A microfluidic finger-actuated blood lysate preparation device enabled by rapid acoustofluidic mixing

Haque, Md Ehtashamul; Conde, Alvaro J.; MacPherson, William N.; Knight, Stephen R; Carter, Richard M.; Kersaudy-Kerhoas, Maïwenn

doi:10.1039/d2lc00968d

Cited by 8 publications

(2 citation statements)

References 47 publications

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“…18,19 Traditional microfluidic chips often require external pumps and valves to provide a power source, but the dependence on electrical equipment is not good for on-site detection. Therefore, some power-free microfluidic chips, such as centrifugal chips, 20 capillary forcedriven chips 21 and finger-driven chips 22 have been explored. Among them, finger-driven chips were often reported using finger-actuated pumps with a sliding valve, 23 pneumatic valve, 24 and magnetorheological elastomer valve.…”

Section: Introductionmentioning

confidence: 99%

Multiplex nanozymatic biosensing of Salmonella on a finger-actuated microfluidic chip

Jin,

Jiang,

Yang

et al. 2024

Lab Chip

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Section: Introductionmentioning

confidence: 99%

Multiplex nanozymatic biosensing of Salmonella on a finger-actuated microfluidic chip

Jin,

Jiang,

Yang

et al. 2024

Lab Chip

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“…Of course, these systems rely on CD-like chips and bulky equipment for the administration of controlled centrifugal forces. Finally, it is worth mentioning that, as far as valving is included, any reduction of external hardware becomes an extra task for the user, such as in the case of the finger-actuated devices that were reported for metering and dispensing [ 26 , 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Valveless On-Chip Aliquoting for Molecular Diagnosis

Romero Deza,

Schaumburg,

Berli

2023

Micromachines

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The detection of nucleic acids as specific markers of infectious diseases is commonly implemented in molecular biology laboratories. The translation of these benchtop assays to a lab-on-a-chip format demands huge efforts of integration and automation. The present work is motivated by a strong requirement often posed by molecular assays that combine isothermal amplification and CRISPR/Cas-based detection: after amplification, a 2–8 microliter aliquot of the reaction products must be taken for the subsequent reaction. In order to fulfill this technical problem, we have designed and prototyped a microfluidic device that is able to meter and aliquot in the required range during the stepped assay. The operation is achieved by integrating a porous material that retains the desired amount of liquid after removing the excess reaction products, an innovative solution that avoids valving and external actuation. The prototypes were calibrated and experimentally tested to demonstrate the overall performance (general fluidics, metering, aliquoting, mixing and reaction). The proposed aliquoting method is fully compatible with additional functions, such as sample concentration or reagent storage, and could be further employed in alternative applications beyond molecular diagnosis.

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Machine learning and metaheuristics in microfluidic transport characterization and optimization: CFD and experimental study integrated with predictive modelling

Kouhkord,

Amirmahani,

Hassani

et al. 2024

Can J Chem Eng

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This study presents a comprehensive numerical and experimental analysis on microfluidic cell lysis through computational fluid dynamics (CFD), data‐driven modelling, and multi‐objective optimization. The proposed intelligent framework integrates artificial intelligence and CFD for data generation and extraction, alongside machine learning analysis and experimental studies for transport phenomena characterization in the cell lysis process. The framework explores compound effects of various inflow Reynolds numbers and geometrical parameters, including obstacle configurations and microchannel thickness. It shows substantial effects on flow patterns and mixing in varied microfluidic designs. A surrogate model, developed via central composite design, exhibits high accuracy in assessing system functionality (). The height of the implemented baffles from its lower value to the upper bound resulted in more than 42% and 14% increase in the mixing index at low and high Reynolds numbers, respectively, with minimal impact on pressure drop. The framework introduces data‐driven modelling coupled with multi‐objective optimization by desirability function (DF), non‐dominated sorting genetic algorithm (NSGA‐II), and differential evolution (DE). In the optimization of microfluidic processes, machine learning algorithms outperform desirability‐based methods, and the DE algorithm surpasses the NSGA‐II. An optimum micromixing reducing the mixing length by over 50% and mixing index above 97% achieved, fabricated, and experimental investigations conducted to validate numerical process. Through the precise control of microfluidic variables and the exploitation of microtransfer phenomena, it is possible to enhance the efficiency and selectivity of cell lysis. This not only improves the accuracy of diagnostic information but also opens up new avenues for personalized medicine and therapeutic development.

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A microfluidic finger-actuated blood lysate preparation device enabled by rapid acoustofluidic mixing

Abstract: In many blood-based diagnostic tests, including prophylactic drug analysis and malaria assays, erythrocytes, or red blood cells, must be lysed effectively prior to their use in an analytical workflow. We...

Cited by 8 publications

References 47 publications

Multiplex nanozymatic biosensing of Salmonella on a finger-actuated microfluidic chip

Multiplex nanozymatic biosensing of Salmonella on a finger-actuated microfluidic chip

Valveless On-Chip Aliquoting for Molecular Diagnosis

Machine learning and metaheuristics in microfluidic transport characterization and optimization: CFD and experimental study integrated with predictive modelling

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