A miR-511-binding site SNP in the 3′UTR of IGF-1 gene is associated with proliferation and apoptosis of PK-15 cells

Wei, Wen‐Cheng J.; Wang, Gang; Cheng, Yi; Yang, Rui; Song, Jié; Huang, Shan; Li, Haoyang; Geng, Hao; Yu, Hao; Liu, Songcai; Hao, Linlin

doi:10.1007/s11626-019-00329-4

Cited by 7 publications

(4 citation statements)

References 51 publications

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“…Meanwhile the PI3K/AKT/FoxO1 pathway is implicated in the mechanism of action of the flavone baicalin in alleviating depression-like behaviors by suppressing the Toll-like receptor TLR4 (Guo et al, 2019). Moreover, miR-511 significantly accelerated procine kidney (PK-15) cell apoptosis by reducing the extent of AKT phosphorylation (Wei et al, 2019). Herein, from the aforementioned findings, we might hypothesize that GP could affect the progression of CUMS-induced depression by mediating Six3os1, acting in association with regulation of the miR-511-3p/Fezf1/AKT axis.…”

Section: Introductionmentioning

confidence: 99%

Geniposide Alleviates Oxidative Stress of Mice With Depression-Like Behaviors by Upregulating Six3os1

Zou

Sugimoto

Zhang

et al. 2020

Front. Cell Dev. Biol.

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Depression is a major cause of disease burden and severely impairs well-being of patients around the globe. Geniposide (GP) has been revealed to play a significant role in depression treatment. Of note, RNA sequencing of this study identified highly expressed long non-coding RNA Six3os1 in response to GP treatment. Thus, we aim to explore how GP affected chronic unpredictable mild stress (CUMS)-induced depressionlike behaviors in mice in vivo and in vitro and the downstream molecular mechanism related to Six3os1. The relationship of Six3os1, miR-511-3p and Fezf1 was evaluated by dual-luciferase reporter gene assay, RIP assay, and RNA pulling down assay. Ectopic expression and knockdown experiments were developed in CUMS-induced mice and neurons with or without GP treatment. In vitro experiments and behavioral tests were conducted to examine alteration of CUMS-triggered oxidative stress following different interferences. The experimental data validated that GP treatment resulted in high expression of Six3os1 and Fezf1 and poor expression of miR-511-3p in CUMS-induced neurons. Six3os1 activated the AKT signaling pathway by upregulating miR-511-3ptargeted Fezf1. Either GP treatment or overexpression of Six3os1 or Fezf1 alleviated depression-like behaviors of CUMS-induced mice. GP treatment, miR-511-3p inhibition or overexpression of Six3os1 or Fezf1 not only reduced oxidative stress in CUMSinduced mice and neurons, but also reduced CUMS-induced neuronal apoptosis. Collectively, GP treatment-mediated Six3os1 upregulation ameliorated oxidative stress of mice with depression-like behaviors via the miR-511-3p/Fezf1/AKT axis.

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Section: Introductionmentioning

confidence: 99%

Geniposide Alleviates Oxidative Stress of Mice With Depression-Like Behaviors by Upregulating Six3os1

Zou

Sugimoto

Zhang

et al. 2020

Front. Cell Dev. Biol.

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“…Researches revealed different targets of miR-511, such as AKT1, Insulin-like growth factor-1, Hepatomaderived growth factor, TLR4, NO synthase 1 and TRIM24 [5,18,[22][23][24][25]. In present study, the interaction has been predicted in targetscan (http://www.…”

Section: Discussionmentioning

confidence: 60%

MiR-511 mimic transfection inhibits the proliferation, invasion of osteosarcoma cells and reduces metastatic osteosarcoma tumor burden in nude mice via targeting MAPK1

Wu¹,

Zhang²,

Chen³

2019

CBM

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Osteosarcoma, a highly aggressive cancer, can rapidly metastasize to distant organs such as lung, liver, brain. Despite much progress in the therapeutic regime has been made, the prognosis of osteosarcoma remains poor. In present study, microRNA-511 (miR-511) is lowly expressed in osteosarcoma cells, including MG63, U-2 OS, Saos-2 cells, while mitogen activated protein kinase1 (MAPK1) is highly expressed in osteosarcoma cells. Interestingly, MAPK1 might be a target of miR-511. We found that overexpression of miR-511 by miR-511 mimic transfection may result to low expression of MAPK1. Further study showed that miR-511 mimic inhibits the development of osteosarcoma MG63 cell, including proliferation and invasion. Moreover, miR-511 mimic transfection reduces metastatic osteosarcoma tumor burden in nude mice. These activities are mediated by targeting MAPK1. Our study provides a new sight for the molecular pathogenesis of osteosarcoma.

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“…After overexpressing the rat IGF1 gene in the liver of IGF1 -null mice, the transgene was found to maintain normal post-natal growth by elevating circulating IGF1 levels ( 52 ). In vitro IGF1 has been shown to be associated with cell proliferation and apoptosis in various cells, such as mesenchymal progenitor cells, intimal smooth muscle cells, and porcine kidney cells ( 53 , 54 ). In this study, we found that the overexpression of any of the four IGF1 splice variants significantly increased cell proliferation and inhibited apoptosis ( P < 0.01).…”

Section: Discussionmentioning

confidence: 99%

Molecular cloning, expression, and functional features of IGF1 splice variants in sheep

Song

Zhang

Zhao

et al. 2021

Endocrine Connections

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Insulin-like growth factor 1 (IGF1),also known as somatomedin C, is essential for the regulation of animal growth and development. In many species, the IGF1 gene can be alternatively spliced into multiple transcripts, encoding different pre-pro-IGF1 proteins. However, the exact alternative splicing patterns of IGF1 and the sequence information of different splice variants in sheep are still unclear. In this study, four splice variants (class 1-Ea, class 1-Eb, class 2-Ea, and class 2-Eb) were obtained, but no IGF1 Ec, similar to that found in other species, was discovered. Bioinformatics analysis showed that the four splice variants shared the same mature peptide (70 amino acids) and possessed distinct signal peptides and E peptides. Tissue expression analysis indicated that the four splice variants were broadly expressed in all tested tissues and were most abundantly expressed in the liver. In most tissues and stages, the expression of class 1-Ea was highest, and the expression of other splice variants was low. Overall, levels of the four IGF1 splice variants at the fetal and lamb stages were higher than those at the adult stage. Overexpression of the four splice variants significantly increased fibroblast proliferation and inhibited apoptosis (P < 0.05). In contrast, silencing IGF1 Ea or IGF1 Eb with siRNA significantly inhibited proliferation and promoted apoptosis (P < 0.05). Among the four splice variants, class 1-Ea had a more evident effect on cell proliferation and apoptosis. In summary, the four ovine IGF1 splice variants have different structures and expression patterns and might have different biological functions.

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A miR-511-binding site SNP in the 3′UTR of IGF-1 gene is associated with proliferation and apoptosis of PK-15 cells

Cited by 7 publications

References 51 publications

Geniposide Alleviates Oxidative Stress of Mice With Depression-Like Behaviors by Upregulating Six3os1

Geniposide Alleviates Oxidative Stress of Mice With Depression-Like Behaviors by Upregulating Six3os1

MiR-511 mimic transfection inhibits the proliferation, invasion of osteosarcoma cells and reduces metastatic osteosarcoma tumor burden in nude mice via targeting MAPK1

Molecular cloning, expression, and functional features of IGF1 splice variants in sheep

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