A microbiological six-plate method for the identification of certain antibiotic groups in incurred kidney and muscle samples

Myllyniemi, Anna-Liisa; Nuotio, L.; Lindfors, E.; Rannikko, Riitta; Niemi, Anneli; Bäckman, Christina

doi:10.1039/b100135n

Cited by 63 publications

(51 citation statements)

References 11 publications

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“…These methods use various strains of E. coli and substrates of various pH, e.g. E. coli Bayer 14 at pH 6 (Okerman et al 2001), E. coli RIK 144 at pH 6 (Nouws et al 1999b), E. coli ATCC 11303 at pH 7.2 (Myllyniemi et al 2001) and/or at pH 8 (Gaudin et al 2004). According to Choi et al (1999), the E. coli strain ATCC 128 was highly suitable as an indicator of microorganisms in a microbial inhibition test for selective detection of fluoroquinolone residues in animal tissues.…”

Section: Resultsmentioning

confidence: 99%

A plate diffusion method for detecting fluoroquinolone residues in raw cow's milk

Navrátilová¹,

Vyhnálková²,

Vorlová³

et al. 2014

Czech J. Food Sci.

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Navrátilová P., Vyhnálková J., Vorlová L., Jeřábková J. (2014): A plate diffusion method for detecting fluoroquinolone residues in raw cow's milk. Czech J. Food Sci., 32: 260-264.The plate diffusion method is a reference method in the Czech Republic for determination of residues of antimicrobial agents in raw materials and foodstuffs of animal origin. A new method using the E. coli strain ATCC 11303 for the detection of fluoroquinolones was introduced in 2008. The aim of this study was to determine the detection capability (CCβ) of this modified method using this E. coli strain for selected fluoroquinolones registered in the Czech Republic for treating diseases in cattle -danofloxacin, marbofloxacin, ciprofloxacin, enrofloxacin, and flumequine. When comparing the maximum residue limits for individual fluoroquinolones and the CCβ values determined, we can state that the method displays very good sensitivity to ciprofloxacin and enrofloxacin (20 and 40 µg/l), marbofloxacin (70 µg/l), and danofloxacin (30 µg/l). The CCβ of the method for flumequine was not found in concentrations ≤ MRL. The method did not display sensitivity to flumequine even in a concentration equal to twelve times the MRL.

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Section: Resultsmentioning

confidence: 99%

A plate diffusion method for detecting fluoroquinolone residues in raw cow's milk

Navrátilová¹,

Vyhnálková²,

Vorlová³

et al. 2014

Czech J. Food Sci.

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“…Analytical methods described in the literature for the analysis of quinolones in foods include instrumental techniques (Hernández-Arteseros et al 1998;Rose et al 1998;Roybal et al 2002), rapid immunological techniques (Tittlemier et al 2008;Scortichini et al 2009) and microbial methods (Currie et al 1998;Pikkemaat et al 2007;Fuselier et al 2000;Calderón et al 1996;Myllyniemi et al 2001;Cantwell and O'keeffe 2006;Gaudin et al 2009). …”

Section: Introductionmentioning

confidence: 98%

Performance of a New Microbial Test for Quinolone Residues in Muscle

Sanz¹,

Mata²,

Condón

et al. 2010

Food Anal. Methods

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Concerns regarding the presence of drug residues in foods include allergic reactions, toxicity, technological problems in fermented products and the development of antibiotic resistance in human pathogens. The analysis of antimicrobial residues in foods is generally carried out, in a first step, through microbiological screening tests. These tests commonly use Geobacillus stearothermophilus as target specie but show a low ability to detect quinolones. The goal of our study was to evaluate the performance of a new microbiological test (Equinox) for detection of quinolone residues in muscle. The kit contains an ampoule with a standardized number of freeze-dried Escherichia coli and must be diluted with a specific detection medium containing a redox indicator. Microbial growth will modify the redox potential of the medium being observed through a colour change (from blue to brown/orange). Equinox limits of detection for most of tested quinolones (enrofloxacin, norfloxacin, sarafloxacin, marbofloxacin, ciprofloxacin, danofloxacin and difloxacin) were below or around maximum residue limit (MRL) UE and CCβ values obtained corresponded with the determined sensitivities. In contrast, flumequine could not be detected at MRL UE levels. Moreover, Equinox displayed a low sensitivity to other antimicrobials. Sensitivity data obtained in vitro were consistent when testing incurred muscle samples. Matrix constituents, test batch and animal species did not affect the performance of the test. Equinox could be easily automated enabling a large numbers of simultaneous analysis, and a photometric reading can be applied for a precise interpretation. The results obtained in this study prove that Equinox is a useful tool when screening for quinolone residues or can be combined with other methods for screening of unknown samples.

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“…Thus, it should be noted that the TMP levels used for the bioassay preparation were higher than 200 and 60 µg/l used by Koenen-Dierick and De Beer (1998) and Myllyniemi et al (2001), respectively, for the diffusion tests in Petri dishes with B. subtilis BGA.…”

Section: Effect Of Trimethoprim Concentration On the Specificity And mentioning

confidence: 99%

Use of chemometric techniques to design a microbiological method for sulfonamide detection in milk

Nagel¹,

Molina²,

Althaus³

2013

Czech J. Food Sci.

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Nagel O.G., Molina M.P., Althaus R.L. (2013): Use of chemometric techniques to design a microbiological method for sulfonamide detection in milk. Czech J. Food Sci., 31: 627-632.We proposed an experimental design of a microbial bioassay of dichotomous response (positive or negative) using Bacillus subtilis BGA for the detection of sulfonamide residues. In the first stage, the bioassay response time was reduced to 6 h by increasing the spore concentration of B. subtilis. Then, the effects of spore, indicator, trimethoprim (TMP) concentration, and volume of the culture medium were examined with a Plackett Burman design (2 4-1 ). Finally, the effect of TMP concentration on the method detection capabilities and specificity was analysed using a logistic model with interaction. The detection capabilities of sulfonamides in milk are close to the MRLs when using 500 mg/l of TMP in the culture medium of the bioassay. It is concluded that the experimental design techniques and a logistic regression model can be used to design successfully a dichotomous response bioassay.

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A microbiological six-plate method for the identification of certain antibiotic groups in incurred kidney and muscle samples

Cited by 63 publications

References 11 publications

A plate diffusion method for detecting fluoroquinolone residues in raw cow's milk

A plate diffusion method for detecting fluoroquinolone residues in raw cow's milk

Performance of a New Microbial Test for Quinolone Residues in Muscle

Use of chemometric techniques to design a microbiological method for sulfonamide detection in milk

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