A minor E-selectin ligand, CD65, is critical for extravascular infiltration of acute myeloid leukemia cells

Noguchi, Masaaki; Sato, Naotake; Sugimori, Hiroki; Mori, Kiyoshi; Oshimi, Kazuo

doi:10.1016/s0145-2126(01)00036-4

Cited by 20 publications

(21 citation statements)

References 19 publications

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“…Protein detection wasperformed by the ECL Plus western blotting detection reagent. Panel A: molecular weight ladder; Panel B: proteins of SVF cells from subcutaneous tissue; Panel C: proteins of mature adipocytes from subcutaneous tissue; Panel D: proteins of SVF cells from omental tissue; Panel E: proteins of mature adipocytes from omental tissue tins (Noguchi et al 2001), on mature adipocytes. This result consolidates the hypothesis that adipocytes are implicated in leucocyte recruitment via CD34 and CD65.…”

Section: Discussionmentioning

confidence: 99%

Surface protein expression between human adipose tissue-derived stromal cells and mature adipocytes

Festy¹,

Hoareau²,

Bès-Houtmann³

et al. 2005

Histochem Cell Biol

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Adipose tissue contains a stroma that can be easily isolated. Thus, human adipose tissue presents an source of multipotent stromal cells. In order to determine the implication of hematopoietic markers in adipocyte biology, we have defined part of the phenotype of the human adipose tissue-derived stromal cells, and compared this to fully differentiated adipocytes. Flow cytometry demonstrates that the protein expression phenotype of both cell types are similar and includes the expression of CD10, CD13, CD34, CD36, CD55, CD59 and CD65. No significant difference between subcutaneous and omental adipose tissue could be demonstrated concerning the expression of these markers. However, the expression of CD34, CD36 and CD65 is cell-dependent. While the expression of CD36 and CD65 doubled between stromal cells and mature adipocytes, the expression of CD34 decreased, despite this protein being present on the mature adipocyte. As CD34 is described as a stem cell marker and it being unlikely to be expressed on differentiated cells, this result was confirmed by immunostaining and western blot. The clear function of this protein on the adipocyte membrane remains to be determined. The characterization of new proteins on mature adipocytes could have broad implications for the comprehension of the biology of this tissue.

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Section: Discussionmentioning

confidence: 99%

Surface protein expression between human adipose tissue-derived stromal cells and mature adipocytes

Festy¹,

Hoareau²,

Bès-Houtmann³

et al. 2005

Histochem Cell Biol

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“…125 In accordance, high infiltration of leukemic blasts in patients with AML strongly correlated with increased expression of both ␣ L ␤ 2 -and ␣ M ␤ 2 -integrins. 121 AML cell adhesion to bone marrow fibroblast monolayers seems to require both ␤ 1 -and ␤ 2 -integrins, as antibodies against them inhibited the binding. 126 Interaction between leukemic cells and bone marrow stroma cells has been shown to increase leukemic cell survival and chemotherapy-induced leukemia cell resistance.…”

Section: Role Of Integrins and Gelatinases In Acute Leukemiasmentioning

confidence: 99%

“…120 Several reports have demonstrated the involvement of both MMP-2/-9 gelatinases and ␤ 2 -integrins in the growth and progression of myeloid and lymphoid neoplasms. 121,122 Selective MMP-9 expression is induced as a result of ␣ M ␤ 2 -integrin ligation in PMNs 123 and ␣ L ␤ 2 -integrin ligation in T-lymphoma cells. 124 Also, studies from ␣ M -and ␣ L -integrin knock-out mice confirm the importance of ␤ 2 -integrins in mediating leukocyte adhesion and migration.…”

Section: Role Of Integrins and Gelatinases In Acute Leukemiasmentioning

confidence: 99%

Cell-surface association between matrix metalloproteinases and integrins: role of the complexes in leukocyte migration and cancer progression

Stefanidakis

Koivunen

2006

Blood

145

105

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Leukocyte motility is known to be dependent on both ␤2-integrins and matrix metalloproteinases MMP-2/-9 or gelatinases, which mediate leukocyte adhesion and the proteolysis needed for invasion, respectively. Gelatinases not only play an important role in cell migration, tissue remodeling, and angiogenesis during development, but are also involved in the progression and invasiveness of many cancers, including leukemias. The concept that MMPs associate with integrins, as well as their importance in some physiologic and pathologic conditions, has been advanced previously but has not been examined on leukocytes. This review will examine mainly the function of the MMP-integrin complexes in normal leukocyte migration and the effect of integrin and broad-spectrum MMP inhibitors in tumor

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“…CD15 and CD65 are two carbohydrate motifs, respectively the 3-fucosyl-N-acetyl-lactosamine and the ceramide dodecasaccharide [37], that can be carried by various proteins. CD15 is also known as the Lexis × antigen.…”

Section: Myeloid-lineage Differentiation Antigensmentioning

confidence: 99%

Immunophenotyping of acute leukaemias

Béné¹

2005

Immunology Letters

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A minor E-selectin ligand, CD65, is critical for extravascular infiltration of acute myeloid leukemia cells

Cited by 20 publications

References 19 publications

Surface protein expression between human adipose tissue-derived stromal cells and mature adipocytes

Surface protein expression between human adipose tissue-derived stromal cells and mature adipocytes

Cell-surface association between matrix metalloproteinases and integrins: role of the complexes in leukocyte migration and cancer progression

Immunophenotyping of acute leukaemias

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