A Minimally Invasive Lesion Technique for Muscles Intrinsic to the Odontophore of <em>Aplysia californica</em>

Abstract

Aplysia californica is a model system for studying the neural control of learning and behavior. This animal has a semi-open circulatory system, making it possible to access many of its internal structures without causing any significant damage. Many manipulations can be easily performed both in vivo and in vitro, making it a highly tractable model for the analysis of behavior and neural circuitry. To better understand the functions of muscles within the feeding grasper, we have developed a technique for lesioning them without opening the main body cavity of the animal or damaging the outer layers of the feeding organ (i.e., the buccal mass). In this technique, the grasper is partially everted, allowing direct access to the musculature. This procedure allows animals to recover quickly and reliably. This has made it possible to lesion the I7 muscles and subradular fibers, allowing us to show that both muscles significantly contribute to the opening in vivo.

Reciclagem e Aplicações da Borracha Sintética de Chinelos Descartados no Design de Produtos

“…O aglutinante bi componente da marca KEHL possui em sua composição, componentes renováveis como óleos de origem vegetal que não causam impactos negativos no meio ambiente (KEHL, 2016). A empresa Kehl atuou como parceira fornecendo os aglutinantes para o projeto.…”