The positive-displacement micropumps push out fluid into one direction by expanding and decreasing a cavity volume with various actuation mechanisms. However, the volume change of the cavity is repeated periodically to supply the fluid, which causes a periodic flow rate change, resulting in a pulse flow. Such pulsations are not suitable for use in specific applications that require stable fluid delivery, such as microsensors [12][13][14] and droplet microfluidic devices. [15,16] For example, in the application of mechanosensors that utilize bending displacements to measure the adsorption of biomolecules on cantilever surface, pulse flow can vibrate the cantilever, generating noise in the output signal. [17,18] In contrast to the positive-displacement micropumps, nonmechanical micropumps that generate pulseless flow have also been developed by using thermo-pneumatic actuation, [19,20] chemical reactions, [21] and capillary force. [22][23][24] However, there are limitations in the thermo-pneumatic actuation due to heat damage to biomaterials, while micropumps using chemical reactions or capillary force have difficulties to actively control the flow rate.As an alternative approach to supply fluids, commercially available syringe pumps are widely used in microfluidic devices. Bulky syringe pumps have disadvantages for use with microfluidic devices due to 1) the large dead volume associated with the large bore of the syringe and its connecting tubes, and 2) difficulty in inserting the whole system into an incubator. Although miniaturizing the conventional bulky syringe is in a growing demand for microfluidic devices, it is challenging due to the difficulty in fabricating and assembling complex 3D microcomponents as well as the scaling law of the mechanical sliding parts in microscale. Since the stick-slip phenomenon occurs in the micro-sized piston-cylinder, it is crucial to avoid any mechanical sliding parts to realize on-chip microsyringe pumps. Also, it is necessary to develop on-chip power sources having not only high output power density but also no mechanical sliding parts.Considering these demands, we focus on utilizing an electroconjugate fluid (ECF) for the development of on-chip microsyringe pumps. The ECF, a functional fluid, can generate an active flow (ECF jet) between a pair of electrodes when a high DC voltage is applied to the electrodes in the ECF. The ECFs are