A microfluidic system combining acoustic and dielectrophoretic particle preconcentration and focusing

Ravula, Surendra K.; Branch, Darren W.; James, Conrad D.; Townsend, R.J.; Hill, Martyn; Kaduchak, Gregory; Ward, MA; Brener, Igal

doi:10.1016/j.snb.2007.10.024

Cited by 45 publications

(28 citation statements)

References 21 publications

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“…By coupling acoustic waves into an interdigitated microelectrode system, particles can first be preconcentrated before focusing them into flow channels and to precise locations using DEP forces. 78 Rajaraman et al 79 describe rapid and low cost microfabrication methods to produce electrode arrays of the interdigitated, castellated geometry.…”

Section: Technologymentioning

confidence: 99%

Review Article—Dielectrophoresis: Status of the theory, technology, and applications

2010

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A review is presented of the present status of the theory, the developed technology and the current applications of dielectrophoresis ͑DEP͒. Over the past 10 years around 2000 publications have addressed these three aspects, and current trends suggest that the theory and technology have matured sufficiently for most effort to now be directed towards applying DEP to unmet needs in such areas as biosensors, cell therapeutics, drug discovery, medical diagnostics, microfluidics, nanoassembly, and particle filtration. The dipole approximation to describe the DEP force acting on a particle subjected to a nonuniform electric field has evolved to include multipole contributions, the perturbing effects arising from interactions with other cells and boundary surfaces, and the influence of electrical double-layer polarizations that must be considered for nanoparticles. Theoretical modelling of the electric field gradients generated by different electrode designs has also reached an advanced state. Advances in the technology include the development of sophisticated electrode designs, along with the introduction of new materials ͑e.g., silicone polymers, dry film resist͒ and methods for fabricating the electrodes and microfluidics of DEP devices ͑photo and electron beam lithography, laser ablation, thin film techniques, CMOS technology͒. Around three-quarters of the 300 or so scientific publications now being published each year on DEP are directed towards practical applications, and this is matched with an increasing number of patent applications. A summary of the US patents granted since January 2005 is given, along with an outline of the small number of perceived industrial applications ͑e.g., mineral separation, micropolishing, manipulation and dispensing of fluid droplets, manipulation and assembly of micro components͒. The technology has also advanced sufficiently for DEP to be used as a tool to manipulate nanoparticles ͑e.g., carbon nanotubes, nano wires, gold and metal oxide nanoparticles͒ for the fabrication of devices and sensors. Most efforts are now being directed towards biomedical applications, such as the spatial manipulation and selective separation/enrichment of target cells or bacteria, high-throughput molecular screening, biosensors, immunoassays, and the artificial engineering of three-dimensional cell constructs. DEP is able to manipulate and sort cells without the need for biochemical labels or other bioengineered tags, and without contact to any surfaces. This opens up potentially important applications of DEP as a tool to address an unmet need in stem cell research and therapy.

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Section: Technologymentioning

confidence: 99%

Review Article—Dielectrophoresis: Status of the theory, technology, and applications

2010

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“…Therefore, the acceleration term can be safely neglected, and it can be assumed that the particles move with the terminal speed at all times. Substituting Eqs (19) and (20) into Eq. (26), and introducing the aforementioned scale factor [9,26,37,38], the particle velocity can be obtained as…”

Section: Simulation Of the Particle Trajectorymentioning

confidence: 99%

“…However, in the case of direct current field, the non-conducting particles and cells experience only n-DEP [9]. DEP has been successfully implemented for cell sorting [10,11], sample preparation [12,13], cell detection [14,15,16], cell trapping [17], particle focusing [18,19], cell characterization [20] and cell patterning [21,22]. The dependence of the DEP force on the size and the electrical properties makes it possible for cell/ particle separation based on the size and electrical properties differences.…”

Section: Introductionmentioning

confidence: 99%

Continuous particle separation based on electrical properties using alternating current dielectrophoresis

Çetin

2009

Electrophoresis

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A design of a microchannel for continuous separation of particles based on alternating current dielectrophoretic is studied. The geometric design parameters are determined by analyzing the dielectrophoresis force field inside the microchannel corresponding to the most efficient separation. The trajectories of 5 and 10 mm spherical particles are derived analytically using Lagrangian tracking method to examine the feasibility and the effectiveness of the design. The effects of the mean flow velocity inside the channel and the applied voltage across the channel on the performance of the separation are also discussed.

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“…In addition to the possibility of sorting cells through balancing of forces the longer range acoustic forces were used to pre-align populations of cells in preparation for more locally specific DEP switching/manipulation operations. Ravula et al 45 describe a layout that utilised the levitation forces generated by ultrasound in a planar resonator coupled to either glass or silicon-substrate DEP chips. Particles are preconcentrated and aligned by the acoustic radiation forces to increase throughput and reduce the positional variability of particles prior to fine adjustments by the DEP electrodes.…”

Section: Forces Induced By Electrical Fieldsmentioning

confidence: 99%

Acoustofluidics 23: acoustic manipulation combined with other force fields

Glynne‐Jones

Hill

2013

Lab Chip

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In this, the final paper of the Acoustofluidics series of tutorial articles, we discuss applications in which acoustic radiation forces are used in conjunction with competing or complementary forcefields. This may be in order to enable manipulation operations that would not be easily performed by either force-field alone, or may be used to effect separation based on the different physical principals underlying competing fields. Examples are given of a number of different applications in which acoustic forces are combined with gravitational fields, hydrodynamic forces, electric fields (including dielectrophoresis), magnetic forces and optical forces.

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A microfluidic system combining acoustic and dielectrophoretic particle preconcentration and focusing

Cited by 45 publications

References 21 publications

Review Article—Dielectrophoresis: Status of the theory, technology, and applications

Review Article—Dielectrophoresis: Status of the theory, technology, and applications

Continuous particle separation based on electrical properties using alternating current dielectrophoresis

Acoustofluidics 23: acoustic manipulation combined with other force fields

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