of more AIEgens for broad use in biomedical applications.

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A Mitochondrion‐Specific Photoactivatable Fluorescence Turn‐On AIE‐Based Bioprobe for Localization Super‐Resolution Microscope

Cited by 175 publications

References 44 publications

2,5-bis(4-alkoxycarbonylphenyl)-1,4-diaryl-1,4-dihydropyrrolo[3,2-b]pyrrole (AAPP) AIEgens: tunable RIR and TICT characteristics and their multifunctional applications

2,5-bis(4-alkoxycarbonylphenyl)-1,4-diaryl-1,4-dihydropyrrolo[3,2-b]pyrrole (AAPP) AIEgens: tunable RIR and TICT characteristics and their multifunctional applications

Organic Mitoprobes based on Fluorogens with Aggregation‐Induced Emission

The Biological Applications of Two Aggregation‐Induced Emission Luminogens

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