A Minimally Invasive Annulus Fibrosus Needle Puncture Model of Intervertebral Disc Degeneration in Rats

Zhu, Pengfei; Kong, Fanchen; Wu, Xiexing; Dong, Zhongchen; Du, Jianjun; Mao, Yubo; Zhou, Hong; Liu, Yijie; Mao, Haiqin; Gu, Ye; Yang, Huilin; Geng, Dechun

doi:10.1016/j.wneu.2022.09.062

Cited by 3 publications

(1 citation statement)

References 29 publications

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“…Furthermore, we were not able to obtain many samples because we used strict criteria to ensure that the patient only had LDH. Finally, it is worth mentioning that because intervertebral disc degeneration is a long-term and complex process, the current animal models of intervertebral disc degeneration are mostly caused by mechanical damage ( 40 ), and there is no model that is very consistent with the upright characteristics of the human spine, our research has not yet involved animal-level verification. However, we believe that exciting models will be developed in the near future.…”

Section: Discussionmentioning

confidence: 99%

Increased levels of circulating granulocytic myeloid‑derived suppressor cells in lumbar disc herniation

Zhou

Liu

et al. 2023

Exp Ther Med

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Myeloid-derived suppressor cells (MDSCs) expand when the body undergoes inflammatory diseases and chronic diseases. However, its role in intervertebral disc degeneration remains unclear. The present study aimed to characterize specific subsets of MDSCs as potential indicators of disease progression in patients with lumbar disc herniation (LDH). The Gene Expression Omnibus (GEO) database was used to analyze the changes in granulocyte MDSCs (G-MDSCs). Peripheral blood samples were collected from 40 patients with LDH and 15 healthy controls, and flow cytometry was used to characterize different subsets of MDSCs. All subjects underwent lumbar spine magnetic resonance imaging. Then, t-distributed stochastic neighborhood embedding and FlowSOM were used to analyze the data obtained by CytoFlex. The correlation between circulating MDSCs and the clinicopathological stage of LDH was then further analyzed. The GEO database predicted that G-MDSCs were highly expressed in patients with LDH. The frequency of circulating G-MDSCs increased with Pfirrmann stage III and IV, while the percentage of mononuclear MDSCs (M-MDSCs) only increased. Patient age and sex did not correlate with the frequency of circulating G-MDSCs and M-MDSCs. The results of the computer algorithm analysis were consistent with those of our manual gating. The present study showed that the occurrence of LDH led to changes in the MDSC subpopulation in the circulating peripheral blood of patients, and the frequency of circulating G-MDSCs in patients with clinical stage III and IV LDH increased with the degree of degeneration. The determination of G-MDSCs can be used as an auxiliary examination item for LDH.

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Section: Discussionmentioning

confidence: 99%

Increased levels of circulating granulocytic myeloid‑derived suppressor cells in lumbar disc herniation

Zhou

Liu

et al. 2023

Exp Ther Med

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Establishment of intervertebral disc degeneration models; A review of the currently used models

Elmounedi,

Keskes

2024

Journal of Orthopaedics

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Effectivity of puncture method for intervertebral disc degeneration animal models: review article

Romaniyanto,

Mahyudin,

Utomo

et al. 2023

Annals of Medicine &Amp; Surgery

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Intervertebral disk degeneration (IDD) is a chronic condition brought on by various factors and mechanisms that have been linked to many deaths and illnesses. The causes of IDD involve multiple processes, including genetics, stress, cellular aging, and changes in nutrition due to the limited blood supply. Animal models play a crucial role in biomedical research and the selection of these models is based on many considerations, including the need for similarities in structure and function with humans. This is important because the etiology and pathogenesis of IDD are complex. Finding the right animal model is not an easy task. In addition to having similarities to humans, these models should also be reliable, reproducible, cost-effective, and easy to maintain. One common method of inducing IDD in animal models is needle puncture. This method is less invasive and time-consuming compared to other methods and allows for precise control over the extent and location of the injury.

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A Minimally Invasive Annulus Fibrosus Needle Puncture Model of Intervertebral Disc Degeneration in Rats

Cited by 3 publications

References 29 publications

Increased levels of circulating granulocytic myeloid‑derived suppressor cells in lumbar disc herniation

Increased levels of circulating granulocytic myeloid‑derived suppressor cells in lumbar disc herniation

Establishment of intervertebral disc degeneration models; A review of the currently used models

Effectivity of puncture method for intervertebral disc degeneration animal models: review article

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