A microgripper sensor device capable of detecting ion efflux from whole cells

Abstract

Use policyThe full-text may be used and/or reproduced, and given to third parties in any format or medium, without prior permission or charge, for personal research or study, educational, or not-for-prot purposes provided that:• a full bibliographic reference is made to the original source • a link is made to the metadata record in DRO • the full-text is not changed in any way The full-text must not be sold in any format or medium without the formal permission of the copyright holders.Please consult the full DRO policy for further details. Electrothermally actuated microgripper sensor devices that are capable of simultaneous manipulation of live mouse oocytes and the sensing of potassium ion efflux are presented. The ion selective electrode technology applied to a microgripper device yielded a first generation ion sensor with competitive characteristics in their selectivity, sensitivity, stability and temporal resolution. The microgripper sensor devices could readily detect the 9 ± 3 mM efflux of potassium ions upon mechanical stimulation. This technology is generic and applicable to generating charge sensing microgrippers.

The static and dynamic response of SU-8 electrothermal microgrippers of varying thickness

“…In other work reported previously, we have modelled and experimentally verified the heat loss mechanism in the microgripper [16], performed further heat loss studies in different ambient gases over a range of pressures [17] and demonstrated the use of the microgripper in holding a cell while simultaneously being able to detect and quantify the excretion of potassium ions using an ion specific electrode fabricated as an integral part of the structure [18].…”