A microchip for electroporation of primary endothelial cells

Lin, Yu-Cheng; Li, Min; Fan, Chun Sheng; Wu, Li-Wha

doi:10.1016/j.sna.2003.05.002

Cited by 56 publications

(37 citation statements)

References 18 publications

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“…Besides this advantage, only small amounts of transfection material are needed, and it is possible to make structures where the transfection of more cells in a parallel fashion is possible. [64,68,69] Several microfluidic designs have been published, some aiming at single-cell transfection, others at the transfection of larger amounts of cells.…”

Section: Transfection Designsmentioning

confidence: 99%

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Electroporation of cells in microfluidic devices: a review

et al. 2006

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In recent years, several publications on microfluidic devices have focused on the process of electroporation, which results in the poration of the biological cell membrane. The devices involved are designed for cell analysis, transfection or pasteurization. The high electric field strengths needed are induced by placing the electrodes in close proximity or by creating a constriction between the electrodes, which focuses the electric field. Detection is usually achieved through fluorescent labeling or by measuring impedance. So far, most of these devices have only concerned themselves solely with the electroporation process, but integration with separation and detection processes is expected in the near future. In particular, single-cell content analysis is expected to add further value to the concept of the microfluidic chip. Furthermore, if advanced pulse schemes are employed, such microdevices can also enhance research into intracellular electroporation.

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Section: Transfection Designsmentioning

confidence: 99%

“…While all the transfection-oriented designs above were aimed at cells in solution, Lin et al [64,68,69] created a microfluidic design aimed at the transfection of animal cells growing on a solid surface (Fig. 3d).…”

Section: Transfection Designsmentioning

confidence: 99%

Electroporation of cells in microfluidic devices: a review

et al. 2006

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“…Recently we made a breakthrough in the design and fabrication of the in vitro EP microchips (Huang et al 2007;Jen et al 2004;Lin et al , 2003Li et al 2006). In those studies, we focus on the development of microdevices for gene delivery to cells.…”

Section: Introductionmentioning

confidence: 99%

An electroporation microchip system for the transfection of zebrafish embryos using quantum dots and GFP genes for evaluation

Huang¹,

Lin²,

Su³

et al. 2007

Biomed Microdevices

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This study focuses on the design and experimental verification of an electroporation (EP) microchip system for the transfection of zebrafish (Danio rerio). For generating suitable pulses, a circuit is used to provide voltages between 0 and 700 V, with nearly 0-3,500 V/cm electric field. In addition, a proposed EP microchip, designed in a modular fashion, is fabricated using micro electromechanical system (MEMS) technology to allow for rapid and convenient replacement of each component. A numerical simulation is carried out to analyze the uniformity and strength of the EP electric fields generated in the microchip. Trypan blue dye, water-soluble quantum dots (MUA-QDs) and genes coding for green fluorescence protein (pEGFP-N1 plasmids) were employed to verify the successful delivery and transfection of zebrafish embryos. The experimental results show that the optimum delivery rate of trypan blue dyes and MUA-QDs were respectively up to 62 and 36% by using the proposed EP system. The successfully transfected embryos with the pEGFP-N1 plasmid used exhibit green fluorescence in the zebrafish embryos. The approach in the transfection of zebrafish embryos will provide many potential usages for cellular imaging areas, gene therapy research and medical applications.

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“…The permeabilization area can be controlled with the pulse amplitude and the degree of permeabilization can be controlled with the duration of pulses, numbers of pulses, where longer pulses provide a larger perturbation area in the cell membrane [34,35]. In earlier studies of micro/nanofluidic based single cell electroporation, authors analyze cellular content and cellular properties [36][37][38][39], transfection of cells [17,[40][41][42] and inactivating cells [43][44][45] with the use of micro-channel based electroporation [46][47][48][49], micro-capillary based electroporation [50][51][52], electroporation with solid microelectrode [36,[53][54][55], membrane sandwich based microfluidic electroporation [56,57], microarray single cell electroporation [58], optofluidic based microfluidic devices [59][60][61][62][63][64][65], etc. Table 1 describes in detail micro/nanofluidic based single cell transfection, cell lysis, cell type with species, potential difference, pulse duration, etc.…”

Section: Micro/nanofluidic Devices For Single Cell Electroporationmentioning

confidence: 99%

Recent Trends on Micro/Nanofluidic Single Cell Electroporation

Santra

Tseng

2013

Micromachines

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Abstract:The behaviors of cell to cell or cell to environment with their organelles and their intracellular physical or biochemical effects are still not fully understood. Analyzing millions of cells together cannot provide detailed information, such as cell proliferation, differentiation or different responses to external stimuli and intracellular reaction. Thus, single cell level research is becoming a pioneering research area that unveils the interaction details in high temporal and spatial resolution among cells. To analyze the cellular function, single cell electroporation can be conducted by employing a miniaturized device, whose dimension should be similar to that of a single cell. Micro/nanofluidic devices can fulfill this requirement for single cell electroporation. This device is not only useful for cell lysis, cell to cell fusion or separation, insertion of drug, DNA and antibodies inside single cell, but also it can control biochemical, electrical and mechanical parameters using electroporation technique. This device provides better performance such as high transfection efficiency, high cell viability, lower Joule heating effect, less sample contamination, lower toxicity during electroporation experiment when compared to bulk electroporation process. In addition, single organelles within a cell can be analyzed selectively by reducing the electrode size and gap at nanoscale level. This advanced technique can deliver (in/out) biomolecules precisely through a small membrane area (micro to nanoscale area) of the single cell, known as localized single cell membrane electroporation (LSCMEP). These articles emphasize the recent progress in micro/nanofluidic single cell electroporation, which is potentially beneficial for OPEN ACCESSMicromachines 2013, 4 334 high-efficient therapeutic and delivery applications or understanding cell to cell interaction.

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A microchip for electroporation of primary endothelial cells

Cited by 56 publications

References 18 publications

Electroporation of cells in microfluidic devices: a review

Electroporation of cells in microfluidic devices: a review

An electroporation microchip system for the transfection of zebrafish embryos using quantum dots and GFP genes for evaluation

Recent Trends on Micro/Nanofluidic Single Cell Electroporation

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