“…With the advancement of polymer-based microfabrication technology [5], contemporary microfluidics is no longer restricted to silicon-based devices and can be biocompatible. Various microfluidic devices, such as micropump [6,7], microvalve [8,9], micromixer [10,11] and microfluidic flow sensors [12][13][14] were developed with more robust functionalities. Integrated microfluidic systems, which are composed of multiple microfluidic components, have been typically applied to manipulate, regenerate and sense chemicals and bio-fluids with volumes ranging from micro-liters down to pico-liters [15][16][17][18][19] for delicate and sensitive biochemical applications, e.g., single-cell [20], subcellular [21] and single-molecule [22] analyses.…”