A missense mutation in <i>ASIP</i> is associated with light point variation in donkeys

Yu, Yan; Shang, Songyang; Zhang, Xiaoying; Wang, Zhe; Dang, Wanyi; Zhang, Junpeng; Zhu, Yanxu; Dang, Ruihua; Irwin, David M.; Zhang, Shuyi

doi:10.1111/age.12940

Cited by 2 publications

(2 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Genotyping of 13 Chinese donkey breeds showed that the mutation could distinguish the solid-black (genotype C/C) from non-black (genotype T/T and C/T) phenotype, and the Dezhou donkey had the highest (0.489) C/C genotype frequency (Sun et al, 2017). Another study further confirmed that the frequency of the ASIP T-allele correlates with the size and number of light points (Yu et al, 2020). In domestic horses of the same genus, ASIP causes melanocytes to synthesize pheomelanin instead of eumelanin by blocking the effect of MC1R, which is responsible for the variation in the shade of the bay coat color (Corbin et al, 2020).…”

Section: Discussionmentioning

confidence: 86%

Genome‐wide analyses based on a novel donkey 40K liquid chip reveal the gene responsible for coat color diversity in Chinese Dezhou donkey

Liu,

Su,

Yang

et al. 2023

Animal Genetics

Self Cite

View full text Add to dashboard Cite

Dezhou donkey is one of the representative local breeds in China, which is mainly divided into two strains: Sanfen and Wutou. There are obvious differences in coat color between the two strains. The former shows light points around the eyes, around the muzzle and under the belly, while the latter is completely solid black. In this study, genome‐wide association analysis was performed for the differences in coat color traits between the Sanfen (n = 97) and Wutou (n = 108) strains using a novel donkey 40K liquid chip developed based on GenoBaits technology, to identify genomic regions and causal genes that could explain this variation. We also used FST and The cross‐population composite likelihood ratio test (XPCLR) analyses to explore selected regions related to coat color differences. We identified one significant region on chromosome 15, with the most significant SNP located within the agouti signaling protein (ASIP) gene. At the same time, both FST and XPCLR methods detected the same selected region on chromosome 15, and ASIP was the gene with the strongest signal. ASIP and melanocortin 1 receptor (MC1R) control the ratio of eumelanin to pheomelanin through their protein activity. They are deeply involved in the process of melanosome organation and melanogenesis, thus affecting mammals’ coat color variation. We used a range of genome‐wide approach to identify the genetic basis of coat color variation in Dezhou donkeys. The results provide a supplement to the color variation study in Chinese donkeys at the genome‐wide level, and preliminarily verified the reliability of the Molbreeding Donkey No. 1 40K liquid chip.

show abstract

Section: Discussionmentioning

confidence: 86%

Genome‐wide analyses based on a novel donkey 40K liquid chip reveal the gene responsible for coat color diversity in Chinese Dezhou donkey

Liu,

Su,

Yang

et al. 2023

Animal Genetics

Self Cite

View full text Add to dashboard Cite

show abstract

“…Given that one white spotted donkey was not explained by this mutation, it is possible that other variants in KIT or other pigmentation genes, such as agouti signaling protein (ASIP), in which the non-light points mutation occurs, may play a role in white spotting phenotypes in donkeys. 12 In horses close to 40 different mutations in at least seven genes contribute to white spotting; however, in all but one white spotted donkey studied to date, the same KIT mutation (c.1978+2T>A) explains white patterning, thus supporting the notion that the domesticated donkey has had fewer selection pressures and subsequently fewer distinct phenotypes compared with the horse. Donkey white spotting is postulated to be homozygous embryonic lethal, and accordingly, 35 spotted donkeys previously reported were heterozygous for KIT (c.1978+2T>A).…”

Section: Supporting Informationmentioning

confidence: 86%