A MicroRNA-Mediated Insulin Signaling Pathway Regulates the Toxicity of Multi-Walled Carbon Nanotubes in Nematode Caenorhabditis elegans

Zhao, Yunli; Jiao, Yang; Wang, Dayong

doi:10.1038/srep23234

Cited by 45 publications

(20 citation statements)

References 52 publications

(72 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Head thrash and body bend were used to reflect the locomotion behavior [ 35 ]. These endpoints were analyzed under dissecting microscope as described [ 36 , 37 ].…”

Section: Methodsmentioning

confidence: 99%

Identification of interneurons required for the aversive response of Caenorhabditis elegans to graphene oxide

Xiao¹,

Chen²,

Krasteva³

et al. 2018

J Nanobiotechnol

Self Cite

View full text Add to dashboard Cite

BackgroundSo far, how the animals evade the environmental nanomaterials is still largely unclear. In this study, we employed in vivo assay system of Caenorhabditis elegans to investigate the aversive behavior of nematodes to graphene oxide (GO) and the underlying neuronal basis.ResultsIn this assay model, we detected the significant aversive behavior of nematodes to GO at concentrations more than 50 mg/L. Loss-of-function mutation of nlg-1 encoding a neuroligin with the function in connecting pre- and post-synaptic neurons suppressed the aversive behavior of nematodes to GO. Moreover, based on the neuron-specific activity assay, we found that the NLG-1 activity in AIY or AIB interneurons was required for the regulation of aversive behavior to GO. The neuron-specific activities of NLG-1 in AIY or AIB interneurons were also required for the regulation of GO toxicity.ConclusionsUsing nlg-1 mutant as a genetic tool, we identified the AIY and AIB interneurons required for the regulation of aversive behavior to GO. Our results provide an important neuronal basis for the aversive response of animals to environmental nanomaterials.Electronic supplementary materialThe online version of this article (10.1186/s12951-018-0373-y) contains supplementary material, which is available to authorized users.

show abstract

“…Head thrash and body bend were used to reflect the locomotion behavior [ 35 ]. These endpoints were analyzed under dissecting microscope as described [ 36 , 37 ].…”

Section: Methodsmentioning

confidence: 99%

Identification of interneurons required for the aversive response of Caenorhabditis elegans to graphene oxide

Xiao¹,

Chen²,

Krasteva³

et al. 2018

J Nanobiotechnol

Self Cite

View full text Add to dashboard Cite

show abstract

“…The method for brood size was performed as described previously 73 , 74 . The number of offspring at all stages beyond the egg was counted.…”

Section: Methodsmentioning

confidence: 99%

Toxicity evaluation of Wanzhou watershed of Yangtze Three Gorges Reservoir in the flood season in Caenorhabditis elegans

Xiao

Zhao

Huang

et al. 2018

Sci Rep

Self Cite

View full text Add to dashboard Cite

Three Gorges Reservoir (TGR) in the upper stream of Yangtze River in China is a reservoir with the largest and the longest yearly water-level drop. Considering the fact that most of safety assessments of water samples collected from TGR region were based on chemical analysis, we here employed Caenorhabditis elegans to perform in vivo safety assessment of original surface water samples collected from TGR region in the flood season in Wanzhou, Chongqing. Among the examined five original surface water samples, only exposure to original surface water sample collected from backwater area could induce the significant intestinal ROS production, enhance the intestinal permeability, and decrease the locomotion behavior. Additionally, exposure to original surface water sample collected from backwater area altered the expressions of sod-2, sod-5, clk-1, and mev-1. Moreover, mutation of sod-2 or sod-5 was susceptible to the potential toxicity of original surface water sample collected from backwater area on nematodes. Together, our results imply that exposure to surface water sample from the backwater area may at least cause the adverse effects on intestinal function and locomotion behavior in nematodes.

show abstract

“…And the same group prove that graphene oxide can regulate miR-360 to decrease DNA damage-apoptosis signaling by targeting CEP-1 [33,34]. Also miR-355 could regulate MWCNTs toxicity by target daf-2 , the gene encodes for the insulin-like growth factor 1 [35] (Table 1). …”

Section: Mirna Functions In C Elegansmentioning

confidence: 99%

Caenorhabditis elegans: An important tool for dissecting microRNA functions

Zhu¹,

Zhang²,

Lee³

et al. 2016

Biomed Genet Genomics

View full text Add to dashboard Cite

Caenorhabditis elegans (C. elegans), a member of the phylum Nematoda, carries the evolutionarily conserved genes comparing to mammals. Due to its short lifespan and completely sequenced genome, C. elegans becomes a potentially powerful model for mechanistic studies in human diseases. In this mini review, we will outline the current understandings on C. elegans as a model organism for microRNA (miRNA)-related research in the pathogenesis of human diseases.

show abstract

A MicroRNA-Mediated Insulin Signaling Pathway Regulates the Toxicity of Multi-Walled Carbon Nanotubes in Nematode Caenorhabditis elegans

Cited by 45 publications

References 52 publications

Identification of interneurons required for the aversive response of Caenorhabditis elegans to graphene oxide

Identification of interneurons required for the aversive response of Caenorhabditis elegans to graphene oxide

Toxicity evaluation of Wanzhou watershed of Yangtze Three Gorges Reservoir in the flood season in Caenorhabditis elegans

Caenorhabditis elegans: An important tool for dissecting microRNA functions

Contact Info

Product

Resources

About