A microwave matrix sensor for multipoint label-free Escherichia coli detection

Piekarz, Ilona; Górska, Sabina; Odrobina, Szczepan; Drab, Marek; Wincza, Krzysztof; Gamian, Andrzej; Gruszczyński, Sławomir

doi:10.1016/j.bios.2019.111784

Cited by 25 publications

(15 citation statements)

References 34 publications

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“…While simplistic in design, microcentrifuging at forces high enough for pathogen separation is technically difficult, making it problematic for POCC applications where technologies should be simplistic and streamlined (Cihalova et al, 2017; Nguyen et al, 2019; Yoo et al, 2011). Most recently, Piekarz et al developed a 25 array microwave Escherichia coli detection system which uses antibody‐functionalized electrodes and measures capacitance change from bacterial load, estimating a 10 3 CFU/ml LOD (Piekarz et al, 2020). When studying both bacteria and fungi, Lehmann et al developed a multiplexed PCR system which can differentiate 25 pathogens from whole blood samples in under 6 h. Here, gram‐positive, gram‐negative, and fungi pathogens are developed and analyzed in parallel, and has over 98% specificity between samples (Lehmann et al, 2008).…”

Section: M/n Technologies For Sepsis Biomarkersmentioning

confidence: 99%

Point‐of‐critical‐care diagnostics for sepsis enabled by multiplexed micro and nanosensing technologies

Ashley

Hassan

2021

WIREs Nanomed Nanobiotechnol

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Sepsis is responsible for the highest economic and mortality burden in critical care settings around the world, prompting the World Health Organization in 2018 to designate it as a global health priority. Despite its high universal prevalence and mortality rate, a disproportionately low amount of sponsored research funding is directed toward diagnosis and treatment of sepsis, when early treatment has been shown to significantly improve survival. Additionally, current technologies and methods are inadequate to provide an accurate and timely diagnosis of septic patients in multiple clinical environments. For improved patient outcomes, a comprehensive immunological evaluation is critical which is comprised of both traditional testing and quantifying recently proposed biomarkers for sepsis. There is an urgent need to develop novel point‐of‐care, low‐cost systems which can accurately stratify patients. These point‐of‐critical‐care sensors should adopt a multiplexed approach utilizing multimodal sensing for heterogenous biomarker detection. For effective multiplexing, the sensors must satisfy criteria including rapid sample to result delivery, low sample volumes for clinical sample sparring, and reduced costs per test. A compendium of currently developed multiplexed micro and nano (M/N)‐based diagnostic technologies for potential applications toward sepsis are presented. We have also explored the various biomarkers targeted for sepsis including immune cell morphology changes, circulating proteins, small molecules, and presence of infectious pathogens. An overview of different M/N detection mechanisms are also provided, along with recent advances in related nanotechnologies which have shown improved patient outcomes and perspectives on what future successful technologies may encompass. This article is categorized under: Diagnostic Tools > Biosensing

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Section: M/n Technologies For Sepsis Biomarkersmentioning

confidence: 99%

Point‐of‐critical‐care diagnostics for sepsis enabled by multiplexed micro and nanosensing technologies

Ashley

Hassan

2021

WIREs Nanomed Nanobiotechnol

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“…These properties play an important role in cell life and are mediated by the presence of water, macromolecules dissolved in it, as well as the localization of intracellular structures of prokaryotes and eukaryotic organisms. Moreover, the dielectric properties of cells underlie intercellular interaction in complex multicellular biological systems or in bacterial populations, and therefore are unique and specific to every living organism [69,107,108].…”

Section: Microwave Label-free Biosensorsmentioning

confidence: 99%

“…Therefore, the permittivity of bacterial cells on the influence of external electric fields depends on their species, type (gram-positive or gram-negative) [107,109,110], as well as on the electrical conductivity and permittivity of various intracellular components of bacteria. In addition, they may depend on the physiological state of bacteria (metabolic activity level, degree of viability), as well as on living conditions and the state of their hydration [111][112][113].…”

Section: Microwave Label-free Biosensorsmentioning

confidence: 99%

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Label-Free Biosensors for Laboratory-Based Diagnostics of Infections: Current Achievements and New Trends

et al. 2020

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Infections pose a serious global public health problem and are a major cause of premature mortality worldwide. One of the most challenging objectives faced by modern medicine is timely and accurate laboratory-based diagnostics of infectious diseases. Being a key factor of timely initiation and success of treatment, it may potentially provide reduction in incidence of a disease, as well as prevent outbreak and spread of dangerous epidemics. The traditional methods of laboratory-based diagnostics of infectious diseases are quite time- and labor-consuming, require expensive equipment and qualified personnel, which restricts their use in case of limited resources. Over the past six decades, diagnostic technologies based on lateral flow immunoassay (LFIA) have been and remain true alternatives to modern laboratory analyzers and have been successfully used to quickly detect molecular ligands in biosubstrates to diagnose many infectious diseases and septic conditions. These devices are considered as simplified formats of modern biosensors. Recent advances in the development of label-free biosensor technologies have made them promising diagnostic tools that combine rapid pathogen indication, simplicity, user-friendliness, operational efficiency, accuracy, and cost effectiveness, with a trend towards creation of portable platforms. These qualities exceed the generally accepted standards of microbiological and immunological diagnostics and open up a broad range of applications of these analytical systems in clinical practice immediately at the site of medical care (point-of-care concept, POC). A great variety of modern nanoarchitectonics of biosensors are based on the use of a broad range of analytical and constructive strategies and identification of various regulatory and functional molecular markers associated with infectious bacterial pathogens. Resolution of the existing biosensing issues will provide rapid development of diagnostic biotechnologies.

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“…Despite much research being devoted to the detection of E. coli , it still has disadvantages such as costly, time‐consuming, culturing methods, and colony counting. In particular, many researchers are developing label‐free detection methods to reduce the time consumed by the labeling process, which generally takes more than 1 day after incubation 13–16 …”

Section: Introductionmentioning

confidence: 99%

Amphiphilic Conjugated Polythiophene‐based Fluorescence “Turn on” Sensor for Selective Detection of Escherichia coli in Water and Milk

Shim

Tawfik

Thangadurai³

et al. 2021

Bulletin Korean Chem Soc

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The ability to detect pathogenic microbes in water and food samples is of prominent interest, but it remains a great challenge. Herein, we report a simple fluorescence sensor based on amphiphilic conjugated polythiophene (ACPT) for the detection of gram-negative pathogenic bacteria, Escherichia coli, in pH 7.0. The ACPT sensor was synthesized by the oxidative polymerization of water-soluble thiophene monomers in an aqueous solution. The ACPT sensor undergoes the fluorescence "turn on" response at 432 nm upon the addition of E. coli. The ionic complex, which is generated by electrostatic interaction during the short incubation time of 15 min, resulting in the enhancement of the fluorescence intensity of ACPT. The application of ACPT enables the detection of E. coli pathogens in tap water and milk samples with good recoveries of 96.94-100.83% and 93.27-116.75%, respectively, and excellent reproducibility (n = 3, relative standard deviation [RSD] = 1.78%). The current findings pave the way for the promising application of a novel ACPT system that could be used in food safety and environmental monitoring.

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A microwave matrix sensor for multipoint label-free Escherichia coli detection

Cited by 25 publications

References 34 publications

Point‐of‐critical‐care diagnostics for sepsis enabled by multiplexed micro and nanosensing technologies

Point‐of‐critical‐care diagnostics for sepsis enabled by multiplexed micro and nanosensing technologies

Label-Free Biosensors for Laboratory-Based Diagnostics of Infections: Current Achievements and New Trends

Amphiphilic Conjugated Polythiophene‐based Fluorescence “Turn on” Sensor for Selective Detection of Escherichia coli in Water and Milk

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