A microfluidic platform enables comprehensive gene expression profiling of mouse retinal stem cells

Coles, Brenda L.K.; Labib, Mahmoud; Poudineh, Mahla; Innes, Brendan T.; Belair-Hickey, Justin; Gomis, Surath; Wang, Zongjie; Bader, Gary D.; Sargent, Edward H.; Kooy, Derek van der

doi:10.1039/d1lc00790d

Cited by 4 publications

(4 citation statements)

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“…The study of stem cell deformation using microfluidic technology is of significant importance to regenerative medicine and tissue engineering. By understanding stem cell responses to mechanical forces and deformation, researchers can better control their differentiation fate and increase their therapeutic potential ( Coles et al, 2021 ). Furthermore, microfluidic technology can be utilized to screen drugs and growth factors that can regulate stem cell deformation and differentiation, ultimately leading to the development of novel stem cell-based therapies for various diseases and injuries ( Hodgson et al, 2017 ), ( Sibbitts et al, 2018 ).…”

Section: Applications In Clinical Diagnosismentioning

confidence: 99%

Measuring cell deformation by microfluidics

Jiang

Zhao

et al. 2023

Front. Bioeng. Biotechnol.

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Microfluidics is an increasingly popular method for studying cell deformation, with various applications in fields such as cell biology, biophysics, and medical research. Characterizing cell deformation offers insights into fundamental cell processes, such as migration, division, and signaling. This review summarizes recent advances in microfluidic techniques for measuring cellular deformation, including the different types of microfluidic devices and methods used to induce cell deformation. Recent applications of microfluidics-based approaches for studying cell deformation are highlighted. Compared to traditional methods, microfluidic chips can control the direction and velocity of cell flow by establishing microfluidic channels and microcolumn arrays, enabling the measurement of cell shape changes. Overall, microfluidics-based approaches provide a powerful platform for studying cell deformation. It is expected that future developments will lead to more intelligent and diverse microfluidic chips, further promoting the application of microfluidics-based methods in biomedical research, providing more effective tools for disease diagnosis, drug screening, and treatment.

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Section: Applications In Clinical Diagnosismentioning

confidence: 99%

Measuring cell deformation by microfluidics

Jiang

Zhao

et al. 2023

Front. Bioeng. Biotechnol.

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“…[9][10] Current singlecell isolation and analysis approaches involve fluorescence-activated cell sorting (FACS), [11][12][13] magnetic activated cell sorting (MACS), [8,14] laser capture microdissection (LCM), [15][16][17] and many microfluidic techniques. [18][19][20][21] Microfluidic methods offer many benefits including portability, low cost, microliter-scale sample consumption, ability to observe and image the cells, and amenability to multistep or multi-endpoint analyses.…”

Section: Introductionmentioning

confidence: 99%

Dielectrophoretic Capture and Electrochemical Enzyme‐Linked Immunosorbent Assay of Single Melanoma Cells at an Array of Interlocked Spiral Bipolar Electrodes

Clark,

Moser,

Anand

2024

ChemElectroChem

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Analysis of single cancer cells is critical to obtain accurate patient diagnosis and prognosis. In this work, we report the selective dielectrophoretic capture and electrochemical analysis of single melanoma cells at an array of interlocked spiral bipolar electrodes (iBPEs). Following dielectrophoretic capture, individual melanoma cells are hydrodynamically transferred into picoliter‐scale chambers for subsequent analysis. The interlocked spiral end of the iBPE (the sensing pole) is utilized to read out an electrochemical enzyme‐linked immunosorbent assay (eELISA), which quantifies the expression of a cell surface antigen, melanoma cell adhesion marker (MCAM). The opposite pole of each BPE is located in a fluidically isolated compartment containing reagents for electrogenerated chemiluminescence (ECL), such that luminescence reports iBPE current. In a preliminary device design, the ECL intensity was insufficient to detect MCAM expression on single cells. To achieve single‐cell analysis, we decreased the gap size between the interlocked spirals tenfold (5.0 μm to 0.5 μm), thereby creating a more sensitive biosensor by enhanced redox cycling of the product of eELISA. This work is significant because it allows for the selective isolation and sensitive analysis of individual melanoma cells in a device amenable to point‐of‐care (POC) application by combining dielectrophoresis (DEP) with interdigitated bipolar electrodes (IDBPEs).

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“…Somatic or adult stem cells (ASCs) do not pose ethical concerns as PSCs. Examples of ASCs include mesenchymal stem cells (MSCs) in bone marrow and adipose tissues [22], hematopoietic stem cells (HSCs) from umbilical cord blood [23], neural stem cells in nervous systems [24], epithelial stem cells in intestinal crypt [25], and retinal stem cells in retinal periphery [26], and skin stem cells located in the epidermis [27]. The origin of ASCs makes their isolation straightforward.…”

Section: Introductionmentioning

confidence: 99%

Assessing Tumorigenicity in Stem Cell-Derived Therapeutic Products: A Critical Step in Safeguarding Regenerative Medicine

Wang

2023

Bioengineering

Self Cite

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Stem cells hold promise in regenerative medicine due to their ability to proliferate and differentiate into various cell types. However, their self-renewal and multipotency also raise concerns about their tumorigenicity during and post-therapy. Indeed, multiple studies have reported the presence of stem cell-derived tumors in animal models and clinical administrations. Therefore, the assessment of tumorigenicity is crucial in evaluating the safety of stem cell-derived therapeutic products. Ideally, the assessment needs to be performed rapidly, sensitively, cost-effectively, and scalable. This article reviews various approaches for assessing tumorigenicity, including animal models, soft agar culture, PCR, flow cytometry, and microfluidics. Each method has its advantages and limitations. The selection of the assay depends on the specific needs of the study and the stage of development of the stem cell-derived therapeutic product. Combining multiple assays may provide a more comprehensive evaluation of tumorigenicity. Future developments should focus on the optimization and standardization of microfluidics-based methods, as well as the integration of multiple assays into a single platform for efficient and comprehensive evaluation of tumorigenicity.

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A microfluidic platform enables comprehensive gene expression profiling of mouse retinal stem cells

Abstract: Loss of photoreceptors due to retinal degeneration is a major cause of untreatable visual impairment and blindness. Cell replacement therapy, using retinal stem cell (RSC)-derived photoreceptors, holds promise for reconstituting...

Cited by 4 publications

References 49 publications

Measuring cell deformation by microfluidics

Measuring cell deformation by microfluidics

Dielectrophoretic Capture and Electrochemical Enzyme‐Linked Immunosorbent Assay of Single Melanoma Cells at an Array of Interlocked Spiral Bipolar Electrodes

Assessing Tumorigenicity in Stem Cell-Derived Therapeutic Products: A Critical Step in Safeguarding Regenerative Medicine

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