A microsensing system for the in vivo real-time detection of local drug kinetics

Ogata, Genki; Ishii, Yuya; Asai, Kai; Sano, Yamato; Nin, Fumiaki; Yoshida, Tsukihisa; Higuchi, Taiga; Sawamura, Seishiro; Ôta, T.; Hori, Katsuo; Maeda, Kazuya; Komune, Shizuo; Doi, Kunio; Takai, Madoka; Findlay, Ian; Kusuhara, Hiroyuki; Einaga, Yasuaki; Hibino, Hiroshi

doi:10.1038/s41551-017-0118-5

Cited by 70 publications

(69 citation statements)

References 62 publications

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“…The subtraction current began to rise around 40 s after the injection (right, top). LFP data were analyzed by fast Fourier transform and shown as a dynamic spectrum, indicating the suppression of neural activity (right, bottom) [ 91 ]. (D) A fully implantable multi-panel device for remote monitoring of paracetamol in vivo in a living animal .…”

Section: In Vivo Biosensing Technologies For Continuous Drug Monitorimentioning

confidence: 99%

Towards wearable and implantable continuous drug monitoring: A review

Bian

Zhu

Rong

et al. 2021

Journal of Pharmaceutical Analysis

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Continuous drug monitoring is a promising alternative to current therapeutic drug monitoring strategies and has strong potential to reshape our understanding of pharmacokinetic variability and to improve individualised therapy. This review highlights recent advances in biosensing technologies that support continuous drug monitoring in real time. We focus primarily on aptamer-based biosensors, wearable and implantable devices. Emphasis is given to the approaches employed in constructing biosensors. We pay attention to sensors’ biocompatibility, calibration performance, long-term characteristics stability and measurement quality. In the end, we discuss about the current challenges and issues to address in continuous drug monitoring to make it a promising, future tool for individualised therapy. The ongoing efforts are expected to result in fully integrated implantable drug biosensing technology. Thus, we may anticipate an era of advanced healthcare in which wearable and implantable biochips automatically adjust drug dosing in response to patient health conditions enabling the management of diseases and enhancing individualised therapy.

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Section: In Vivo Biosensing Technologies For Continuous Drug Monitorimentioning

confidence: 99%

Towards wearable and implantable continuous drug monitoring: A review

Bian

Zhu

Rong

et al. 2021

Journal of Pharmaceutical Analysis

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“…The temperature of the cochlea was additionally controlled by supplemental heat to the head from a lamp [34]. After tracheotomy, which was intended for the maintenance of spontaneous breathing, an anterior portion of the bulla was opened with a ventral approach to expose the basal turn of the cochlea [36]. Then, the tensor tympani muscle was cut with a sharp scalpel [33].…”

Section: Animal Preparation For In Vivo Experimentsmentioning

confidence: 99%

Characterisation of the static offset in the travelling wave in the cochlear basal turn

Ôta

Nin

Choi

et al. 2020

Pflugers Arch - Eur J Physiol

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In mammals, audition is triggered by travelling waves that are evoked by acoustic stimuli in the cochlear partition, a structure containing sensory hair cells and a basilar membrane. When the cochlea is stimulated by a pure tone of low frequency, a static offset occurs in the vibration in the apical turn. In the high-frequency region at the cochlear base, multi-tone stimuli induce a quadratic distortion product in the vibrations that suggests the presence of an offset. However, vibrations below 100 Hz, including a static offset, have not been directly measured there. We therefore constructed an interferometer for detecting motion at low frequencies including 0 Hz. We applied the interferometer to record vibrations from the cochlear base of guinea pigs in response to pure tones. When the animals were exposed to sound at an intensity of 70 dB or higher, we recorded a static offset of the sinusoidally vibrating cochlear partition by more than 1 nm towards the scala vestibuli. The offset's magnitude grew monotonically as the stimuli intensified. When stimulus frequency was varied, the response peaked around the best frequency, the frequency that maximised the vibration amplitude at threshold sound pressure. These characteristics are consistent with those found in the low-frequency region and are therefore likely common across the cochlea. The offset diminished markedly when the somatic motility of mechanosensitive outer hair cells, the force-generating machinery that amplifies the sinusoidal vibrations, was pharmacologically blocked. Therefore, the partition offset appears to be linked to the electromotile contraction of outer hair cells.

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“…After tracheotomy, which was conducted for the maintenance of spontaneous breathing, the animals were paralyzed by intravenous injection of vecuronium bromide (3 mg/kg) (Vecuronium for intravenous injection; Fuji Pharma, Japan) [26]. Subsequently, the animal was artificially ventilated with room air using a respirator (SN-408-7; Shinano Manufacturing, Japan) [27]. We stopped the ventilation during the measurements for 4 s to prevent the motion artifact.…”

Section: Animal Preparationmentioning

confidence: 99%

In vivo tomographic visualization of intracochlear vibration using a supercontinuum multifrequency-swept optical coherence microscope

Choi

Nin

Ôta

et al. 2019

Biomed. Opt. Express

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This study combined a previously developed optical system with two additional key elements: a supercontinuum light source characterized by high output power and an analytical technique that effectively extracts interference signals required for improving the detection limit of vibration amplitude. Our system visualized 3D tomographic images and nanometer scale vibrations in the cochlear sensory epithelium of a live guinea pig. The transverse-and axial-depth resolution was 3.6 and 2.7 µm, respectively. After exposure to acoustic stimuli of 21-25 kHz at a sound pressure level of 70-85 dB, spatial amplitude and phase distributions were quantified on a targeted surface, whose area was 522 × 522 μm 2 .

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A microsensing system for the in vivo real-time detection of local drug kinetics

Cited by 70 publications

References 62 publications

Towards wearable and implantable continuous drug monitoring: A review

Towards wearable and implantable continuous drug monitoring: A review

Characterisation of the static offset in the travelling wave in the cochlear basal turn

In vivo tomographic visualization of intracochlear vibration using a supercontinuum multifrequency-swept optical coherence microscope

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