A microvolumetric blood counter/sampler for metabolic PET studies in small animals

Lapointe, D.; Cadorette, J.; Rodrigue, S.; Rouleau, D.

doi:10.1109/23.708343

Cited by 22 publications

(12 citation statements)

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“…Furthermore, the initial investigation of new radiotracers requires direct and accurate determination of plasma and metabolite concentration of the tracer as a function of time (27). The proposed flow-through whole-blood counter allows an automated blood sampler to be inserted on the same withdrawing line (11).…”

Section: Discussionmentioning

confidence: 99%

“…Like PET scanners, most external online counting systems are based on annihilation photon detection (11,28). Although this detection principle is very reliable, it requires complex electronics and bulky detectors that necessitate cumbersome shielding.…”

Section: Discussionmentioning

confidence: 99%

“…It was implemented in the control software as an 8-point curve defined between Dl min and Dl max with K opt 5 K(Dl min ). This protocol was repeated to obtain the optimum absolute sensitivity, K opt (cps/Bq), with the 4 most commonly used PET isotopes in our center ( 18 F, 13 N, 11 C, and 64 Cu) for PE50 and PE10 catheters. As the typical background count rate is negligible in most animal studies (14), these experiments were performed assuming a default background count rate arbitrarily set to 5 cps.…”

Section: Data Correctionsmentioning

confidence: 99%

“…Mini-PET systems over the wrist have been proposed for human studies, but the size and resolution of these devices is not adapted to small animals (9). More suitable external monitors for rodents were developed to address the problem (10)(11)(12)(13), but none of these devices provides an integrated solution for realtime measurement of blood radiotracer concentration that is fully adapted to rats and mice.…”

mentioning

confidence: 99%

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A New Tool for Molecular Imaging: The Microvolumetric Blood Counter

Convert¹,

Morin-Brassard²,

Cadorette³

et al. 2007

Journal of Nuclear Medicine

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Radiotracer kinetic modeling in small animals with PET allows absolute quantification of physiologic and biochemical processes in vivo. It requires blood and tissue tracer concentrations as a function of time. Manual sampling, the reference method for blood tracer concentration measurements, requires fairly large amounts of blood besides being technically difficult and timeconsuming. An automated microvolumetric b blood counter (mBC) was designed to circumvent these limitations by measuring the blood activity in real time with PET scanning. Methods: The mBC uses direct b-particle detection to reduce its footprint and is entirely remote controlled for sampling protocol selection and real-time monitoring of measured parameters. Sensitivity has been determined for the most popular PET radioisotopes ( 18 F, 13 N, 11 C, 64 Cu). Dispersion within the sampling catheter has been modeled to enable automatic correction. Blood curves obtained with the mBC were compared with manual samples and PET-derived data. The mBC was used to estimate the myocardial blood flow (MBF) of mice injected with 13 N-ammonia and to compare the myocardial metabolic rate of glucose (MMRG) of rats injected with 18 F-FDG for arterial and venous cannulation sites. Results: The sensitivity limit ranges from 3 to 104 Bq/mL, depending on the isotope and the catheter used, and was found to be adequate for most small-animal studies. Automatic dispersion correction appears to be a good approximation of dispersionfree reference curves. Blood curves sampled with the mBC are well correlated with curves obtained from manual samples and PET images. With correction for dispersion, the MBF of anesthetized mice at rest was found to be 4.84 6 0.5 mL/g/min, which is comparable to values found in the literature for rats. MMRG values derived from the venous blood tracer concentration are underestimated by 60% as compared with those derived from arterial blood. Conclusion: The mBC is a compact automated counter allowing real-time measurement of blood radioactivity for pharmacokinetic studies in animals as small as mice. Reliable and reproducible, the device makes it possible to increase the throughput of pharmacokinetic studies with reduced blood sample handling and staff exposure, contributing to speed up new drug development and evaluation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Data Correctionsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

A New Tool for Molecular Imaging: The Microvolumetric Blood Counter

Convert¹,

Morin-Brassard²,

Cadorette³

et al. 2007

Journal of Nuclear Medicine

Self Cite

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show abstract

“…Sin embargo, una rata tiene un volumen de sangre aproximado de 20 ml, de los cuales es posible extraer entre un 10 y un 12 % de forma segura durante un periodo de 60 a 90 minutos, que suele ser la duración de un experimento completo. Esta limitación del volumen de sangre a extraer dificulta las medidas, si bien algunos investigadores han diseñado ingeniosos métodos para poder llevarlas a cabo 44 . Las dificultades son aún mayores si el experimento se hace con ratones, dado que en ese caso apenas se puede disponer de 0,2 o 0,3 ml de sangre, de los aproximadamente 2,6 ml que tiene el animal.…”

Section: Limitaciones Experimentalesunclassified

Limitaciones tecnológicas de la tomografía por emisión de positrones (PET) para pequeños animales de laboratorio

Vaquero

Desco

2005

Revista Española de Medicina Nuclear

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Resumen.-La visualización y cuantificación de la función de determinados órganos en animales de laboratorio mediante PET está demostrando ser una herramienta de gran relevancia para la caracterización del fenotipo de animales transgénicos y noqueados, en el estudio de modelos de enfermedades humanas, así como para el descubrimiento y desarrollo de nuevos medicamentos y sondas bioquímicas. Para poder utilizar la PET en animales de laboratorio de un modo análogo al que se aplica en humanos es necesario contemplar el factor de escala en el tamaño del vóxel así como mantener una similar estadística de contaje. En este trabajo se apuntan los problemas que estos requerimientos representan tanto para el diseño técnico de los tomógrafos como para la realización de los experimentos, y desde esta perspectiva se analizan las soluciones tecnológi-cas más relevantes. Finalmente, se comentan brevemente algunas características de sistemas disponibles hoy comercialmente (microPET y FOCUS, HiDAC, eXplore VISTA, MOSAIC, YAP-(S)PET y rPET). TECHNICAL LIMITATIONS OF THE POSITRON EMISSION TOMOGRAPHY (PET) FOR SMALL LABORATORY ANIMALSAbstract.-Visualization and quantification of organ function by PET in small laboratory animals is becoming an outstanding tool for characterization of phenotype of transgenic and knock-out animals, for the study of animal models of human diseases, and for the development of new therapeutic drugs and diagnostic biochemical probes. To be able to make use of the PET with small laboratory animals in the same way as it is operated with humans it is necessary to account for the volumetric scale factor as well as for the requirement of maintaining the counting statistics. This work sketches the problems that these requirements represent for the technical design of the scanners and for the execution of the experiments. Finally, some characteristics of commercially available scanners (microPET/ FOCUS, HiDAC, eXplore VISTA, MOSAIC, YAP-(S)PET and rPET) are briefly discussed. PRESENTACIÓNLos últimos avances en instrumentación para imagen médica han conducido al diseño y desarrollo de sistemas de imagen molecular especialmente dedicados a la obtención de imágenes de pequeños animales de laboratorio, imposibles de conseguir con la instrumentación diseñada para humanos 1,2 . Estos equipos se usan fundamentalmente para investigación traslacional en biología molecular y medicina; se da la circunstancia de que el mayor número de modelos de enfermedades humanas, tanto en neurología como en cardiología y oncología, se ha desarrollado sobre roedores, debido al buen conocimiento de estos modelos y su bajo coste 3 . La evolución de la genómica y de la biología molecular, así como la investigación con animales modificados genéticamente 4 ha impulsado el desarrollo de sistemas no invasivos de imagen para pequeños animales con el fin de poder realizar estudios longitudinales en periodos de tiempo relativamente largos, experimentos en los que es necesaria la supervivencia del animal 5,6 . Sin embargo la disponibilidad y el acces...

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Book Review

Mais

1999

Cell Biology International

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A microvolumetric blood counter/sampler for metabolic PET studies in small animals

Cited by 22 publications

References 7 publications

A New Tool for Molecular Imaging: The Microvolumetric Blood Counter

A New Tool for Molecular Imaging: The Microvolumetric Blood Counter

Limitaciones tecnológicas de la tomografía por emisión de positrones (PET) para pequeños animales de laboratorio

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