A mitochondria-targeted chemiluminescent probe for detection of hydrogen sulfide in cancer cells, human serum and in vivo

Abstract

Hydrogen sulfide (H2S) as a critical messenger molecule plays vital roles in regular cell function. However, abnormal levels of H2S, especially mitochondrial H2S, is directly correlated with the formation of...

Hyper-Responsive Chemiluminescent Probe Reveals Distinct PYRase Activity in Pseudomonas aeruginosa

“…The incorporation of an acrylate substituent at the ortho position of a phenoxy-adamantyl-1,2-dioxetane, prevents water-mediated quenching of the excited intermediate, enabling up to 3000-fold enhancement of light-emission intensity. These probes found widespread applications in bioimaging, immunoassays, and real-time monitoring of cellular events both in vitro and in vivo . − Here, we report a highly sensitive chemiluminescent probe for the detection of PYRase and its use for the rapid screening of its activity across numerous Gram-positive and Gram-negative bacterial strains.…”