A microfluidic paper-based laser-induced fluorescence sensor based on duplex-specific nuclease amplification for selective and sensitive detection of miRNAs in cancer cells

Cai, Xiaoyu; Zhang, Huimin; Yu, Xiufeng; Wang, Wei

doi:10.1016/j.talanta.2020.120996

Cited by 42 publications

(21 citation statements)

References 34 publications

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“…Many studies have confirmed that the detection performance of microfluidic lab-on-paper platforms can be improved through the use of sample pre-concentration techniques (such as ICP, ITP, EKS, and so on) [72,73,75,115,171], or amplification devices (e.g., LAMP, PCR, CHR, HCR, and so forth.) [78,81,83,172,173]. For example, the authors in [84] showed that the detection performance of a lab-on-paper platform for urine microalbuminuria diagnosis in diabetic patients could be significantly improved by incorporating EKS with a 100-fold amplification capability in the sample pre-treatment stage.…”

Section: Discussionmentioning

confidence: 99%

Lab-on-Paper Devices for Diagnosis of Human Diseases Using Urine Samples—A Review

et al. 2021

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In recent years, microfluidic lab-on-paper devices have emerged as a rapid and low-cost alternative to traditional laboratory tests. Additionally, they were widely considered as a promising solution for point-of-care testing (POCT) at home or regions that lack medical infrastructure and resources. This review describes important advances in microfluidic lab-on-paper diagnostics for human health monitoring and disease diagnosis over the past five years. The review commenced by explaining the choice of paper, fabrication methods, and detection techniques to realize microfluidic lab-on-paper devices. Then, the sample pretreatment procedure used to improve the detection performance of lab-on-paper devices was introduced. Furthermore, an in-depth review of lab-on-paper devices for disease measurement based on an analysis of urine samples was presented. The review concludes with the potential challenges that the future development of commercial microfluidic lab-on-paper platforms for human disease detection would face.

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Section: Discussionmentioning

confidence: 99%

Lab-on-Paper Devices for Diagnosis of Human Diseases Using Urine Samples—A Review

et al. 2021

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“…It is mainly used to determine the fluorescence samples and fluorescent markers by using the fluorescence of some substances emitted by light irradiation. The laser induced fluorescence (LIF) detection system 96 is matched with the size of the chip. Due to the low scattering of the laser beam, ideal results can be obtained with only a small volume of sample.…”

Section: Paper Chip Detection Methodsmentioning

confidence: 99%

Research progress on the applications of paper chips

Tong

Zhao

et al. 2021

RSC Adv.

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“…The paper-based sensor enabled genomic DNA's identification for Escherichia coli and Campylobacter jejuni, with the LOD of 2 × 10 3 copies/μL. Other μPads for nucleic acid detection [188][189][190][191][192] have been summarized in Table 2. Another important application for μPads is to directly detect cells.…”

Section: Biochemical Analysismentioning

confidence: 99%

Recent Advances and Applications in Paper-Based Devices for Point-of-Care Testing

Hou

Guo

et al. 2022

J. Anal. Test.

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Point-of-care testing (POCT), as a portable and user-friendly technology, can obtain accurate test results immediately at the sampling point. Nowadays, microfluidic paper-based analysis devices (μPads) have attracted the eye of the public and accelerated the development of POCT. A variety of detection methods are combined with μPads to realize precise, rapid and sensitive POCT. This article mainly introduced the development of electrochemistry and optical detection methods on μPads for POCT and their applications on disease analysis, environmental monitoring and food control in the past 5 years. Finally, the challenges and future development prospects of μPads for POCT were discussed.

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A microfluidic paper-based laser-induced fluorescence sensor based on duplex-specific nuclease amplification for selective and sensitive detection of miRNAs in cancer cells

Cited by 42 publications

References 34 publications

Lab-on-Paper Devices for Diagnosis of Human Diseases Using Urine Samples—A Review

Lab-on-Paper Devices for Diagnosis of Human Diseases Using Urine Samples—A Review

Research progress on the applications of paper chips

Recent Advances and Applications in Paper-Based Devices for Point-of-Care Testing

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