A Microfluidic Stretch System Upregulates Resistance Exercise-Related Pathway

Ahn, Jisong; Kim, Jaesang; Jeon, Jessie S.; Jang, Young Jin

doi:10.1007/s13206-022-00051-6

Cited by 5 publications

(2 citation statements)

References 40 publications

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“…After that, the protein was transferred to the nitrocellulose membrane via sodium dodecyl-polyacrylamide gel electrophoresis (SDS-PAGE), and the membrane was incubated with 1x TBST containing 0.1% Tween-20 and blocked with skimmed milk at room temperature for 1 h. The blocked membrane was incubated diluted in PARP, p53, p21, caspase 3, Puma, Bax, Cleaved Caspase 3, RPL5, p-JNK, JNK, p-ERK, ERK, p-p38, p38 or γH2AX (1:1000) primary antibodies, or β-actin (1:5000) at 4 °C, then incubated overnight. After that, the secondary antibodies, IgG-HRP, and IgG-HRP, were cultured at room temperature for 1 h, and Image Quant LAS 500 (GE Healthcare Life Sciences, Sydney, NSW, Australia) was used for measurement [ 48 ].…”

Section: Methodsmentioning

confidence: 99%

Antitumor Effect of Cycloastragenol in Colon Cancer Cells via p53 Activation

Park

Jung

et al. 2022

IJMS

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Colorectal cancer cell (CRC) is the fourth most common cancer in the world. There are several chemotherapy drugs available for its treatment, though they have side effects. Cycloastragenol (CY) is a compound from Astragalus membranaceus (Fisch.) Bge known to be effective in aging, anti-inflammatory, anticancer, and anti-heart failure treatments. Although many studies have demonstrated the functions of CY in cancer cells, no studies have shown the effects of p53 in colon cancer cells. In this study, we found that CY reduces the viability of colon cancer cells in p53 wild-type cells compared to p53 null cells and HT29. Furthermore, CY induces apoptosis by p53 activation in a dose- and time-dependent manner. And it was confirmed that it affects the L5 gene related to p53. Additionally, CY enhanced p53 expression compared to when either doxorubicin or 5-FU was used alone. Altogether, our findings suggest that CY induces apoptosis via p53 activation and inhibits the proliferation of colon cancer cells. In addition, apoptosis occurs in colon cancer cells due to other factors. Moreover, CY is expected to have a combined effect when used together with existing treatments for colon cancer in the future.

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Section: Methodsmentioning

confidence: 99%

Antitumor Effect of Cycloastragenol in Colon Cancer Cells via p53 Activation

Park

Jung

et al. 2022

IJMS

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“…Organ-specific movements, such as peristalsis, periodic stretching and contractions, and shear stress by fluid, can influence bacterial adhesion and growth as well as regulate host cell behaviors that involve defense mechanisms. Various microphysiological systems with dynamic stimuli and movements can be particularly useful for generating dynamic bacterial coculture platforms [ 118 – 120 ].…”

Section: Challenges and Opportunities In Organotypic Bacterial Cocult...mentioning

confidence: 99%

Advanced Organotypic In Vitro Model Systems for Host–Microbial Coculture

2023

BioChip J

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In vitro model systems have been advanced to recapitulate important physiological features of the target organ in vivo more closely than the conventional cell line cultures on a petri dish. The advanced organotypic model systems can be used as a complementary or alternative tool for various testing and screening. Numerous data from germ-free animal studies and genome sequencings of clinical samples indicate that human microbiota is an essential part of the human body, but current in vitro model systems rarely include them, which can be one of the reasons for the discrepancy in the tissue phenotypes and outcome of therapeutic intervention between in vivo and in vitro tissues. A coculture model system with appropriate microbes and host cells may have great potential to bridge the gap between the in vitro model and the in vivo counterpart. However, successfully integrating two species in one system introduces new variables to consider and poses new challenges to overcome. This review aims to provide perspectives on the important factors that should be considered for developing organotypic bacterial coculture models. Recent advances in various organotypic bacterial coculture models are highlighted. Finally, challenges and opportunities in developing organotypic microbial coculture models are also discussed.

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