A microwave-assisted fluorescent labeling method for the separation and detection of amphetamine-like designer drugs by capillary electrophoresis

Chen, Kuan Fu; Lee, Hsun; Liu, Ju Tsung; Lee, Huan An; Lin, Cheng Huang

doi:10.1016/j.forsciint.2013.02.045

Cited by 13 publications

(7 citation statements)

References 31 publications

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“…Comparable results in terms of sensitivity were obtained by conventional convective heating but with longer reaction times (120 times). These results are consistent with literature, demonstrating that microwaves can be very efficient for remarkably accelerating fluorescent tagging [37,48]. As for conventional convective heating, blank assays conducted (Section 2.5) confirmed that MW heating had low effect on FITC stability.…”

Section: Labeling Of Amino Acids With Fluorescein Isothiocyanate Isomsupporting

confidence: 89%

Microalgae amino acid extraction and analysis at nanomolar level using electroporation and capillary electrophoresis with laser‐induced fluorescence detection

Nehmé

Atieh

Fayad

et al. 2016

J of Separation Science

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Amino acids play a key role in food analysis, clinical diagnostics, and biochemical research. Capillary electrophoresis with laser-induced fluorescence detection was used for the analysis of several amino acids. Amino acid labeling with fluorescein isothiocyanate was conducted using microwave-assisted derivatization at 80°C (680 W) during only 150 s. Good electrophoretic resolution was obtained using a background electrolyte composed of sodium tetraborate buffer (100 mM; pH 9.4) and β-cyclodextrin (10 mM), and the limits of quantification were 3-30 nM. The developed capillary electrophoresis with laser-induced fluorescence method was used to analyze amino acids in Dunaliella salina green algae grown under different conditions. A simple extraction technique based on electroporation of the cell membrane was introduced. A home-made apparatus allowed the application of direct and alternating voltages across the electrochemical compartment containing a suspension of microalgae in distilled water at 2.5 g/L. A direct voltage of 12 V applied for 4 min gave the optimum extraction yield. Results were comparable to those obtained with accelerated-solvent extraction. The efficiency of electroporation in destroying microalgae membranes was shown by examining the algae surface morphology using scanning electron microscopy. Stress conditions were found to induce the production of amino acids in Dunaliella salina cells.

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Section: Labeling Of Amino Acids With Fluorescein Isothiocyanate Isomsupporting

confidence: 89%

Microalgae amino acid extraction and analysis at nanomolar level using electroporation and capillary electrophoresis with laser‐induced fluorescence detection

Nehmé

Atieh

Fayad

et al. 2016

J of Separation Science

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“…Amphetamines are generally assessed by urine analysis. Many methods have been reported to assess amphetamines compounds in human urine samples using gas chromatography (GC) [3], high-performance liquid chromatography (HPLC) [4,5], GC/mass spectrometry (MS) [6][7][8][9][10][11][12], HPLC-MS [13,14], capillary electrophoresis (CE) [15], and CE-MS [16]. GC and GC-MS methods are often preferred for quantitative determination of ecstasy and amphetamines compounds.…”

Section: Introductionmentioning

confidence: 99%

Solid-phase extraction followed by dispersive liquid-liquid microextraction for the sensitive determination of ecstasy compounds and amphetamines in biological samples

Mashayekhi¹,

Rezaee²,

Khalilian³

2014

Bull. Chem. Soc. Eth.

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ABSTRACT. A novel approach for the determination of ecstasy and amphetamines (3,4-methylenedioxymethylamphetamine (MDMA, Ecstasy), 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxyethylamphetamine (MDEA) and 3,4-methylenedioxypropylamphetamine (MDPA)) in biological samples is presented. The analytes were extracted from the matrix and transferred to a small volume of a high density, water insoluble solvent using solid-phase extraction (SPE) followed by dispersive liquid-liquid microextraction (DLLME). This combination not only resulted in a high enrichment factor, but also it could be used in complex matrices (biological samples). Some important extraction parameters, such as sample solution flow rate, sample pH, type and volume of extraction and disperser solvents as well as the salt addition, were studied and optimized. Under the optimized conditions, the calibration graphs were linear in the range of 0.5-500 µg L -1 and 1.0-500 µg L -1 with detection limits in the range of 0.1-0.3 µg L -1 and 0.2-0.7 µg L -1 in urine and plasma samples, respectively. The results showed that SPE-DLLME is a suitable method for the determination of ecstasy components and amphetamines in biological and water samples.

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“…где S λ -аналитический сигнал на длине волны λ, D λтемновой сигнал на длине волны λ, R λ -опорный сигнал на длине волны λ. Спектры каждой пробы экспортировали в виде табличных данных в программу Origin TM (OriginLab Corp., США) для последующего анализа. ствовало литературным данным [20,21], и отсутствие поглощения при длине волны более 560 nm (рис. 1, а, 2, а).…”

Section: экспериментальные измеренияunclassified

Спектрофотометрическая Характеристика Конъюгатов Иммуноглобулинов Для Диагностики Возбудителей Особо Опасных Инфекций

Spitsyn¹,

Уткин²,

Kireev³

et al. 2020

Журнал Технической Физики

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The possibility of using spectrophotometric analysis to characterize fluorescent immunoglobulins as a control method was considered. A comparative analysis of the optical properties of fluorescent immunoglobulin preparations and their constituent components - immunoglobulins, fluorochrome was carried out. The results suggest that the proposed methodological approach of optical detection of labeled immunoglobulin molecules is promising for controlling the preparation of conjugates used in the formulation of immunological reactions to identify specific antigens of pathogens of particularly dangerous infections.

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A microwave-assisted fluorescent labeling method for the separation and detection of amphetamine-like designer drugs by capillary electrophoresis

Cited by 13 publications

References 31 publications

Microalgae amino acid extraction and analysis at nanomolar level using electroporation and capillary electrophoresis with laser‐induced fluorescence detection

Microalgae amino acid extraction and analysis at nanomolar level using electroporation and capillary electrophoresis with laser‐induced fluorescence detection

Solid-phase extraction followed by dispersive liquid-liquid microextraction for the sensitive determination of ecstasy compounds and amphetamines in biological samples

Спектрофотометрическая Характеристика Конъюгатов Иммуноглобулинов Для Диагностики Возбудителей Особо Опасных Инфекций

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