A mitochondrial targeted two-photon iridium(III) phosphorescent probe for selective detection of hypochlorite in live cells and in vivo

Li, Guanying; Lin, Qian; Sun, Lingli; Feng, Changsheng; Zhang, Pingyu; Yu, Baoxian; Chen, Yu; Wen, Ya; Wang, Hui; Ji, Liang‐Nian; Chao, Hui

doi:10.1016/j.biomaterials.2015.02.106

Cited by 117 publications

(65 citation statements)

References 56 publications

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“…Over the measured range, the maximum TPA cross sections δ max of 1 a – 3 a and 1 b – 3 b ranged from 38 to 129 GM (1 GM=10 −50 cm 4 photon −1 ) at λ =740 nm (Figure S14 and Table S3 of the Supporting Information). These values are moderate compared with those of other Ir III complexes measured by the same method ,. From the log–log plot of the emission intensity against incident power, the linear regression slopes of 1 a – 3 a and 1 b – 3 b are 1.98±0.01, 2.00±0.03, 2.05±0.01, 1.95±0.02, 2.05±0.01 and 1.94±0.01, respectively, which indicate nonlinear TPA of the Ir III complexes (Figure S15 of the Supporting Information).…”

Section: Resultsmentioning

confidence: 82%

Valproic Acid‐Functionalized Cyclometalated Iridium(III) Complexes as Mitochondria‐Targeting Anticancer Agents

Chen

Tan

et al. 2017

Chemistry A European J

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Valproic acid (VPA) is a short-chain, fatty acid type histone deacetylase inhibitor (HDACi), which can cause growth arrest and induce differentiation of transformed cells. Phosphorescent cyclometalated Ir complexes have emerged as potential anticancer agents. By conjugation of VPA to Ir complexes through an ester bond, VPA-functionalized cyclometalated iridium(III) complexes 1 a-3 a were designed and synthesized. These complexes display excellent two-photon properties, which are favorable for live-cell imaging. The ester bonds in 1 a-3 a can be hydrolyzed quickly by esterase and display similar inhibition of HDAC activity to VPA. Notably, 1 a-3 a can overcome cisplatin resistance effectively and are about 54.5-89.7 times more cytotoxic than cisplatin against cisplatin-resistant human lung carcinoma (A549R) cells. Mechanistic studies indicate that 1 a-3 a can penetrate into human cervical carcinoma (HeLa) cells quickly and efficiently, accumulate in mitochondria, and induce a series of cell-death-related events mediated by mitochondria. This study gives insights into the design and anticancer mechanisms of multifunctional anticancer agents.

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Section: Resultsmentioning

confidence: 82%

Valproic Acid‐Functionalized Cyclometalated Iridium(III) Complexes as Mitochondria‐Targeting Anticancer Agents

Chen

Tan

et al. 2017

Chemistry A European J

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“…12c, 16 More importantly, Ir III complexes have been developed as two‐photon mitochondrial probes 16d. e In the present work, to obtain specific novel complexes for mitochondrial two‐photon phosphorescent probes, a series of 2‐phenylimidazo‐[4,5‐][1,10]phenanthroline derivatives that exhibited excellent light‐emitting efficiency were synthesized and conjugated to iridium(III). The cellular location of these Ir III complexes was determined using one‐photon microscopy (OPM) and two‐photon microscopy (TPM) imaging with MitoTracker Green FM (MTG) co‐staining and inductively coupled plasma mass spectrometry (ICP‐MS).…”

Section: Introductionmentioning

confidence: 99%

Cyclometalated Iridium(III) Complexes as Two‐Photon Phosphorescent Probes for Specific Mitochondrial Dynamics Tracking in Living Cells

Jin

Liu

Chen

et al. 2015

Chemistry A European J

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Five cyclometalated iridium(III) complexes with 2-phenylimidazo[4,5-f][1,10]phenanthroline derivatives (IrL1-IrL5) were synthesized and developed to image and track mitochondria in living cells under two-photon (750 nm) excitation, with two-photon absorption cross-sections of 48.8-65.5 GM at 750 nm. Confocal microscopy and inductive coupled plasma-mass spectrometry (ICP-MS) demonstrated that these complexes selectively accumulate in mitochondria within 5 min, without needing additional reagents for membrane permeabilization, or replacement of the culture medium. In addition, photobleaching experiments and luminescence measurements confirmed the photostability of these complexes under continuous laser irradiation and physiological pH resistance. Moreover, results using 3D multicellular spheroids demonstrate the proficiency of these two-photon luminescent complexes in deep penetration imaging. Two-photon excitation using such novel complexes of iridium(III) for exclusive visualization of mitochondria in living cells may substantially enhance practical applications of bioimaging and tracking.

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“…Additionally, composite biosensors that incorporate a sensor functionalised to a nanoparticle (gold particles, UCNP and QDots) are used to detect and/or measure ROS/RNS species (detailed in Table 1). Such ROS species probes can quantify hypochlorite [200,201], hydroxyl [202,203], superoxide [204], hydrogen peroxide [205] and singlet oxygen [206]. Biosensors for nitric oxide [207,208], nitroxyl [209–211], peroxynitrite [212] are also being developed.…”

Section: Figurementioning

confidence: 99%

Nitroxidative Signaling Mechanisms in Pathological Pain

Grace

Gaudet

Staikopoulos

et al. 2016

Trends in Neurosciences

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Tissue injury can initiate bidirectional signaling between neurons, glia and immune cells that creates and amplifies pain. While the ability for neurotransmitters, neuropeptides, and cytokines to initiate and maintain pain has been extensively studied, recent work has identified a key role for reactive oxygen and nitrogen species (nitroxidative species), including superoxide, peroxynitrite, and hydrogen peroxide. In this review, we describe how nitroxidative species are generated after tissue injury, and the mechanisms by which they enhance neuroexcitability in pain pathways. Finally, we discuss potential therapeutic strategies for normalizing nitroxidative signaling, which may also enhance opioid analgesia, to help to alleviate the enormous burden of pathological pain.

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A mitochondrial targeted two-photon iridium(III) phosphorescent probe for selective detection of hypochlorite in live cells and in vivo

Cited by 117 publications

References 56 publications

Valproic Acid‐Functionalized Cyclometalated Iridium(III) Complexes as Mitochondria‐Targeting Anticancer Agents

Valproic Acid‐Functionalized Cyclometalated Iridium(III) Complexes as Mitochondria‐Targeting Anticancer Agents

Cyclometalated Iridium(III) Complexes as Two‐Photon Phosphorescent Probes for Specific Mitochondrial Dynamics Tracking in Living Cells

Nitroxidative Signaling Mechanisms in Pathological Pain

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