A microfluidic platform forin situstudies of bacteria electroporation
Ivan L. Volkov,
Zahra Khaji,
Magnus Johansson
et al.
Abstract:Electroporation of dye-labelled bio-molecules has proven to be a valuable alternative to fluorescent protein fusion for single-molecule tracking in living cells. However, control over cell viability, electroporation efficiency and environment conditions before, during and after electroporation is difficult to achieve in bulk experiments. Here, we present a microfluidic platform capable of single-cell electroporation with in situ microscopy and demonstrate delivery of DNA into bacteria. Via real time observatio… Show more
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