A microsatellite linkage map of the blacklip abalone, Haliotis rubra

Abstract

There is considerable scope for genetic improvement of cultured blacklip abalone Haliotis rubra in Australia using molecular marker-assisted, selective-breeding practices. Such improvement is dependent on the availability of primary genetic resources, such as a genetic linkage map. This study presents a first-generation linkage map of H. rubra, containing 122 microsatellite markers typed in a single full-sib family. These loci mapped to 17 and 20 linkage groups for the male and female respectively, and when aligned, the consensus map represented 18 linkage groups. The male linkage map contained 102 markers (one unlinked) covering 621 cM with an average intermarker spacing of 7.3 cM, and the female map contained 98 markers (eight unlinked) covering 766 cM with an average intermarker spacing of 9.8 cM. Analysis of markers informative in both parents showed a significantly higher recombination rate in the female parent, with an average male-to-female recombination ratio of 1:1.45 between linked pairs of markers. This linkage map represents a significant advancement in the genetic resource available for H. rubra and provides a framework for future quantitative trait loci mapping and eventual implementation of marker-assisted selection.

A Consensus Microsatellite-Based Linkage Map for the Hermaphroditic Bay Scallop (Argopecten irradians) and Its Application in Size-Related QTL Analysis

“…In this study, the recombination rate in female parents was higher than the males, with an overall female-to-male ratio of 1.13∶1. This is consistent with higher female recombination rates and longer map lengths observed in other marine mollusks, such as the oyster ( Crassostrea gigas , [9], [58]) and the abalone ( Haliotis rubra , F∶M = 1.45∶1 [68]). Difference in recombination rate between sexes may result from various complex factors [69].…”

Molecular genetics to inform spatial management in benthic invertebrate fisheries: a case study using the Australian Greenlip Abalone

“…Loci were checked for evidence of linkage disequilibrium in genepop 4.2 (Raymond & Rousset ), and there was no evidence of linkage among any of the 15 loci screened, which was consistent with the known independent linkage groups of many of the loci (Baranski et al . ). We tested for null alleles using Microchecker (Van Oosterhout et al .…”

A consensus linkage map of the grass carp (Ctenopharyngodon idella) based on microsatellites and SNPs

“…Until last decade, dominant DNA markers such as AFLP and RAPD were used to construct linkage maps that are now replaced by co-dominant markers such as microsatellites and SNPs. Microsatellites have been extensively used in construction of genetic maps for some aquaculture fishes recently, e.g., brown trout, Salmo trutta [5], nile tilapia, Oreochromis niloticus [6], channel catfish, Ictalurus punctatus [7,8], Asian seabass, Lates calcarifer [9] and blacklip abalone, Haliotis rubra [10]. With the rapid development of DNA sequencing and genotyping technologies, single nucleotide polymorphism (SNP) [11], the most frequent polymorphism in genome, became the most favored DNA markers for linkage mapping and whole genome association studies in humans [12] and model organisms [13].…”