A minichromosome carrying a pigmentation gene and brook trout DNA sequences in transgenic rainbow trout

Peek, Andrew S.; Wheeler, Paul; Ostberg, Carl O.; Thorgaard, Gary H.

doi:10.1139/g97-778

Cited by 12 publications

(6 citation statements)

References 39 publications

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“…The microchromosomes are unstable components of the Amazon Molly genome and only some of them are transmitted to the offspring (Schartl et al, 1995). In chromosome-mediated transgenic rainbow trout, fragments of brook trout (Salvelinus fontinalis) chromosomes show high, mosaic stability in adults; moreover, they can be transcriptionally active and, as minichromosomes, transmitted to the next generations (Disney et al, 1987(Disney et al, , 1988Peek et al, 1997). Mechanisms of their stability are not elucidated yet (Disney et al, 1988).…”

Section: Discussionmentioning

confidence: 99%

The stability of telomereless chromosome fragments in adult androgenetic rainbow trout

Ocalewicz

Babiak

Dobosz

et al. 2004

Journal of Experimental Biology

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SUMMARY The study provides new data on the stability of gamma radiation-induced chromosome fragments of a putative maternal nuclear genome in an androgenetic vertebrate, rainbow trout (Oncorhynchus mykiss Walbaum). The fragments were found in five of 16 examined individuals and they were mostly centromeric parts of metacentric or subtelocentric chromosomes. Chromosome fragments were identical in all cells of a given androgenetic individual,indicating that segregation of chromosome fragments is active from the early cell divisions. Most of the fragments were telomereless, i.e. they had no telomeric sequences on their ends. This shows that telomeres are not necessary for stability of chromosomal structures in a vertebrate genome. In one individual, the interstitial telomeric sites were found in chromosomes, which could be the effect of joining chromosome fragments.

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Section: Discussionmentioning

confidence: 99%

The stability of telomereless chromosome fragments in adult androgenetic rainbow trout

Ocalewicz

Babiak

Dobosz

et al. 2004

Journal of Experimental Biology

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“…But this study did not reveal the DNA sequences of the microchromosomes. Peek AS et al [2] found the paternal genetic material in the gynogenetic albino rainbow trout ( Oncorhynchus mykiss ) activated by the UV-treated sperm of the wild type pigmented brook trout ( Salvelinus fontinalis ). However, the knowledge regarding the effects of the heterogenous sperm in the gynogenesis is little.…”

Section: Introductionmentioning

confidence: 99%

Evidence for paternal DNA transmission to gynogenetic grass carp

Mao

Wang

et al. 2019

BMC Genet

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BackgroundGrass carp (Ctenopharyngodon idellus, GC), as the highest-output fish in China, is economically important. The production of gynogenetic grass carp (GGC) will provide important germplasm resource for producing improved GC. At present, knowledge regarding the heterologous sperm DNA in gynogenetic offspring is little. Thus, revealing paternal DNA in GGC at the molecular level would be highly significant for fish genetic breeding.ResultIn this study, ultraviolet-treated sperm of koi carp (Cyprinus carpio haematopterus, KOC, 2n = 100), was used to activate the eggs of GC (2n = 48). Afterwards, cold shock (0–4 °C) was administered for 12 min to double the chromosomes, resulting in GGC. No significant difference (p > 0.05) was found between GGC and GC in appearance, erythrocytes size and chromosome numbers. However, at the molecular level, a specific microsatellite DNA fragment (MFW1-gynogenetic grass carp, MFW1-G) derived from the paternal parent KOC was found to be transmitted into GGC.ConclusionsFor the first time, this study provided an evidence at the molecular level that the DNA fragment derived from the paternal parent occurred in GGC. This finding is of great significance for fish genetic breeding.

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“…Karyotyping in rainbow trout (O. mykiss) showed residual paternal inheritance in gynogenetic offspring (Thorgaard et al, 1985). These findings and techniques were used to incorporate a paternal minichromosome in gynogenetic offspring (Peek et al, 1997).…”

Section: Discussionmentioning

confidence: 94%

“…Nevertheless, with this data set it is impossible to infer or determine how paternal fragments were maintained. Other studies suggest that paternal residuals can persist by several mechanisms, including random genomic integration (Chen et al ., 2020) and propagation as chromosomal fragments (Ocalewicz et al ., 2004; Peek et al ., 1997; Thorgaard et al ., 1985; Yi et al ., 2003). The paternal markers identified in this study were predominantly not tightly linked, leading the authors to infer they could be borne on small fragments that might be missed with standard karyotypic methods, such as those used in Nanda et al .…”

Section: Discussionmentioning

confidence: 99%

“…Karyotyping in rainbow trout ( O. mykiss ) showed residual paternal inheritance in gynogenetic offspring (Thorgaard et al ., 1985). These findings and techniques were used to incorporate a paternal minichromosome in gynogenetic offspring (Peek et al ., 1997). In studies of androgenetic rainbow trout, maternal chromosomal fragments were found in karyotyped‐treated offspring (Ocalewicz et al ., 2004).…”

Section: Discussionmentioning

confidence: 99%

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Genome‐wide analysis facilitates estimation of the amount of male contribution in meiotic gynogenetic three‐spined stickleback (Gasterosteus aculeatus)

Currey

Walker

Bassham

et al. 2023

Journal of Fish Biology

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Gynogenetic embryosthose inheriting only maternal DNAcan be experimentally created by fertilizing eggs with radiation-treated sperm containing inactivated paternal chromosomes. Diploidy in the zygotes can be maintained through prevention of the second meiosis or restored by preventing the first mitosis after the maternal chromosome complement has been replicated. These gynogenetic organisms are useful in many fields including aquaculture, evolutionary biology and genomics. Although gynogenetic organisms have been created in numerous species, the completeness of uni-parental inheritance has often been assumed rather than thoroughly quantified across the genome. Instead, when tests of uni-parental inheritance occur, they typically rely on well-studied genetically determined phenotypes that represent a very small sub-set of the genome. Only assessing small genomic regions for paternal inheritance leaves the question of whether some paternal contributions to offspring might still have occurred. In this study, the authors quantify the efficacy of creating gynogenetic diploid three-spined stickleback fish (Gasterosteus aculeatus). To this end, the authors mirrored previous assessments of paternal contribution using well-studied genetically determined phenotypes including sex and genetically dominant morphological traits but expanded on previous studies using dense restriction site-associated DNA sequencing (RAD-seq) markers in parents and offspring to assess paternal inheritance genome-wide. In the gynogenetic diploids, the authors found no male genotypes underlying their phenotypes of interestsex and dominant phenotypic traits. Using genome-wide assessments of paternal contribution, nevertheless, the authors found evidence of a small, yet potentially important, amount of paternally "leaked" genetic material. The application of this genome-wide approach identifies the need for more widespread assessment of paternal contributions to gynogenetic animals and promises benefits for many aspects of aquaculture, evolutionary biology and genomics.

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A minichromosome carrying a pigmentation gene and brook trout DNA sequences in transgenic rainbow trout

Cited by 12 publications

References 39 publications

The stability of telomereless chromosome fragments in adult androgenetic rainbow trout

The stability of telomereless chromosome fragments in adult androgenetic rainbow trout

Evidence for paternal DNA transmission to gynogenetic grass carp

Genome‐wide analysis facilitates estimation of the amount of male contribution in meiotic gynogenetic three‐spined stickleback (Gasterosteus aculeatus)

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