A Microgranulocyte Cytotoxicity Test1

Hasegawa, Toshifumi; Graw, Robert G.; Terasaki, P. I.

doi:10.1097/00007890-197311050-00013

Cited by 5 publications

(5 citation statements)

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“…Thirty-six different granulocyte samples were tested from donors cif group A (14). B (12), AB (7), and 0 (3). Both fixed and unfixed granulocytes were tested on all occasions.…”

Section: Resultsmentioning

confidence: 99%

Granulocytes do not have surface ABO antigens

Kelton¹,

Bebenek

1985

Transfusion

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There is disagreement whether granulocytes have ABO antigens on their surfaces. We investigated this issue using pure populations of washed granulocytes from healthy individuals of known ABO, Lewis, and secretor phenotypes. The presence of ABO antigens on granulocytes was sought using four different techniques: a granulocyte-red cell mixed agglutination assay; a microleukoagglutination assay; a microgranulocyte cytotoxicity assay; and an immunoradiometric assay for granulocyte-associated IgG. Both fixed and unfixed granulocytes were tested. All tests were negative. Granulocytes do not have ABO antigens on their surfaces.

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“…Thirty-six different granulocyte samples were tested from donors cif group A (14). B (12), AB (7), and 0 (3). Both fixed and unfixed granulocytes were tested on all occasions.…”

Section: Resultsmentioning

confidence: 99%

Granulocytes do not have surface ABO antigens

Kelton¹,

Bebenek

1985

Transfusion

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“…Previous reports have emphasized the difficulty in studying granulocytes in cytotoxicity assays [2, 131. Granulocytes generally take up eosin dye nonspecifically when they adhere to the bottom of plastic wells [2]. This problem may be overcome by pretreating the cells with iodoacetamine [2], papain [I31 or alcohol [13].…”

Section: Discussionmentioning

confidence: 99%

An antigen expressed by cells of the myelo‐monocytic lineage

Foon

Billing²,

Fitchen

et al. 1981

American J Hematol

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We describe an antigen(s) characterized by a heteroantiserum raised in rabbits against mature human granulocytes. This antigen was found on neutrophils, monocytes, platelets, acute and chronic myelocytic leukemia cells and on granulocyte-macrophage progenitor cells grown in agar. It was not found on lymphocytes, eosinophils, erythrocytes, or erythroid progenitor cells. On the basis of tissue distribution and absorption studies, the antigen (tentatively designated the "myelo-monocytic" antigen) is distinct from antigens previously identified on human neutrophils. Restriction of the "myelo-monocytic" antigen to normal and malignant cells of the myelo-monocytic series suggests that it may represent a normal differentiation antigen of the myelo-monocytic lineage.

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“…Antibodies to NA1 and NA2 were also examined with NA1‐ and NA2‐typed donor granulocytes, respectively. The patient's sera sample after BMT and the supernatant of the donor's marrow were also examined for the presence of anti‐HLA Class I antibody by a lymphocyte cytotoxic test 9 …”

Section: Methodsmentioning

confidence: 99%