A microfluidic chip for the determination of polyphenolic antioxidants

Abstract

A microfluidic system of capillary electrophoresis with electrochemical detection is developed. The process of manufacturing a chip based on polydimethylsiloxane and glass for the determination of elec troactive compounds is described. We studied the possibilities of the treatment of the chip channel surface in a gas discharge with sodium dodecyl sulfate, sodium deoxycholate, and an ionic liquid of 1 dodecyl 3 trim ethylimidazolium chloride, used as additives to the background electrolyte. It was found that the developed microfluidic system can be used for the determination of polyphenolic antioxidants in green tea and red wine.

Application of Capillary Electrophoresis to the Analysis of Bioactive Compounds in Herbal Raw Materials

“…In recent years, more and more attention has been paid to new, more improved solutions with the use of lab-on-a-chip microfluidic devices [ 65 ]. They enable fast and highly sensitive analysis and improvement of the repeatability of analyzes.…”

“…Microfluidic analytical system (MFAS) was used for determination of epigallocatechin gallate (EGCG), epicatechin (EC), epicatechin gallate (ECG), and epigallocatechin (EGC) in green tea [ 65 ]. In this paper, authors manufacturing a chip based on polydimethylsiloxane and glass.…”

“…Under optimum conditions (20 mM borate buffer electrolyte, pH 9.2) LOD for EC, EGC, ECG, and EGCG was 3.5, 3.5, 3.2, and 2.3 µg mL −1 . The concentration of EGCG, GCG, EC, ECG, and EGC was 167 ± 15, 44 ± 8, 162 ± 15, 67± 9, and 4 ± 2, respectively [ 65 ]. Application of conductively detector is very sensitive and suitable.…”