A microfluorimetric study of translational diffusion in erythrocyte membranes

Peters, Reiner; Peters, Jutta; Tews, Karl Heinz; Bähr, Wolfgang

doi:10.1016/0005-2736(74)90085-6

Cited by 436 publications

(207 citation statements)

References 25 publications

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“…Mathematical descriptions of lateral diffusion have already been given by others [6,17] for various membrane models. The sophisticated solutions which have been proposed for planar systems [6] cannot be used without powerful computers.…”

Section: Translational Diffusion Of 12-(9-anthroyl)straric Acid In Momentioning

confidence: 99%

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A Fluorescence Approach of the Determination of Translational Diffusion Coefficients of Lipids in Phospholipid Monolayer at the Air-Water Interface

Teissié¹,

Tocanne²,

Baudras³

1978

Eur J Biochem

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In the present work, it is shown that the photobleaching technique as well as experimentation based on fluorescence recovery after bleaching can be extended to monolayers spread at the air-water interface. A mathematical model is derived which allows the determination of translational diffusion coefficients of species diffusing in such a system. Using 12-(9-anthroyl)stearic acid (anthroylstearate) as a fluorescent probe, dispersed either in dipalmitoylphosphatidylcholine or in dipalmitoylphosphatidylglycerol in various conditions of subphase ionic composition and surface pressure of the monolayer, including phase transition domains, we are led to the following conclusions :1. Anthroylstearate molecules seem to aggregate in 'microdomains' where their fluorescence properties remain unchanged regardless of the compression states of the host monolayer.2. In any case, a break in the diffusion constants appears on compressing films of both dipalmitoylphosphatidylcholine and dipalmitoylphosphatidylglycerol. In particular, this break coincides with the liquid expanded to gel phase transition of these lipids when it occurs.3. Diffusion of anthroylstearate in dipalmitoylphosphatidylglycerol depends strongly on the subphase ionic strength and on the nature of cations: Na', Mg2+, Ca2+.In recent years, much interest has been expressed in the study of lateral diffusion of phospholipids and proteins in biomembranes. Such a process which participates in the more general concept of 'membrane fluidity' seems to play a significant role in modulating membrane functions of some proteins [l]. Many of the studies reported on lateral diffusion have been carried out by use of spectroscopic methods, basically electron spin resonance [2], nuclear magnetic resonance , whereas experimentation on monolayers have been scarce and based on the use of radioactive tracers [lo]. Lipid monolayers at the air-water interface have proved to be good membrane models in which not only the temperature and subphase pH and ionic composition can be controlled, but also the molecular packing of the lipids. We have reported recently [ 111 that fluorescence measurements are feasible with such monolayers at very low concentrations of the probe (mole ratio of the order of In the present work, the photobleaching technique has been extended to monolayers and the experimentation based on fluorescence recovery after bleaching as well. A mathematical treatment of the data has been derived which allows the determination of translational diffusion coefficients in such a system. The results presented deal with the diffusion of 12-(9-anthroy1)stearic acid (anthroylstearate) dispersed either in dipalmitoylphosphatidylcholine or in dipalmitoylphosphatidylglycerol in various conditions of subphase ionic composition and surface pressure of the monolayer, including phase transition domains. 1 : 100).

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Section: Translational Diffusion Of 12-(9-anthroyl)straric Acid In Momentioning

confidence: 99%

“…The right term of Eqn (17) was computed using un from tables [20] and for different D and I values. Then, diffusion coefficients D were obtained through a graphical resolution of Eqn (17).…”

Section: Translational Diffusion Of 12-(9-anthroyl)straric Acid In Momentioning

confidence: 99%

A Fluorescence Approach of the Determination of Translational Diffusion Coefficients of Lipids in Phospholipid Monolayer at the Air-Water Interface

Teissié¹,

Tocanne²,

Baudras³

1978

Eur J Biochem

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“…198 1976); a minor immobilized fraction becomes free when the 2.1 is extracted. Nevertheless the lateral diffusion of band 3 is very slow as long as the cytoskeleton remains in place (Peters et al, 1974;Fowler & Branton, 1977). These observations can most simply be reconciled if one supposes the band 3, projecting inwards into the cytoplasm, to be physically confined within the interstices of the cytoskeletal net, its rate of diffusion being limited by the stability of this structure.…”

mentioning

confidence: 99%

The red cell membrane and its cytoskeleton

Gratzer¹

1981

Biochemical Journal

126

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“…14,15 The first FRAP studies were designed to measure the lateral mobility of membrane proteins and test the fluid mosaic model of membrane structure. 16,17 The theory for analyzing these experiments accounted only for diffusion. The FRAP experimental design was later adapted to three dimensional molecule mobility and laser scanning confocal microscopy.…”

Section: Binding Rate Constants Determined In Cellsmentioning

confidence: 99%

The biochemistry of RNA metabolism studied in situ

Nickerson

2009

RNA Biology

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A microfluorimetric study of translational diffusion in erythrocyte membranes

Cited by 436 publications

References 25 publications

A Fluorescence Approach of the Determination of Translational Diffusion Coefficients of Lipids in Phospholipid Monolayer at the Air-Water Interface

A Fluorescence Approach of the Determination of Translational Diffusion Coefficients of Lipids in Phospholipid Monolayer at the Air-Water Interface

The red cell membrane and its cytoskeleton

The biochemistry of RNA metabolism studied in situ

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