A Microfluidic Chip Architecture Enabling a Hypoxic Microenvironment and Nitric Oxide Delivery in Cell Culture

Barmaki, Samineh; Obermaier, Daniela; Kankuri, Esko; Vuola, Jyrki; Franssila, Sami; Jokinen, Ville

doi:10.3390/mi11110979

Cited by 10 publications

(10 citation statements)

References 35 publications

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“…[ 70 ] Previous studies have employed flow of oxygen scavengers such as sodium sulfite in the channels, incorporated on‐off gas mixers onto microfluidic chips or carried out chemical or electrolytic reactions in channels to generate O 2 gradients as a means to control the O 2 concentration and generate hypoxic culture conditions coupled with microfluidics. [ 71 ] Furthermore, compartmentalized microfluidic systems involving multiple cell types allowing for cell to cell interaction, [ 72 ] along with hypoxic culture conditions and even mechanical stimulation, could enable the recapitulation of the MSC tissue niche ( Figure ).…”

Section: Combining Hypoxic Conditions With Other State Of the Art Tecmentioning

confidence: 99%

Discrepancies on the Role of Oxygen Gradient and Culture Condition on Mesenchymal Stem Cell Fate

Sámal

Rangasami

Samanta

et al. 2021

Adv Healthcare Materials

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Over the past few years, mesenchymal stem (or stromal) cells (MSCs) have garnered enormous interest due to their therapeutic value especially for their multilineage differentiation potential leading to regenerative medicine applications. MSCs undergo physiological changes upon in vitro expansion resulting in expression of different receptors, thereby inducing high variabilities in therapeutic efficacy. Therefore, understanding the biochemical cues that influence the native local signals on differentiation or proliferation of these cells is very important. There have been several reports that in vitro culture of MSCs in low oxygen gradient (or hypoxic conditions) upregulates the stemness markers and promotes cell proliferation in an undifferentiated state, as hypoxia mimics the conditions the progenitor cells experience within the tissue. However, different studies report different oxygen gradients and culture conditions causing ambiguity in their interpretation of the results. In this progress report, it is aimed to summarize recent studies in the field with specific focus on conflicting results reported during the application of hypoxic conditions for improving the proliferation or differentiation of MSCs. Further, it is tried to decipher the factors that can affect characteristics of MSC under hypoxia and suggest a few techniques that could be combined with hypoxic cell culture to better recapitulate the MSC tissue niche.

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Section: Combining Hypoxic Conditions With Other State Of the Art Tecmentioning

confidence: 99%

Discrepancies on the Role of Oxygen Gradient and Culture Condition on Mesenchymal Stem Cell Fate

Sámal

Rangasami

Samanta

et al. 2021

Adv Healthcare Materials

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show abstract

“…Another chip, described by Barmaki et al, utilizes two separate, but interdigitated microfluidic channels. The hypoxic microenvironment was created by sodium sulfite as an O 2 scavenger in one of the channels and started to increase after 100 min of pumping in the single channel [74]. Mathematical simulations contribute to support O 2 diffusion measurements rendering this kind of system very accurate [75].…”

Section: Methods Providing Physical Hypoxia Conditions: Hypoxic Chambers Tri-gas Incubator and Hypoxic Worktationmentioning

confidence: 99%

Insight into Hypoxia Stemness Control

Mattia

Mauro

Citeroni

et al. 2021

Cells

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Recently, the research on stemness and multilineage differentiation mechanisms has greatly increased its value due to the potential therapeutic impact of stem cell-based approaches. Stem cells modulate their self-renewing and differentiation capacities in response to endogenous and/or extrinsic factors that can control stem cell fate. One key factor controlling stem cell phenotype is oxygen (O2). Several pieces of evidence demonstrated that the complexity of reproducing O2 physiological tensions and gradients in culture is responsible for defective stem cell behavior in vitro and after transplantation. This evidence is still worsened by considering that stem cells are conventionally incubated under non-physiological air O2 tension (21%). Therefore, the study of mechanisms and signaling activated at lower O2 tension, such as those existing under native microenvironments (referred to as hypoxia), represent an effective strategy to define if O2 is essential in preserving naïve stemness potential as well as in modulating their differentiation. Starting from this premise, the goal of the present review is to report the status of the art about the link existing between hypoxia and stemness providing insight into the factors/molecules involved, to design targeted strategies that, recapitulating naïve O2 signals, enable towards the therapeutic use of stem cell for tissue engineering and regenerative medicine.

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“…Therefore, it is important to take this element into consideration when studying the crosstalk between cancer and immune cells and the efficacy of immunotherapy. Microfluidic chips offer this feature either through placing the chips in a hypoxia chamber or through passing sodium sulfite as the oxygen scavenger under the chips ( 97 ).…”

Section: Microfluidic Chip Technology In Head and Neck Squamous Cell ...mentioning

confidence: 99%

In vitro models as tools for screening treatment options of head and neck cancer

et al. 2022

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Various in vitro models using primary and established 2- and 3-dimensional cultures, multicellular tumor spheroids, standardized tumor slice cultures, tumor organoids, and microfluidic systems obtained from tumor lesions/biopsies of head and neck cancer (HNC) have been employed for exploring and monitoring treatment options. All of these in vitro models are to a different degree able to capture the diversity of tumors, recapitulate the disease genetically, histologically, and functionally and retain their tumorigenic potential upon xenotransplantation. The models were used for the characterization of the malignant features of the tumors and for in vitro screens of drugs approved for the treatment of HNC, including chemotherapy and radiotherapy as well as recently developed targeted therapies and immunotherapies, or for novel treatments not yet licensed for these tumor entities. The implementation of the best suitable model will enlarge our knowledge of the oncogenic properties of HNC, expand the drug repertoire and help to develop individually tailored treatment strategies resulting in the translation of these findings into the clinic. This review summarizes the different approaches using preclinical in vitro systems with their advantages and disadvantages and their implementation as preclinical platforms to predict disease course, evaluate biomarkers and test therapy efficacy.

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A Microfluidic Chip Architecture Enabling a Hypoxic Microenvironment and Nitric Oxide Delivery in Cell Culture

Cited by 10 publications

References 35 publications

Discrepancies on the Role of Oxygen Gradient and Culture Condition on Mesenchymal Stem Cell Fate

Discrepancies on the Role of Oxygen Gradient and Culture Condition on Mesenchymal Stem Cell Fate

Insight into Hypoxia Stemness Control

In vitro models as tools for screening treatment options of head and neck cancer

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