A Mini-Review: Clinical Development and Potential of Aptamers for Thrombotic Events Treatment and Monitoring

Ponce, Alex; Hong, Ka Lok

doi:10.3390/biomedicines7030055

Cited by 29 publications

(23 citation statements)

References 63 publications

(102 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Considering its pivotal role in the coagulation cascade, the inhibition of thrombin activity is one of the most efficient antithrombotic strategies [ 89 , 90 , 91 , 92 ]. In this context, the development of effective antithrombin aptamers has been the focus of several investigations [ 92 , 93 , 94 , 95 , 96 ].…”

Section: Anticoagulant Aptamersmentioning

confidence: 99%

Dimeric and Multimeric DNA Aptamers for Highly Effective Protein Recognition

et al. 2020

View full text Add to dashboard Cite

Multivalent interactions frequently occur in biological systems and typically provide higher binding affinity and selectivity in target recognition than when only monovalent interactions are operative. Thus, taking inspiration by nature, bivalent or multivalent nucleic acid aptamers recognizing a specific biological target have been extensively studied in the last decades. Indeed, oligonucleotide-based aptamers are suitable building blocks for the development of highly efficient multivalent systems since they can be easily modified and assembled exploiting proper connecting linkers of different nature. Thus, substantial research efforts have been put in the construction of dimeric/multimeric versions of effective aptamers with various degrees of success in target binding affinity or therapeutic activity enhancement. The present review summarizes recent advances in the design and development of dimeric and multimeric DNA-based aptamers, including those forming G-quadruplex (G4) structures, recognizing different key proteins in relevant pathological processes. Most of the designed constructs have shown improved performance in terms of binding affinity or therapeutic activity as anti-inflammatory, antiviral, anticoagulant, and anticancer agents and their number is certainly bound to grow in the next future.

show abstract

Section: Anticoagulant Aptamersmentioning

confidence: 99%

Dimeric and Multimeric DNA Aptamers for Highly Effective Protein Recognition

et al. 2020

View full text Add to dashboard Cite

show abstract

“…26 nükleotid uzunluğunda olan aptamer faktör IIa'yı hedefleyerek iş görmektedir 31,32 . Pıhtılaşmanın engellenmesi için Faz çalışmaları dışında klinik öncesi geliştirilme aşamasında bulunan çeşitli aptamerler de tasarlanmıştır 33 .…”

Section: Trombozunclassified

Terapötik Aptamerler

Kılıçkaya¹

2019

jamp

View full text Add to dashboard Cite

Aptamerler kısa tek zincirli ve nükleik asit yapısında olan, SELEX (systematic evolution of ligands by exponential enrichment) adı verilen bir teknik ile geliştirilen ve hedeflerine yüksek ilgi ve özgüllük ile bağlanabilen moleküllerdir. Sentezlenmelerinin kolaylığı, yapılarının küçüklüğü ve biyolojik uyumları sayesinde hem tanı hem de tedavi alanında kullanılabilecek aday moleküllerdir. Bu kısa derlemede piyasada müstahzarı olan ve çeşitli Faz aşamalarında bulunan terapötik aptamerler hakkında bilgi verilmiştir.

show abstract

“…Aptamers are short nucleotide oligomers selected and isolated by the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) technique [ 1 , 2 , 3 , 4 ] to present high specificity and affinity for a large variety of targets ranging from ions [ 5 , 6 , 7 , 8 ] and small molecules [ 9 , 10 , 11 , 12 ] to whole cells [ 13 , 14 , 15 ]. Their ability to selectively recognize and bind targets recommends them as promising alternatives to antibodies for scientific and medical purposes [ 9 , 13 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 ]. Aptamers have been investigated as potential analytical and diagnostic tools with approaches and technologies that traditionally employ antibodies as recognition elements.…”

Section: Introductionmentioning

confidence: 99%

Kinetic Exclusion Assay of Biomolecules by Aptamer Capture

Smith

Fologea

2020

Sensors

View full text Add to dashboard Cite

DNA aptamers are short nucleotide oligomers selected to bind a target ligand with affinity and specificity rivaling that of antibodies. These remarkable features recommend aptamers as candidates for analytical and therapeutic applications that traditionally use antibodies as biorecognition elements. Numerous traditional and emerging analytical techniques have been proposed and successfully implemented to utilize aptamers for sensing purposes. In this work, we exploited the analytical capabilities offered by the kinetic exclusion assay technology to measure the affinity of fluorescent aptamers for their thrombin target and quantify the concentration of analyte in solution. Standard binding curves constructed by using equilibrated mixtures of aptamers titrated with thrombin were fitted with a 1:1 binding model and provided an effective Kd of the binding in the sub-nanomolar range. However, our experimental results suggest that this simple model does not satisfactorily describe the binding process; therefore, the possibility that the aptamer is composed of a mixture of two or more distinct Kd populations is discussed. The same standard curves, together with a four-parameter logistic equation, were used to determine “unknown” concentrations of thrombin in mock samples. The ability to identify and characterize complex binding stoichiometry, together with the determination of target analyte concentrations in the pM–nM range, supports the adoption of this technology for kinetics, equilibrium, and analytical purposes by employing aptamers as biorecognition elements.

show abstract

A Mini-Review: Clinical Development and Potential of Aptamers for Thrombotic Events Treatment and Monitoring

Cited by 29 publications

References 63 publications

Dimeric and Multimeric DNA Aptamers for Highly Effective Protein Recognition

Dimeric and Multimeric DNA Aptamers for Highly Effective Protein Recognition

Terapötik Aptamerler

Kinetic Exclusion Assay of Biomolecules by Aptamer Capture

Contact Info

Product

Resources

About