We present a method for the computational image analysis of high
frequency guided sound waves based upon the measurement of optical
interference fringes, produced at the air interface of a thin film
of liquid. These acoustic actuations induce an affine deformation
of the liquid, creating a lensing effect that can be readily observed
using a simple imaging system. We exploit this effect to measure and
analyze the spatiotemporal behavior of the thin liquid film as the
acoustic wave interacts with it. We also show that, by investigating
the dynamics of the relaxation processes of these deformations when
actuation ceases, we are able to determine the liquid’s viscosity
using just a lens-free imaging system and a simple disposable biochip.
Contrary to all other acoustic-based techniques in rheology, our measurements
do not require monitoring of the wave parameters to obtain quantitative
values for fluid viscosities, for sample volumes as low as 200 pL.
We envisage that the proposed methods could enable high throughput,
chip-based, reagent-free rheological studies within very small samples.