A microelectronic conductimetric biosensor

Watson, L.D.; Maynard, Philip; Cullen, David C.; Sethi, R. S.; Brettle, J.; Lowe, Christopher R.

doi:10.1016/s0265-928x(87)80003-2

Cited by 98 publications

(27 citation statements)

References 19 publications

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“…The admittance increased with increasing KCl concentration. But the signal was dominated by the susceptance, whereas conductance changed little with increasing KCl concentration, similar to that observed by Watson et al [1]. This result can be attributed to the present IDA electrode geometry where the electrode area is relatively large compared to the small distance between the band electrode arrays.…”

Section: Resultssupporting

confidence: 88%

Microfabricated Conductometric Urea Biosensor Based on Sol-Gel Immobilized Urease

Lee

Kim

et al. 2000

Electroanalysis

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A conductometric biosensor was constructed with a sol-gel immobilized urease on a microfabricated interdigitated array (IDA) gold electrode. Tetramethoxysilane (TMOS) was used as a sol-gel precursor for the immobilization of the urease and the performance of the resulting urea biosensor was controlled through the water content in the acid-catalyzed hydrolysis of the sol-gel stock solution. The sol-gelderived urea biosensor showed relatively wide dynamic range of 0.2±50 mM in 5 mM imidazole-HC1 buffer at pH 7.5. Furthermore, the urea biosensor exhibited good sensor-to-sensor reproducibility (6.5 %) and storage stability (50 % of its original activity retained after 3 weeks) when stored in 5 mM imidazole-HC1 buffer at 4 C. The urea biosensor was successfully applied for the urea determination in urine sample by employing the differential measurement format consisting of an active IDA conductometric sensor containing sol-gel-immobilized urease and a reference IDA conductometric sensor containing bovine serum albumin layer instead of the urease.

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Section: Resultssupporting

confidence: 88%

Microfabricated Conductometric Urea Biosensor Based on Sol-Gel Immobilized Urease

Lee

Kim

et al. 2000

Electroanalysis

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“…Some enzymatic reactions may be monitored by ion conductometric or impedimetric devices [78][79][80] . In the case of conductometric biosensors the analytical information is obtained by measuring of electrolyte conductivity 2 .…”

Section: Electrochemical Biosensorsmentioning

confidence: 99%

Cholinesterase-based biosensors

Štěpánková

Vorčáková

2016

Journal of Enzyme Inhibition and Medicinal Chemistry

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Recently, cholinesterase-based biosensors are widely used for assaying anticholinergic compounds. Primarily biosensors based on enzyme inhibition are useful analytical tools for fast screening of inhibitors, such as organophosphates and carbamates. The present review is aimed at compilation of the most important facts about cholinesterase based biosensors, types of physico-chemical transduction, immobilization strategies and practical applications.

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“…Возможности экспресс-анализатора были продемонстрированы на примере реализации уреазного кондуктометрического биосенсо-ра для определения мочевины, содержание в крови которой является важным клиничес-ким фактором, характеризующим функцио-нирование почек [14]. При анализе мочевины биосенсорным методом последняя проникает в биоселективную мембрану и в результате ферментативного гидролиза расщепляется до двух ионов аммония и иона бикарбоната [15] Основой кондуктометрического способа определения мочевины являются генерируемые в результате ферментной реакции ионы, из-меняющие проводимость раствора внутри биомембраны.…”

Section: определение содержания мочевины в физиологических растворахunclassified

Express analyzer of functional materials parameters based on the impedance spectroscopy method

Кукла¹,

Мамикін²,

Maistrenko³

et al. 2012

SEMST

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A microelectronic conductimetric biosensor

Cited by 98 publications

References 19 publications

Microfabricated Conductometric Urea Biosensor Based on Sol-Gel Immobilized Urease

Microfabricated Conductometric Urea Biosensor Based on Sol-Gel Immobilized Urease

Cholinesterase-based biosensors

Express analyzer of functional materials parameters based on the impedance spectroscopy method

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