A Microfluidic Chip for LAMP-based Multiplex Detection of Pathogen

Abstract

Early diagnosis of bacterial causing the disease is important for treatment of patent and preventing the spread of pathogen. Utilizing of POCT devices to detect the pathogens on-site will accelerate the diagnosis of infectious disease. By using loop-mediated-amplification, we developed a microfluidic chip for multiplex detection of three bacterial, where the samples were driven by negative pressure were loaded quickly. The performance of the device was preliminarily evaluated. The specificities of the detections were demonstrated. And the LOD for Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa were measured as 17.15, 5.67 and 16.47 ng/μL, respectively. The results demonstrated the feasibility of the method.

Point‐of‐Care Diagnostic Platforms for Loop‐Mediated Isothermal Amplification

“…[3] The emerging isothermal amplification techniques, especially loop-mediated isothermal amplification (LAMP), overcome the limitations of conventional PCR. [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23] LAMP is a protocol for nucleic acid amplification that was first introduced in 2000. [24] It is not affected by inhibitors and operates at the same temperature throughout the assay run, eliminating the need for thermal cycling and high-cost equipment.…”