A missense mutant of the PPAR-γgene associated with carcass and meat quality traits in Chinese cattle breeds

Abstract

ABSTRACT. The peroxisome proliferator-activated receptor gamma (PPAR-γ) is a key molecule in adipocyte differentitation; it transactivates multiple target genes in lipid metabolic pathways. Using PCR-SSCP and DNA sequencing, we evaluated a potential association of an SNP (72472 G﹥T in exon7) of the bovine PPAR-γ gene with carcass and meat quality traits in 660 individuals from five Chinese indigenous cattle breeds, Qinchuan (QC), Luxi (LX), Nanyang (NY), Jiaxian (JX), and Xianan (XN). This 72472 G﹥T mutation identified a missense mutation, Q448H. Two alleles were named C and D. Allele frequencies of PPAR-γ-C/D in the five breeds were 0.78/0.22, 0.90/0.10, 0.74/0.26, 0.71/0.29, and 0.83/0.17 for QC, NY, JX, LX, and XN, respectively. Except for the XN breed, all breeds were in Hardy-Weinberg equilibrium at this locus. The polymorphism information content was low for NY and XN (0.16 and 0.24, respectively), while it was moderately high for QC, JX, and LX (0.28, 0.31 and 0.33, respectively). Correlation analysis showed significant association of this missense mutation with carcass length, backfat thickness and water holding capacity in the QC breed. Animals with the genotype CD had significantly greater carcass length than those with genotypes CC and DD, while animals with genotype CC had significantly greater backfat thickness than those with genotypes CD and DD. Animals with genotype CC had lower water holding capacity than those with the genotypes CD and DD. In conclusion, this locus is a candidate for a major quantitative trait locus affecting production traits and could be used for beef breeding selection.

Comparative analysis on genome-wide DNA methylation in longissimus dorsi muscle between Small Tailed Han and Dorper×Small Tailed Han crossbred sheep

“…Similar to RYR1 , PPARG2 is another gene with exon hypo-methylation. Polymorphisms of PPARG2 gene were reported to be associated with meat quality and production in cattle [ 15 ]. PPARG2 encodes PPAR-gamma protein which is a regulator of adipocyte differentiation.…”

Genome-wide identification and analysis of long noncoding RNAs in longissimus muscle tissue from Kazakh cattle and Xinjiang brown cattle

“…For customers, the tenderness, flavor and juiciness of beef are important factors in the judgment of beef quality [2,3]. Improving beef quality is the top priority of the beef industry because beef quality is the primary factor in determining beef consumption.…”

“…This success played a meaningful role in further improving the quality of beef in Xinjiang. The quality of beef depends on the marbling, texture, fat color, meat color and overall maturity of the meat[2].The amount of fat deposition is positively correlated with the flavor and palatability of beef[22]. The deposition of intramuscular adipose tissue affects meat quality and animal productivity.Irish consumers can judge the quality of beef with great precision[23].…”

Differential expression of mRNA-miRNAs related to intramuscular fat content in the longissimus dorsi in Xinjiang brown cattle

“…Meat quality is determined on the basis of marbling, texture, fat color, meat color and overall maturity [ 17 ]. Fat deposition is positively related to beef flavor and palatability.…”