A Microchip For Studying the Effects of Dopamine and its Precursor On Neurospheroids

Mao, Subin; Fonder, Catherine; Rubby, Fazlay; Yang, Renyuan; Phillips, Gregory; Sakaguchi, Donald S.; Que, Long

doi:10.1109/mems51670.2022.9699585

Cited by 1 publication

(1 citation statement)

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“…The adjacent misaligned rows of the micropillars reduce the effective gaps among them. As a result, the fluidic resistance increases, and the passage of AHPC-NSs through the diffusion barrier to the sensor chambers can be significantly minimized; thus the biofouling issues associated with the cells and cellular debris of the sensors can be tremendously mitigated. The gaps among micropillars in the same row or between each row are designed to be 20 μm.…”

Section: Methodsmentioning

confidence: 99%

Assessment of the Behaviors of an In Vitro Brain Model On-Chip under Shockwave Impacts

Rubby,

Fonder,

Uchayash

et al. 2024

ACS Appl. Mater. Interfaces

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Herein we report the assessment of the effects of shockwave (SW) impacts on adult rat hippocampal progenitor cell (AHPC) neurospheres (NSs), which are used as in vitro brain models, for enhancing our understanding of the mechanisms of traumatic brain injury (TBI). The assessment has been achieved by using culture dishes and a new microchip. The microchip allows the chemicals released from the brain models cultured inside the cell culture chamber under SW impacts to diffuse to the nanosensors in adjacent sensor chambers through built-in diffusion barriers, which are used to prevent the cells from entering the sensor chambers, thereby mitigating the biofouling issues of the sensor surface. Experiments showed the negative impact of the SW on the viability, proliferation, and differentiation of the cells within the NSs. A qPCR gene expression analysis was performed and appeared to confirm some of the immunocytochemistry (ICC) results. Finally, we demonstrated that the microchip can be used to monitor lactate dehydrogenase (LDH) released from the AHPC-NSs subjected to SW impacts. As expected, LDH levels changed when AHPC-NSs were injured by SW impacts, verifying this chip can be used for assessing the degrees of injuries to AHPC-NSs by monitoring LDH levels. Taken together, these results suggest the feasibility of using the chip to better understand the interactions between SW impacts and in vitro brain models, paving the way for potentially establishing in vitro TBI models on a chip.

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Section: Methodsmentioning

confidence: 99%