A Miso (Japanese Soybean Paste) Diet Conferred Greater Protection against Hypertension than a Sodium Chloride Diet in Dahl Salt-Sensitive Rats

Watanabe, Haruo; Kashimoto, Naoki; Kajimura, Junko; Kamiya, Kenji

doi:10.1291/hypres.29.731

Cited by 39 publications

(34 citation statements)

References 24 publications

(27 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To date, clinical studies provided evidence that small peptides have an ability to elicit antihypertensive effect in mild hypertensive subjects (Hata and others 1996; Kawasaki and others 2000). An interesting finding was also reported that 10% miso‐diet containing 2.3% NaCl significantly suppressed a promoting BP rise in salt‐sensitive Dahl rats compared with control diet containing 2.3% NaCl (Watanabe and others 2006). Although the mechanism remains unclear, it seems likely that some peptides in miso paste may be responsible for the effect via, for example, angiotensin I‐converting enzyme (ACE, EC 3.4.15.1) inhibition (Hata and others 1996), L‐type Ca 2+ channel blocker (Matsui and others 2005), or heme oxygenase‐1 activator (Erdmann and others 2006).…”

Section: Introductionmentioning

confidence: 74%

Antihypertensive Effect of Salt‐Free Soy Sauce, a New Fermented Seasoning, in Spontaneously Hypertensive Rats

Matsui¹,

Zhu²,

Shiraishi³

et al. 2010

Journal of Food Science

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 74%

Antihypertensive Effect of Salt‐Free Soy Sauce, a New Fermented Seasoning, in Spontaneously Hypertensive Rats

Matsui¹,

Zhu²,

Shiraishi³

et al. 2010

Journal of Food Science

View full text Add to dashboard Cite

show abstract

“…As described before, unlike the cases of salt alone, the salt in miso reduced stomach cancer 72 , and miso did not cause an increase in gastric tumors. So the effects of salt in miso on blood pressure were studied 88 .…”

Section: Protection Against Hypertensionmentioning

confidence: 99%

“… Systolic blood pressure in female rats 88 . *Significantly different from same strain control (p<0.05).…”

Section: Protection Against Hypertensionmentioning

confidence: 99%

Beneficial Biological Effects of Miso with Reference to Radiation Injury, Cancer and Hypertension

Watanabe

2013

J Toxicol Pathol

Self Cite

View full text Add to dashboard Cite

This review describes effects of miso with reference to prevention of radiation injury, cancer and hypertension with a twin focus on epidemiological and experimental evidence. Miso with a longer fermentation time increased crypt survival against radiation injury in mice. When evaluating different types of miso provided by different areas in Japan, miso fermented for a longer period increased the number of surviving crypts, and 180 days of fermentation was the most significant. Dietary administration of 180-day fermented miso inhibits the development of azoxymethane (AOM)-induced aberrant crypt foci (ACF) and rat colon cancers in F344 rats. Miso was also effective in suppression of lung tumors, breast tumors in rats and liver tumors in mice. The incidence of gastric tumors of groups of rats given NaCl was higher than those of the groups given miso fermented for longer periods. Moreover, the systolic blood pressure of the Dahl male rat on 2.3% NaCl was significantly increased but that of the SD rat was not. However, the blood pressures of the rats on a diet of miso or commercial control diet (MF) did not increase. Even though miso contains 2.3% NaCl, their blood pressures were as stable as those of rats fed commercial diet containing 0.3% salt. So we considered that sodium in miso might behave differently compared with NaCl alone. These biological effects might be caused by longer fermentation periods.

show abstract

“…One of the inactivation pathways of CAs is the enzymatic metabolism by catechol-O-methyltransferase (COMT; EC2.1.1.6), which methylates their catechol moieties using S-adenosyl-L-methionine (SAMe) as a methyl donor.1) There are two COMT isoforms: in the cytoplasm as soluble COMT (S-COMT) and in association with membranes as membrane-bound COMT (MB-COMT).We have previously reported a rapid assay method for COMT activities using high-performance liquid chromatography (HPLC)-fluorescence detection.2) In this study, we applied the method to brain tissues, such as cerebral cortex, cerebellum, hypophysis and hypothalamus.In previous report using Sprague-Dawley (SD) rats, the cerebral cortex and the cerebellum were seemed to be important parts in brain for the metabolism of NE.3) Furthermore, the experiment with spontaneously hypertensive rats (SHR) suggested an association between MB-COMT in the cerebral cortex and blood pressure.4) Hence, in order to clarify the role of COMT in rat brain in relation to salt-sensitive hypertension, COMT activities in Dahl salt-sensitive (DS) rats, 5,6) which are model rats of salt-sensitive hypertension, were also examined. …”

mentioning

confidence: 99%

“…4) Hence, in order to clarify the role of COMT in rat brain in relation to salt-sensitive hypertension, COMT activities in Dahl salt-sensitive (DS) rats, 5,6) which are model rats of salt-sensitive hypertension, were also examined.…”

mentioning

confidence: 99%

Measurement of Catechol-O-methyltransferase Activity in the Brain of Dahl Salt-Sensitive Rats

Hirano

Tsunoda

Shimosawa

et al. 2007

Biological & Pharmaceutical Bulletin

View full text Add to dashboard Cite

Catecholamines (CAs; norepinephrine (NE), dopamine and epinephrine) play important roles not only in the peripheral nervous system but in the central one. One of the inactivation pathways of CAs is the enzymatic metabolism by catechol-O-methyltransferase (COMT; EC2.1.1.6), which methylates their catechol moieties using S-adenosyl-L-methionine (SAMe) as a methyl donor.1) There are two COMT isoforms: in the cytoplasm as soluble COMT (S-COMT) and in association with membranes as membrane-bound COMT (MB-COMT).We have previously reported a rapid assay method for COMT activities using high-performance liquid chromatography (HPLC)-fluorescence detection.2) In this study, we applied the method to brain tissues, such as cerebral cortex, cerebellum, hypophysis and hypothalamus.In previous report using Sprague-Dawley (SD) rats, the cerebral cortex and the cerebellum were seemed to be important parts in brain for the metabolism of NE.3) Furthermore, the experiment with spontaneously hypertensive rats (SHR) suggested an association between MB-COMT in the cerebral cortex and blood pressure.4) Hence, in order to clarify the role of COMT in rat brain in relation to salt-sensitive hypertension, COMT activities in Dahl salt-sensitive (DS) rats, 5,6) which are model rats of salt-sensitive hypertension, were also examined. MATERIALS AND METHODSReagent NE, normetanephrine (NMN), SAMe chloride salt and 4-hydroxy-3-methoxybenzylamine (HMBA) were purchased from Sigma (St. Louis, MO, U.S.A.). Ethylenediamine was from Sigma-Aldrich (Milwaukee, WI, U.S.A.). Imidazole and 1,4-dithiothreitol were obtained from Merck (Darmstadt, Germany). Acetonitrile and ethanol, both of HPLC grade, were purchased from Kanto Chemical Co. (Tokyo, Japan). All other reagents were of analytical grade.Animals Male DS and Dahl salt-resistant (DR) rats (6-7 weeks old) were purchased from the Shizuoka Laboratory Animal Center (Shizuoka, Japan). The rats were randomly assigned to the normal-salt (NS, 0.3% NaCl) or high-salt (HS, 8% NaCl) group. The animals were housed under controlled environment (22-24°C and a 12-h light-dark cycle) with free access to tap water and diet. Systolic blood pressure was measured by the tail-cuff method every 2 weeks. All animals received human care in compliance with the National Institute of Health guideline.Preparation of Brain COMT Samples In anesthetized rats with pentobarbital, blood was removed from inferior vena cava, and cerebral cortex, cerebellum, hypophysis and hypothalamus were immediately removed and chilled on ice. All further procedures were the same with the previous reports.2,3) Each tissue was weighted and then homogenized. The homogenates were centrifuged at 100000ϫg for 30 min and the supernatants were stored for the determination of S-COMT. The pellet was washed and re-centrifuged at 100000ϫg for 30 min. Then, the pellet was suspended in buffer for the MB-COMT.COMT Assay Forty microliters of the S-COMT sample was incubated with 50 mM sodium phosphate buffer (pH 7.8) containing 1.5 mM NE, 2 mM MgCl 2 and 200 mM SAMe...

show abstract

A Miso (Japanese Soybean Paste) Diet Conferred Greater Protection against Hypertension than a Sodium Chloride Diet in Dahl Salt-Sensitive Rats

Cited by 39 publications

References 24 publications

Antihypertensive Effect of Salt‐Free Soy Sauce, a New Fermented Seasoning, in Spontaneously Hypertensive Rats

Antihypertensive Effect of Salt‐Free Soy Sauce, a New Fermented Seasoning, in Spontaneously Hypertensive Rats

Beneficial Biological Effects of Miso with Reference to Radiation Injury, Cancer and Hypertension

Measurement of Catechol-O-methyltransferase Activity in the Brain of Dahl Salt-Sensitive Rats

Contact Info

Product

Resources

About