A mild, rapid, and efficient method of lipid extraction for use in determining vitamin E/lipid ratios

Burton, Graham W.; Webb, Ann; Ingold, K. U.

doi:10.1007/bf02534359

Cited by 377 publications

(147 citation statements)

References 38 publications

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“…The vitamin E content of the feeds was determined by the method of Pocklington and Dieffenbacher (1988). Vitamin E was extracted from plasma by the method of Burton et al (1985). A modified procedure from that described by Liu et al (1996) was employed to extract vitamin E from m. semimembranosus, liver and adipose tissue samples, but with 1 g of tissue and proportionate changes in volumes of reagents.…”

Section: Methodsmentioning

confidence: 99%

Influence of vitamin E supplementation and basal diet on the vitamin E status, performance and tissue fatty acid concentration in lambs

Kasapidou

Enser

Wood

et al. 2009

Animal

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In order to determine the effect of dietary vitamin E level and basal diet on vitamin E status, performance and tissue fatty acid content, five groups of eight Suffolk 3 Charollais wether lambs with an initial live weight of 28.4 (s.d. 1.6) kg were allocated to one of five concentrate-based diets supplemented with all-rac-a-tocopheryl acetate to contain 30 mg (C-30), 60 mg (C-60), 120 mg (C-120), 250 mg (C-250) or 500 mg (C-500) a-tocopheryl acetate/kg dry matter (DM), for 63 days. Two additional groups of eight lambs entered the study at 31.2 (s.d. 3.3) kg and were fed grass silage and 400 g/day concentrate for 56 days, with the whole diet providing the equivalent of 60 mg (S-60) or 500 mg (S-500) a-tocopheryl acetate/kg DM. Lambs were weighed and blood samples obtained by venipuncture weekly. Dietary vitamin E level did not affect performance ( P . 0.05), but lambs fed grass silage grew more slowly ( P , 0.001) and had a higher ( P , 0.001) feed conversion ratio (kg feed/kg gain) than those fed concentrates. At day 0 plasma a-tocopherol concentrations were 0.8 mg/ml and did not differ between treatments ( P . 0.05). Plasma a-tocopherol concentrations then decreased in all lambs except for those fed S-500, which increased, and at slaughter were (mg/ml) 0.07, 0.23, 0.39, 0.76 and 1.57 in C-30, C-60, C-120, C-250 and C-500 and 1.18 and 1.93 in S-60 and S-500, respectively. At slaughter, muscle and liver a-tocopherol concentrations were in the deficiency range for lambs fed C-30, C-60 or C-120, whereas plasma creatine kinase and tissue polyunsaturated fatty acids were unaffected by dietary vitamin E level, but creatine kinase levels were higher ( P , 0.05) and glutathione peroxidise levels lower ( P , 0.001) in lambs fed grass silage than concentrates alone. Muscle and liver a-tocopherol concentrations were 1.8-and 4.1-fold higher in lambs fed S-60 than C-60, but there was less of a difference between lambs fed S-500 or C-500 with muscle and liver differences of 0.4-and 0.7-fold, respectively. Tissue n-3 polyunsaturated fatty acid concentrations were higher ( P , 0.05) and n-6 fatty acids lower in lambs receiving the grass silage compared to concentrate-based diets, but were not affected by dietary vitamin E level. It is concluded that lower plasma and tissue levels of a-tocopherol are present in lambs supplemented with all-rac-a-tocopheryl acetate on a concentrate compared to a mixed diet of silage and concentrates, and that normal growth can be achieved at tissue levels previously considered to represent deficiency.Keywords: a-tocopherol, concentrates, fatty acids, grass silage, lamb growth ImplicationsVitamin E is necessary to protect tissues from oxidative damage. When lambs were fed a concentrate or grass silage-based diet supplemented with increasing amounts of vitamin E, substantially lower plasma and tissue vitamin E levels were observed in lambs receiving the concentratebased diets. Animal performance was, however, unaffected by dietary vitamin E level. Tissue fatty acid composition was also unaffected by...

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Section: Methodsmentioning

confidence: 99%

Influence of vitamin E supplementation and basal diet on the vitamin E status, performance and tissue fatty acid concentration in lambs

Kasapidou

Enser

Wood

et al. 2009

Animal

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“…The extraction and separation of endogenous membrane-containing vitamin E was performed by following the methods described by Vatassery et al (1978), Leenheer et al (1979) and Burton et al (1985). To 0.5 ml of the cell suspension, 1.5 ml 10 mM SDS was added, followed by addition of 2 ml ethanol.…”

Section: Retinal Cell Content In 6itamin Ementioning

confidence: 99%

Influence of vitamin E succinate on retinal cell survival

et al. 1998

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In this study, we analyzed the influence of vitamin E succinate (5 -80 vM), supplemented in the culture medium, on the survival of cultured retinal cells. The release of lactate dehydrogenase (LDH) was decreased in the presence of low concentrations (10-20 vM) of vitamin E succinate, whereas high concentrations (80 vM) induced a significant increase (about 2-fold) in the release of LDH, indicating a reduction of plasma membrane integrity. Supplementing with vitamin E succinate (80 vM) greatly enhanced its cellular content, as compared to vitamin E acetate (80 vM), and the membrane order of the retinal cells, as evaluated by the fluorescence anisotropy (r) of TMA-DPH (1-(4-(trimethylammonium)-phenyl)-6-phenylhexa-1,3,5-triene), was not altered. Furthermore, vitamin E succinate was more potent than vitamin E acetate in reducing thiobarbituric acid reactive substances (TBARS) formation upon ascorbate-Fe 2 + -induced oxidative stress (TBARS formation after cell oxidation decreased by about 15-fold or 1.6 fold, respectively, in the presence of 20 vM vitamin E succinate or 20 vM vitamin E acetate). A decrease in MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) reduction induced by supplementing with vitamin E succinate (80 vM), to 35.99 91.96% as compared to the control, but not by vitamin E acetate (80 vM), suggests that vitamin E succinate may affect the mitochondrial activity. Vitamin E succinate also reduced significantly the ATP:ADP ratio in a dose-dependent manner, indicating that vitamin E succinate-mediated cytotoxic effects involve a decrement of mitochondrial function.

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“…To measure ~-tocopherol, it was extracted from the membrane by heptane according to the method of Burton et al [11]. cz-Tocopherol was quantified using HPLC (C18 nucleosil column; Chrompack, The Netherlands; eluted with 98% methanol, flow rate 0.6 ml/min) with fluorometric detection (excitation 295 nm, emission 340 nm).…”

Section: Measurement Of Cz-tocopherolmentioning

confidence: 99%

The widely used anesthetic agent propofol can replace α‐tocopherol as an antioxidant

et al. 1995

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The cell membrane is protected against lipid peroxidation through endogenous antioxidants such as the lipid soluble a-tocopherol. The anesthetic agent propofol (2,6-diisopropylphenol) has a chemical structure which is similar to ot-tocopherol, since it also contains a phenolic OH-group. The transient protection of GSH against lipid peroxidation in control liver microsomes is not observed in microsomes deficient in a-tocopherol. Introducing propofol (2 and 5 tiM) restored the protective effect of GSH. Similar to the control microsomes the GSH-protective effect did not occur in previously heated microsomes. These results suggest that propofol acts similarly to a-tocopherol as a chain breaking antioxidant in liver microsomal membranes.

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A mild, rapid, and efficient method of lipid extraction for use in determining vitamin E/lipid ratios

Cited by 377 publications

References 38 publications

Influence of vitamin E supplementation and basal diet on the vitamin E status, performance and tissue fatty acid concentration in lambs

Influence of vitamin E supplementation and basal diet on the vitamin E status, performance and tissue fatty acid concentration in lambs

Influence of vitamin E succinate on retinal cell survival

The widely used anesthetic agent propofol can replace α‐tocopherol as an antioxidant

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