A microfluidic tool for real-time impedance monitoring of in vitro renal tubular epithelial cell barrier

Feng, Liang; Huang, Xiaochen; Huang, Boxin; He, Yuqing; Luo, Haoyue; Shi, Jian; Wang, Li; Aimé, Carole; Peng, Juan; Chen, Yong

doi:10.1016/j.snb.2023.134077

Cited by 4 publications

(4 citation statements)

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“…The magnitude and phase values are determined for each frequency to obtain the impedance spectrum of the tissue sample, commonly referred to as electrical impedance spectroscopy (EIS). Due to its versatility of revealing impedance information across a wide range of frequencies, EIS is a widely-used technique to discover the impedance characteristics of tissue/cell-culture samples in Ussing Chambers, Organ-on-a-chip devices, and well inserts 8,12,19,20,23,[26][27][28][29][30][31] .…”

Section: Teer Calculationmentioning

confidence: 99%

“…To quantify the integrity of tight junctions in an epithelial barrier, many researchers have performed experiments to determine the TEER of the barrier. Junction integrity of epithelial barriers can also be evaluated by using fluorescently labelled probes to visualize the paracellular flux across the epithelium [9][10][11][12][13][14] . Most of the studies using TEER to measure the integrity of tight junctions in epithelial barriers use in-vitro cell culture models of endothelial and epithelial monolayers in a Transwell system 12,[15][16][17] .…”

Section: Introductionmentioning

confidence: 99%

“…Junction integrity of epithelial barriers can also be evaluated by using fluorescently labelled probes to visualize the paracellular flux across the epithelium [9][10][11][12][13][14] . Most of the studies using TEER to measure the integrity of tight junctions in epithelial barriers use in-vitro cell culture models of endothelial and epithelial monolayers in a Transwell system 12,[15][16][17] . More recently, organ-on-a-chip (OoC) devices have been proposed.…”

Section: Introductionmentioning

confidence: 99%

“…More recently, organ-on-a-chip (OoC) devices have been proposed. These are more advanced devices which often incorporate microfluidics for maintaining cell culture 12,[18][19][20] . These devices are made to resemble in vivo environments more accurately, both biochemically and physically 10,21 .…”

Section: Introductionmentioning

confidence: 99%

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A Microphysiological System for Studying Barrier Health of Live Tissues in Real Time

Chen,

Way,

Templeton

et al. 2024

Preprint

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Epithelial cells create barriers that protect many different components in the body from their external environment. The gut in particular carries bacteria and other infectious agents. A healthy gut epithelial barrier prevents unwanted substances from accessing the underlying lamina propria while maintaining the ability to digest and absorb nutrients. Increased gut barrier permeability, better known as leaky gut, has been linked to several chronic inflammatory diseases. Yet understanding the cause of leaky gut and developing effective interventions are still elusive due to the lack of tools to maintain tissue’s physiological environment while elucidating cellular functions under various stimuli ex vivo. This paper presents a microphysiological system capable of recording real-time barrier permeability of mouse gut tissues in a realistic physiological environment over extended durations. Key components of the microphysiological system include a microfluidic chamber designed to hold the live tissue explant and create a sufficient microphysiological environment to maintain tissue viability; proper media composition that preserves a microbiome and creates necessary oxygen gradients across the barrier; integrated sensor electrodes and supporting electronics for acquiring and calculating transepithelial electrical resistance (TEER); and a scalable system architecture to allow multiple chambers running in parallel for increased throughput. The experimental results demonstrate that the system can maintain tissue viability for up to 72 hours. The results also show that the custom-built and integrated TEER sensors are sufficiently sensitive to distinguish differing levels of barrier permeability when treated with collagenase and low pH media compared to control. Permeability variations in tissue explants from different positions in the intestinal tract were also investigated using TEER revealing their disparities in permeability. Finally, the results also quantitatively determine the effect of the muscle layer on total epithelial resistance.

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