A minimally invasive lens-free computational microendoscope

Shin, Jaewook; Trần, Dũng; Stroud, Jasper R.; Chin, Sang; Tran, Trac D.; Foster, Mark A.

doi:10.1126/sciadv.aaw5595

Cited by 65 publications

(33 citation statements)

References 27 publications

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“…In addition, these mask-only systems have no magnifying optics and thus are limited to a low effective numerical aperture (NA), resulting in poor lateral and axial resolutions. Other recent work combines coding elements with multi-fiber endoscopes to achieve single-shot non-fluorescence 3D, with relatively low resolution 18 . Recently, the miniature light-field microscope (MiniLFM) 19 demonstrated an integrated 3D fluorescence system with computationally efficient temporal video processing for neural activity tracking 20 .…”

Section: Introductionmentioning

confidence: 99%

Miniscope3D: optimized single-shot miniature 3D fluorescence microscopy

et al. 2020

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Miniature fluorescence microscopes are a standard tool in systems biology. However, widefield miniature microscopes capture only 2D information, and modifications that enable 3D capabilities increase the size and weight and have poor resolution outside a narrow depth range. Here, we achieve the 3D capability by replacing the tube lens of a conventional 2D Miniscope with an optimized multifocal phase mask at the objective’s aperture stop. Placing the phase mask at the aperture stop significantly reduces the size of the device, and varying the focal lengths enables a uniform resolution across a wide depth range. The phase mask encodes the 3D fluorescence intensity into a single 2D measurement, and the 3D volume is recovered by solving a sparsity-constrained inverse problem. We provide methods for designing and fabricating the phase mask and an efficient forward model that accounts for the field-varying aberrations in miniature objectives. We demonstrate a prototype that is 17 mm tall and weighs 2.5 grams, achieving 2.76 μm lateral, and 15 μm axial resolution across most of the 900 × 700 × 390 μm3 volume at 40 volumes per second. The performance is validated experimentally on resolution targets, dynamic biological samples, and mouse brain tissue. Compared with existing miniature single-shot volume-capture implementations, our system is smaller and lighter and achieves a more than 2× better lateral and axial resolution throughout a 10× larger usable depth range. Our microscope design provides single-shot 3D imaging for applications where a compact platform matters, such as volumetric neural imaging in freely moving animals and 3D motion studies of dynamic samples in incubators and lab-on-a-chip devices.

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Section: Introductionmentioning

confidence: 99%

Miniscope3D: optimized single-shot miniature 3D fluorescence microscopy

et al. 2020

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“…A significant transformation has occurred in the field of minimally invasive cardiac surgery, from continuous refinements of the results of minimal access methods to the application of transcatheter solutions for heart disease [3,4] . With the development of minimally invasive surgery and optical imaging techniques, endoscopic imaging is widely used in the interior of the human body, enabling disease diagnosis and surgical image guidance [5][6][7][8][9] . For example, nonlinear optical endoscopy was developed for three-dimensional (3D) optical imaging [10] , and fluorescent endoscopy can provide realtime cellular imaging [11,12] .…”

Section: Introductionmentioning

confidence: 99%

Design of a real-time fiber-optic infrared imaging system with wide-angle and large depth of field

Peng

2022

中国光学快报

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A key limitation in the observation of instruments used in operations and heart sutures during a procedure is the scattering and absorption during optical imaging in the presence of blood. Therefore, we propose a novel real-time fiber-optic infrared imaging system simultaneously capturing a flexible wide field of view (FOV) and large depth of field infrared image in real time. The assessment criteria for imaging quality of the objective and coupling lens have been optimized and evaluated. Furthermore, the feasibility of manufacturing and assembly has been demonstrated with tolerance sensitivity and the Monte Carlo analysis. The simulated results show that the optical system can achieve a large working distance of 8 to 25 mm, a wide FOV of 120°, and the relative illuminance is over 0.98 in the overall FOV. To achieve high imaging quality in the proposed system, the modulation transfer function is over 0.661 at 16.7 lp/mm for a 320 × 256 short wavelength infrared camera sensor with a pixel size of 30 μm.

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“…An emerging set of approaches for lensless endoscopes that go beyond many of the conventional limitations are based on the measurement of the fiber transmission-matrix (TM), with or without an additional distal mask or diffuser. This allows compensation of the complex coherent or incoherent transfer-function of these multi-mode systems, either digitally [10,11,12,13,14,15,16] or via wavefront-shaping [17,18,19,20,21,22,23,24]. However, solutions that are based on a single TM measurement without the addition of a distal element is limited to non-flexible fibers, since fiber bending strongly distorts the TM.…”

Section: Main Text Introductionmentioning

confidence: 99%

“…The TM may be estimated using a large number of measurements and complex computation, but do not allow video-rate imaging or imaging through dynamically bent fibers [25,26,27]. Alternative, speckle based incoherent imaging approaches [11,12,13,14], suffer from very low contrast of the acquired images. MCFs with bend-insensitive intercore phase relations have been fabricated [28].…”

Section: Main Text Introductionmentioning

confidence: 99%

Real-time holographic lensless micro-endoscopy through flexible fibers via Fiber Bundle Distal Holography (FiDHo)

Badt¹,

Katz²

2021

Preprint

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Fiber-based micro-endoscopes are a critically important tool for minimally-invasive deep-tissue imaging. However, the state-of-the-art micro-endoscopes cannot perform three-dimensional imaging through dynamically-bent fibers without the use of bulky optical elements such as lenses and scanners at the distal end, increasing the footprint and tissue-damage. Great efforts have been invested in developing approaches that avoid distal bulky optical elements. However, the fundamental barrier of dynamic optical wavefront-distortions in propagation through flexible fibers, limits current approaches to nearly-static or non-flexible fibers. Here, we present an approach that allows holographic 3D bend-insensitive, coherence-gated, micro-endoscopic imaging, using commercially available multi-core fibers (MCFs). We achieve this by adding a miniature partially-reflecting mirror to the distal fiber-tip, allowing to perform low-coherence full-field phase-shifting holography. We demonstrate widefield diffraction-limited reflection imaging of amplitude and phase targets through dynamically bent fibers at video-rates. Our approach holds potential for label-free investigations of dynamic samples

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A minimally invasive lens-free computational microendoscope

Cited by 65 publications

References 27 publications

Miniscope3D: optimized single-shot miniature 3D fluorescence microscopy

Miniscope3D: optimized single-shot miniature 3D fluorescence microscopy

Design of a real-time fiber-optic infrared imaging system with wide-angle and large depth of field

Real-time holographic lensless micro-endoscopy through flexible fibers via Fiber Bundle Distal Holography (FiDHo)

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