A Missense Mutation in the RING Finger Motif of PEX2 Protein Disturbs the Import of Peroxisome Targeting Signal 1 (PTS1)-Containing Protein but Not the PTS2-Containing Protein

Huang, Yuan; Ito, Ritsu; Miura, Satoshi; Hashimoto, Takashi; Ito, Masaki

doi:10.1006/bbrc.2000.2510

Cited by 21 publications

(18 citation statements)

References 13 publications

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“…This result also meant that although the import of both PTS1 and PTS2 requires Pex13p, the two pathways utilize different regions within Pex13p and therefore do not coincide at the stage of Pex13p in a more narrow sense. Mutations that specifically block one of the two routes have also been found previously for Pex8p and Pex2p, two peroxins that are possibly involved in later steps of the import cascade (22,25).…”

Section: Discussionsupporting

confidence: 68%

Interactions of Pex7p and Pex18p/Pex21p with the Peroxisomal Docking Machinery: Implications for the First Steps in PTS2 Protein Import

Stein

Schell-Steven

Erdmann

et al. 2002

Molecular and Cellular Biology

108

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Peroxisomal PTS2-dependent matrix protein import starts with the recognition of the PTS2 targeting signal by the import receptor Pex7p. Subsequently, the formed Pex7p/cargo complex is transported from the cytosol to the peroxisomal docking complex, consisting of Pex13p and Pex14p. In Saccharomyces cerevisiae, the latter event is thought to require the redundant Pex18p and Pex21p. Here we mapped the Pex7p interaction domain of Pex13p to its N-terminal 100 amino acids. Pex18p and Pex21p also interacted with this region, albeit only in the presence of Pex7p. Expression of an N-terminally deleted version of Pex13p in a pex13⌬ mutant failed to restore growth on fatty acids due to a specific defect in the import of PTS2-containing proteins. We further show by yeast two-hybrid analysis, coimmunoprecipitation, and in vitro binding assays that Pex7p can bind Pex13p and Pex14p in the absence of Pex18p/Pex21p. The PTS2 protein thiolase was shown to interact with Pex14p but not with Pex13p in a Pex7p-and Pex18p/Pex21p-dependent manner, suggesting that only Pex14p binds cargo-loaded PTS2 receptor. We also found that the cytosolic Pex7p/thiolase-containing complex includes Pex18p. This complex accumulated in docking mutants but was absent in cells lacking Pex18p/Pex21p, indicating that Pex18p/Pex21p are required already before the docking event.

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Section: Discussionsupporting

confidence: 68%

Interactions of Pex7p and Pex18p/Pex21p with the Peroxisomal Docking Machinery: Implications for the First Steps in PTS2 Protein Import

Stein

Schell-Steven

Erdmann

et al. 2002

Molecular and Cellular Biology

108

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“…In turn, Pex7p binds to Pex14p while Pex5p binds to Pex13p and Pex14p which are peroxisomal membrane peroxins (Huhse et al, 1998;Shimizu et al, 1999). Translocation may occur through a channel formed by zinc-binding proteins Pex2p, Pex10p, and Pex12p (Huang et al, 2000;Okumoto et al, 2000). Inside the matrix, but close to the membrane, the PTS1 and PTS2 protein cargoes dissociate from their respective receptors.…”

Section: Synthesis and Import Of Proteins In Peroxisomal Matrixmentioning

confidence: 99%

Peroxisomes and peroxisomal disorders: The main facts

Fidaleo¹

2010

Experimental and Toxicologic Pathology

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To cite this version:Marco Fidaleo. Peroxisomes and peroxisomal disorders: The main facts. Experimental and Toxicologic Pathology, Elsevier, 2010, 62 (6) This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting galley proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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“…Different models have been proposed to explain the ensuing transfer of the protein cargo across the membrane (for a review, see Eckert and Erdmann, 2003;Gould and Collins, 2002). Genetic and biochemical analyses hint at an active role for the peroxins Pex2p, Pex10p and Pex12p in the steps that follow the docking of the Pex5p/Pex7p-bound cargo to the membrane (Chang et al, 1997;Chang et al, 1999;Huang et al, 2000;Kalish et al, 1995;Okumoto et al, 1997;Okumoto et al, 1998a;Okumoto et al, 1998b;Okumoto et al, 2000;Patarca and Fletcher, 1992;Tan et al, 1995;Tsukamoto et al, 1991). Because deleting the genes of any one of these three proteins results in the mislocalization of peroxisomal matrix proteins, and at least for ∆pex10 and ∆pex12 cells it also results in the accumulation of Pex5p at the cytosolic face of the peroxisomal membrane, a participation in the transfer of the cargo across the membrane seems plausible (Dodt and Gould, 1996).…”

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confidence: 99%

Pex10p links the ubiquitin conjugating enzyme Pex4p to the protein import machinery of the peroxisome

Eckert

Johnsson

2003

Journal of Cell Science

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The protein import machinery of the peroxisome consists of many proteins,collectively called the peroxins. By applying the split-ubiquitin technique we systematically tested the pair-wise interactions between the Nub-and Cub-labeled peroxins for the first time in the living cells of the yeast Saccharomyces cerevisiae. We found that Pex10p plays a central role in the protein interaction network by connecting the ubiquitin conjugation enzyme Pex4p to the other members of the protein import machinery. A yeast strain harboring a deletion of PEX3 enabled us to estimate the influence of the peroxisomal membrane on the formation of a subset of the investigated protein-protein interactions.

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A Missense Mutation in the RING Finger Motif of PEX2 Protein Disturbs the Import of Peroxisome Targeting Signal 1 (PTS1)-Containing Protein but Not the PTS2-Containing Protein

Cited by 21 publications

References 13 publications

Interactions of Pex7p and Pex18p/Pex21p with the Peroxisomal Docking Machinery: Implications for the First Steps in PTS2 Protein Import

Interactions of Pex7p and Pex18p/Pex21p with the Peroxisomal Docking Machinery: Implications for the First Steps in PTS2 Protein Import

Peroxisomes and peroxisomal disorders: The main facts

Pex10p links the ubiquitin conjugating enzyme Pex4p to the protein import machinery of the peroxisome

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