A microRNA, miR-101a, controls mammary gland development by regulating cyclooxygenase-2 expression

Tanaka, Tetsuya; Haneda, Shingo; Imakawa, Kazuhiko; Sakai, Senkiti; Nagaoka, Kentaro

doi:10.1016/j.diff.2008.10.001

Cited by 87 publications

(74 citation statements)

References 26 publications

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“…Despite the established role of a variety of extrinsic and intrinsic signals regulating mammary gland development (Howard and Gusterson 2000;Macias and Hinck 2012), a restricted number of studies have identified and validated miRNAs with a role in any aspect of normal mammary gland biology (Avril-Sassen et al 2009;Tanaka et al 2009;Ucar et al 2010;Yang et al 2010;Cui et al 2011;Le Guillou et al 2012), and even fewer have studied their function using transgenic animal models (Ucar et al 2010;Le Guillou et al 2012). Here we unveiled the miRNA cluster miR-424(322)/503 as an important regulator of the massive reorganization that occurs in the mammary epithelium after pregnancy.…”

Section: Discussionmentioning

confidence: 99%

“…miRNAs post-transcriptionally regulate gene expression mainly by targeting mRNAs for cleavage, translational repression, and mRNA destabilization. A very limited number of studies have identified and validated miRNAs with a role in any aspect of normal mammary gland biology (Avril-Sassen et al 2009;Tanaka et al 2009;Ucar et al 2010;Yang et al 2010;Cui et al 2011;Le Guillou et al 2012), and even fewer have studied their function in vivo (Ucar et al 2010;Le Guillou et al 2012). …”

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confidence: 99%

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The miR-424(322)/503 cluster orchestrates remodeling of the epithelium in the involuting mammary gland

et al. 2014

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The mammary gland is a very dynamic organ that undergoes continuous remodeling. The critical regulators of this process are not fully understood. Here we identify the microRNA cluster miR-424(322)/503 as an important regulator of epithelial involution after pregnancy. Through the generation of a knockout mouse model, we found that regression of the secretory acini of the mammary gland was compromised in the absence of miR-424(322)/503. Mechanistically, we show that miR-424(322)/503 orchestrates cell life and death decisions by targeting BCL-2 and IGF1R (insulin growth factor-1 receptor). Furthermore, we demonstrate that the expression of this microRNA cluster is regulated by TGF-b, a well-characterized regulator of mammary involution. Overall, our data suggest a model in which activation of the TGF-b pathway after weaning induces the transcription of miR-424(322)/503, which in turn down-regulates the expression of key genes. Here, we unveil a previously unknown, multilayered regulation of epithelial tissue remodeling coordinated by the microRNA cluster miR-424(322)/503.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

The miR-424(322)/503 cluster orchestrates remodeling of the epithelium in the involuting mammary gland

et al. 2014

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“…Thus, miR-21 regulates a large number of target proteins that may be involved in cellular survival, apoptosis, and cell invasiveness (Jazbutyte and Thum 2010). Tanaka et al (2009) reported that miR-101a controls mammary gland development in mice through alterations in the expression of Cox-2. Several miRs, such as miR-141, miR-200a, and miR-200b, were upregulated in fetal thymi post-DES exposure.…”

Section: Discussionmentioning

confidence: 99%

Exposure to Diethylstilbestrol during Pregnancy Modulates MicroRNA Expression Profile in Mothers and Fetuses Reflecting Oncogenic and Immunological Changes

et al. 2015

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Prenatal exposure to diethylstilbestrol (DES) is known to cause an increased susceptibility to a wide array of clinical disorders in humans. Previous studies from our laboratory demonstrated that prenatal exposure to DES induces thymic atrophy and apoptosis in the thymus. In the current study, we investigated if such effects on the thymus result from alterations in the expression of microRNA (miR). To that end, pregnant C57BL/6 mice who were exposed to DES and miR profiles in thymocytes of both the mother and fetuses on postnatal day 3 (gestation day 17) were studied. Of the 609 mouse miRs examined, we noted 59 altered miRs that were common for both mothers and fetuses, whereas 107 altered miRs were specific to mothers only and 101 altered miRs were specific to fetuses only. Upon further analyses in the fetuses, we observed that DES-mediated changes in miR expression may regulate genes involved in important functions, such as apoptosis, autophagy, toxicity, and cancer. Of the miRs that showed decreased expression following DES treatment, miR-18b and miR-23a were found to possess complementary sequences and binding affinity for 39 untranslated regions of the Fas ligand (FasL) and Fas, respectively. Transfection studies confirmed that DES-mediated downregulation of miR-18b and miR-23a led to increased FasL and Fas expression. These data demonstrated that prenatal DES exposure can cause alterations in miRs, leading to changes in the gene expression, specifically, miR-mediated increased expression in FasL and Fas causing apoptosis and thymic atrophy.

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“…As an aspect of the relationship with the microRNA-101, the prediction based on PicTar and TargetScan DNA analysis software suggest that COX-2 could be one direct target for miR-101 because miR-101 has a seed region, which is able to bind to the COX-2 mRNA 3-UTR. Such as miR-101 and COX-2 can control the development of mammary gland (Tanaka et al, 2009), colon cancer (Strillacci et al 2009) and prostate cancer (Hao et al, 2011). In recent years, studies have found that some cancer cells ,which have a high-expression of COX-2, their ability of the proliferation and metastasis have also been highly focused.…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-101 Inhibits Cell Proliferation, Invasion, and Promotes Apoptosis by Regulating Cyclooxygenase-2 in Hela Cervical Carcinoma Cells

Huang¹,

Chen²,

Shi³

et al. 2013

Asian Pacific Journal of Cancer Prevention

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A microRNA, miR-101a, controls mammary gland development by regulating cyclooxygenase-2 expression

Cited by 87 publications

References 26 publications

The miR-424(322)/503 cluster orchestrates remodeling of the epithelium in the involuting mammary gland

The miR-424(322)/503 cluster orchestrates remodeling of the epithelium in the involuting mammary gland

Exposure to Diethylstilbestrol during Pregnancy Modulates MicroRNA Expression Profile in Mothers and Fetuses Reflecting Oncogenic and Immunological Changes

MicroRNA-101 Inhibits Cell Proliferation, Invasion, and Promotes Apoptosis by Regulating Cyclooxygenase-2 in Hela Cervical Carcinoma Cells

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