A microfluidic bubble trap and oscillator

Stucki, Janick; Guénat, O.

doi:10.1039/c5lc00592b

Cited by 32 publications

(19 citation statements)

References 12 publications

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“…This new lung-on-chip device, with a passive exchange mechanism, is simple to handle and enables long-term breathing of co-cultures at air–liquid interface, up to 22 days. Furthermore, because no external tubing or pumps are used for the perfusion, the risks of contamination, leakage, and air bubbles are nearly eliminated 46 , 49 , 50 . Additionally, the semi-open design makes it easy to create and maintain air–liquid cell culture conditions, which is usually difficult inside small microfluidic channels in combination with elastic membranes due to high surface tension forces 51 .…”

Section: Resultsmentioning

confidence: 99%

Medium throughput breathing human primary cell alveolus-on-chip model

Stucki

Hobi

Galimov

et al. 2018

Sci Rep

Self Cite

142

145

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Organs-on-chips have the potential to improve drug development efficiency and decrease the need for animal testing. For the successful integration of these devices in research and industry, they must reproduce in vivo contexts as closely as possible and be easy to use. Here, we describe a ‘breathing’ lung-on-chip array equipped with a passive medium exchange mechanism that provide an in vivo-like environment to primary human lung alveolar cells (hAEpCs) and primary lung endothelial cells. This configuration allows the preservation of the phenotype and the function of hAEpCs for several days, the conservation of the epithelial barrier functionality, while enabling simple sampling of the supernatant from the basal chamber. In addition, the chip design increases experimental throughput and enables trans-epithelial electrical resistance measurements using standard equipment. Biological validation revealed that human primary alveolar type I (ATI) and type II-like (ATII) epithelial cells could be successfully cultured on the chip over multiple days. Moreover, the effect of the physiological cyclic strain showed that the epithelial barrier permeability was significantly affected. Long-term co-culture of primary human lung epithelial and endothelial cells demonstrated the potential of the lung-on-chip array for reproducible cell culture under physiological conditions. Thus, this breathing lung-on-chip array, in combination with patients’ primary ATI, ATII, and lung endothelial cells, has the potential to become a valuable tool for lung research, drug discovery and precision medicine.

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Section: Resultsmentioning

confidence: 99%

Medium throughput breathing human primary cell alveolus-on-chip model

Stucki

Hobi

Galimov

et al. 2018

Sci Rep

Self Cite

142

145

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show abstract

“…To tackle leaks and clogs, it would be worthwhile to incorporate bubble traps, on-chip pumps, valves, and built-in sensors; these are just a few examples of improvements that could be applied to microfluidics for intestinal research. [145][146][147][148][149][150] The small size of microfluidic devices can also exacerbate difficulties in handling. For applications where neither the cells nor the reagents are especially expensive or rare, larger devices could be used.…”

Section: In Vitro Models Of the Small Intestine 321mentioning

confidence: 99%

In VitroModels of the Small Intestine: Engineering Challenges and Engineering Solutions

Hewes

Wilson

Estes

et al. 2020

Tissue Engineering Part B: Reviews

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Pathologies affecting the small intestine contribute significantly to the disease burden of both the developing and the developed world, which has motivated investigation into the disease mechanisms through in vitro models. Although existing in vitro models recapitulate selected features of the intestine, various important aspects have often been isolated or omitted due to the anatomical and physiological complexity. The small intestine's intricate microanatomy, heterogeneous cell populations, steep oxygen gradients, microbiota, and intestinal wall contractions are often not included in in vitro experimental models of the small intestine, despite their importance in both intestinal biology and pathology. Known and unknown interdependencies between various physiological aspects necessitate more complex in vitro models. Microfluidic technology has made it possible to mimic the dynamic mechanical environment, signaling gradients, and other important aspects of small intestinal biology. This review presents an overview of the complexity of small intestinal anatomy and bioengineered models that recapitulate some of these physiological aspects. Keywords: gut-on-a-chip, microfluidic models of intestine, small intestine model, tissue engineered intestine Impact Statement There is a need for improved cell culture models of the small intestine. To develop such models, it is necessary to give engineers a better understanding of intestinal biology, and conversely, to give biologists an idea of what is possible with microfluidic devices and biomaterial platforms. This work provides a detailed overview of aspects of intestinal biology that are important for tissue engineers to understand when designing models of the small intestine and provides a summary of current approaches. This review highlights both unique and common features of various strategies for modeling the intestinal cell environment, not only using microfluidic chip-based systems but also several elegant designs that use a materials-based static culture approach.

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“…The design and modeling were done by taking into consideration the surface tension and hydrodynamic forces. The study sheds light on the avenues for creating microfluidic oscillator as a consequence of the observed sinusoidal oscillations of the trapped bubble …”

Section: Superaerophilicity and Superaerophobicitymentioning

confidence: 89%

Recent Progress in Fabricating Superaerophobic and Superaerophilic Surfaces

George

Chidangil

George

2017

Adv Materials Inter

102

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In spite of large amount of research in fabricating surfaces of varying wettability by biomimicking the naturally occurring surfaces, the work on fundamentally and industrially important air bubble adhesion on solid surfaces and its applications are still in the burgeoning stage. The present progress report provides a discussion and a critical evaluation of the recent literature available on the fabrication of superaerophilic/superaerophobic surfaces via synergic modification of surface topography and surface chemistry. An abridge on the physics behind bubble wetting on a solid in a liquid medium is deciphered here, considering the interfacial surface tension balance at the three‐phase contact line, Laplace pressure, hydrostatic pressure, and surface forces, respectively. Emphasis is made on the advancement in micro/nanofabrication technologies to fabricate surfaces that break the conventional wisdom of complementary behavior of water and air bubble contact angles. The progress report presented here also shines light on the mechanism of air bubble dynamics on solid surfaces and the latest developments in achieving surfaces of varied air bubble adhesion and its applications. Finally, the prospects of the superaerophobic and superaerophilic surfaces in the near future together with the challenges faced in accomplishing them are also insinuated.

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A microfluidic bubble trap and oscillator

Cited by 32 publications

References 12 publications

Medium throughput breathing human primary cell alveolus-on-chip model

Medium throughput breathing human primary cell alveolus-on-chip model

In VitroModels of the Small Intestine: Engineering Challenges and Engineering Solutions

Recent Progress in Fabricating Superaerophobic and Superaerophilic Surfaces

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